Names & Taxonomy

Uniprot ID:
Q16647
Entry Name:
PTGIS_HUMAN
Status:
reviewed
Protein Names:
Prostacyclin synthase (EC 5.3.99.4) (Prostaglandin I2 synthase)
Gene Names:
PTGIS CYP8 CYP8A1
Gene Names Primary:
PTGIS
Organism:
Homo sapiens (Human)

Structure

Length:
500
Sequence:
MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMKEKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLRLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVEIPEFDLSRYGFGLMQPEHDVPVRYRIRP
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Endoplasmic reticulum membrane; Single-pass membrane protein.

Function

Function:
Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).
Catalytic Activity:
(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-6,9-alpha-epoxy-11-alpha,15-dihydroxyprosta-5,13-dienoate.
Cofactor:
COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413;
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=9.55 uM for 6-keto-PGF1alpha
Cross Reference Drug Bank:
DB01240 DB00812
Gene Ontology Go:
caveola
endoplasmic reticulum
endoplasmic reticulum membrane
extracellular space
integral component of membrane
nucleus
heme binding
iron ion binding
monooxygenase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
prostaglandin-I synthase activity
apoptotic signaling pathway
arachidonic acid metabolic process
cellular response to hypoxia
cellular response to interleukin-1
cellular response to interleukin-6
cyclooxygenase pathway
decidualization
embryo implantation
icosanoid metabolic process
NAD metabolic process
negative regulation of inflammatory response
negative regulation of NF-kappaB transcription factor activity
negative regulation of nitric oxide biosynthetic process
nicotinamide metabolic process
positive regulation of angiogenesis
positive regulation of execution phase of apoptosis
positive regulation of peroxisome proliferator activated receptor signaling pathway
prostaglandin biosynthetic process
small molecule metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process
xenobiotic metabolic process
Gene Ontology Biological Process:
apoptotic signaling pathway
arachidonic acid metabolic process
cellular response to hypoxia
cellular response to interleukin-1
cellular response to interleukin-6
cyclooxygenase pathway
decidualization
embryo implantation
icosanoid metabolic process
NAD metabolic process
negative regulation of inflammatory response
negative regulation of NF-kappaB transcription factor activity
negative regulation of nitric oxide biosynthetic process
nicotinamide metabolic process
positive regulation of angiogenesis
positive regulation of execution phase of apoptosis
positive regulation of peroxisome proliferator activated receptor signaling pathway
prostaglandin biosynthetic process
small molecule metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process
xenobiotic metabolic process
Gene Ontology Molecular Function:
heme binding
iron ion binding
monooxygenase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
prostaglandin-I synthase activity
Gene Ontology Cellular Component:
caveola
endoplasmic reticulum
endoplasmic reticulum membrane
extracellular space
integral component of membrane
nucleus
Keywords:
3D-structure
Complete proteome
Endoplasmic reticulum
Fatty acid biosynthesis
Fatty acid metabolism
Heme
Iron
Isomerase
Lipid biosynthesis
Lipid metabolism
Membrane
Metal-binding
Polymorphism
Prostaglandin biosynthesis
Prostaglandin metabolism
Reference proteome
Transmembrane
Transmembrane helix

Publication

PubMed ID:
8185632 11281454 11780052 15489334 12372404 25623425 17020766