Names & Taxonomy

Uniprot ID:
Q15067
Entry Name:
ACOX1_HUMAN
Status:
reviewed
Protein Names:
Peroxisomal acyl-coenzyme A oxidase 1 (AOX) (EC 1.3.3.6) (Palmitoyl-CoA oxidase) (Straight-chain acyl-CoA oxidase) (SCOX)
Gene Names:
ACOX1 ACOX
Gene Names Primary:
ACOX1
Organism:
Homo sapiens (Human)

Structure

Length:
660
Sequence:
MNPDLRRERDSASFNPELLTHILDGSPEKTRRRREIENMILNDPDFQHEDLNFLTRSQRYEVAVRKSAIMVKKMREFGIADPDEIMWFKKLHLVNFVEPVGLNYSMFIPTLLNQGTTAQKEKWLLSSKGLQIIGTYAQTEMGHGTHLRGLETTATYDPETQEFILNSPTVTSIKWWPGGLGKTSNHAIVLAQLITKGKCYGLHAFIVPIREIGTHKPLPGITVGDIGPKFGYDEIDNGYLKMDNHRIPRENMLMKYAQVKPDGTYVKPLSNKLTYGTMVFVRSFLVGEAARALSKACTIAIRYSAVRHQSEIKPGEPEPQILDFQTQQYKLFPLLATAYAFQFVGAYMKETYHRINEGIGQGDLSELPELHALTAGLKAFTSWTANTGIEACRMACGGHGYSHCSGLPNIYVNFTPSCTFEGENTVMMLQTARFLMKSYDQVHSGKLVCGMVSYLNDLPSQRIQPQQVAVWPTMVDINSPESLTEAYKLRAARLVEIAAKNLQKEVIHRKSKEVAWNLTSVDLVRASEAHCHYVVVKLFSEKLLKIQDKAIQAVLRSLCLLYSLYGISQNAGDFLQGSIMTEPQITQVNQRVKELLTLIRSDAVALVDAFDFQDVTLGSVLGRYDGNVYENLFEWAKNSPLNKAEVHESYKHLKSLQSKL
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Peroxisome.

Function

Function:
Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.
Pathway:
Lipid metabolism; peroxisomal fatty acid beta-oxidation.
Catalytic Activity:
Acyl-CoA + O(2) = trans-2,3-dehydroacyl-CoA + H(2)O(2).
Cofactor:
COFACTOR: Name=FAD; Xref=ChEBI:CHEBI:57692;
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=73 uM for palmitoyl-CoA (isoform 1)
Active Site:
ACT_SITE 421 421 Proton acceptor.
Cross Reference Drug Bank:
DB03147
Gene Ontology Go:
intracellular membrane-bounded organelle
membrane
mitochondrion
nucleolus
nucleoplasm
nucleus
peroxisomal matrix
peroxisomal membrane
peroxisome
plasma membrane
acyl-CoA dehydrogenase activity
acyl-CoA oxidase activity
electron carrier activity
FAD binding
fatty-acyl-CoA binding
flavin adenine dinucleotide binding
oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor
palmitoyl-CoA oxidase activity
PDZ domain binding
protein N-terminus binding
receptor binding
alpha-linolenic acid metabolic process
cellular lipid metabolic process
fatty acid beta-oxidation using acyl-CoA dehydrogenase
fatty acid beta-oxidation using acyl-CoA oxidase
fatty acid oxidation
generation of precursor metabolites and energy
lipid homeostasis
lipid metabolic process
peroxisome fission
positive regulation of cholesterol homeostasis
prostaglandin metabolic process
small molecule metabolic process
spermatogenesis
unsaturated fatty acid metabolic process
very long-chain fatty acid metabolic process
Gene Ontology Biological Process:
alpha-linolenic acid metabolic process
cellular lipid metabolic process
fatty acid beta-oxidation using acyl-CoA dehydrogenase
fatty acid beta-oxidation using acyl-CoA oxidase
fatty acid oxidation
generation of precursor metabolites and energy
lipid homeostasis
lipid metabolic process
peroxisome fission
positive regulation of cholesterol homeostasis
prostaglandin metabolic process
small molecule metabolic process
spermatogenesis
unsaturated fatty acid metabolic process
very long-chain fatty acid metabolic process
Gene Ontology Molecular Function:
acyl-CoA dehydrogenase activity
acyl-CoA oxidase activity
electron carrier activity
FAD binding
fatty-acyl-CoA binding
flavin adenine dinucleotide binding
oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor
palmitoyl-CoA oxidase activity
PDZ domain binding
protein N-terminus binding
receptor binding
Gene Ontology Cellular Component:
intracellular membrane-bounded organelle
membrane
mitochondrion
nucleolus
nucleoplasm
nucleus
peroxisomal matrix
peroxisomal membrane
peroxisome
plasma membrane
Keywords:
Acetylation
Alternative splicing
Complete proteome
Disease mutation
FAD
Fatty acid metabolism
Flavoprotein
Lipid metabolism
Oxidoreductase
Peroxisome
Phosphoprotein
Polymorphism
Reference proteome

Publication

PubMed ID:
8159712 7876265 8040306 8117268 14702039 17974005 16625196 15489334 17081983 17603022 18669648 19608861 20195242 21269460 24275569 25944712 11815777 17458872