Names & Taxonomy
- Uniprot ID:
- Q13564
- Entry Name:
- ULA1_HUMAN
- Status:
- reviewed
- Protein Names:
- NEDD8-activating enzyme E1 regulatory subunit (Amyloid beta precursor protein-binding protein 1, 59 kDa) (APP-BP1) (Amyloid protein-binding protein 1) (Proto-oncogene protein 1)
- Gene Names:
- NAE1 APPBP1 HPP1
- Gene Names Orf:
- HPP1
- Gene Names Primary:
- NAE1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 534
- Sequence:
- MAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cell membrane
Function
- Function:
- Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
- Pathway:
- Protein modification; protein neddylation.
- Enzyme Regulation:
- ENZYME REGULATION: Binding of TP53BP2 to the regulatory subunit NAE1 decreases neddylation activity.
- Cross Reference Drug Bank:
- DB00171
- Gene Ontology Go:
- cytoplasm
cytosol
nucleus
plasma membrane
NEDD8 activating enzyme activity
protein heterodimerization activity
ubiquitin protein ligase binding
mitotic DNA replication checkpoint
neuron apoptotic process
protein neddylation
regulation of apoptotic process
regulation of neuron apoptotic process
signal transduction - Gene Ontology Biological Process:
- mitotic DNA replication checkpoint
neuron apoptotic process
protein neddylation
regulation of apoptotic process
regulation of neuron apoptotic process
signal transduction - Gene Ontology Molecular Function:
- NEDD8 activating enzyme activity
protein heterodimerization activity
ubiquitin protein ligase binding - Gene Ontology Cellular Component:
- cytoplasm
cytosol
nucleus
plasma membrane - Keywords:
- 3D-structure
Acetylation
Alternative splicing
Apoptosis
Cell cycle
Cell membrane
Complete proteome
Membrane
Polymorphism
Reference proteome
Ubl conjugation
Ubl conjugation pathway