Names & Taxonomy
- Uniprot ID:
- Q9NR71
- Entry Name:
- ASAH2_HUMAN
- Status:
- reviewed
- Protein Names:
- Neutral ceramidase (N-CDase) (NCDase) (EC 3.5.1.23) (Acylsphingosine deacylase 2) (BCDase) (LCDase) (hCD) (N-acylsphingosine amidohydrolase 2) (Non-lysosomal ceramidase) [Cleaved into: Neutral ceramidase soluble form]
- Gene Names:
- ASAH2 HNAC1
- Gene Names Primary:
- ASAH2
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 780
- Sequence:
- MAKRTFSNLETFLIFLLVMMSAITVALLSLLFITSGTIENHKDLGGHFFSTTQSPPATQGSTAAQRSTATQHSTATQSSTATQTSPVPLTPESPLFQNFSGYHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQDMFDSTQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDVTVWLNSTHASKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGMQNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIATDTVANLSRGPEPPFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKGLLGLSNATVEWHIPDTAQPGIYRIRYFGHNRKQDILKPAVILSFEGTSPAFEVVTI
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cell membrane
Function
- Function:
- Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.
- Catalytic Activity:
- N-acylsphingosine + H(2)O = a carboxylate + sphingosine.
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=71.4 uM for octanoyl-sphingosine
- Enzyme Regulation:
- ENZYME REGULATION: Inhibited by dithiothreitol (DTT), 2-mercaptoethanol, Zn(2+), Cu(2+) and Fe(2+). Enhanced by Na(+) and Ca(2+), and at lower level Mg(2+) and Mn(2+).
- Active Site:
- ACT_SITE 354 354 Nucleophile.
- Gene Ontology Go:
- integral component of membrane
mitochondrion
plasma membrane
ceramidase activity
apoptotic process
ceramide metabolic process
signal transduction - Gene Ontology Biological Process:
- apoptotic process
ceramide metabolic process
signal transduction - Gene Ontology Molecular Function:
- ceramidase activity
- Gene Ontology Cellular Component:
- integral component of membrane
mitochondrion
plasma membrane - Keywords:
- 3D-structure
Alternative splicing
Apoptosis
Cell membrane
Complete proteome
Glycoprotein
Hydrolase
Lipid metabolism
Membrane
Phosphoprotein
Polymorphism
Reference proteome
Signal-anchor
Sphingolipid metabolism
Transmembrane
Transmembrane helix
Ubl conjugation