Names & Taxonomy
- Uniprot ID:
- Q15067
- Entry Name:
- ACOX1_HUMAN
- Status:
- reviewed
- Protein Names:
- Peroxisomal acyl-coenzyme A oxidase 1 (AOX) (EC 1.3.3.6) (Palmitoyl-CoA oxidase) (Straight-chain acyl-CoA oxidase) (SCOX)
- Gene Names:
- ACOX1 ACOX
- Gene Names Primary:
- ACOX1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 660
- Sequence:
- MNPDLRRERDSASFNPELLTHILDGSPEKTRRRREIENMILNDPDFQHEDLNFLTRSQRYEVAVRKSAIMVKKMREFGIADPDEIMWFKKLHLVNFVEPVGLNYSMFIPTLLNQGTTAQKEKWLLSSKGLQIIGTYAQTEMGHGTHLRGLETTATYDPETQEFILNSPTVTSIKWWPGGLGKTSNHAIVLAQLITKGKCYGLHAFIVPIREIGTHKPLPGITVGDIGPKFGYDEIDNGYLKMDNHRIPRENMLMKYAQVKPDGTYVKPLSNKLTYGTMVFVRSFLVGEAARALSKACTIAIRYSAVRHQSEIKPGEPEPQILDFQTQQYKLFPLLATAYAFQFVGAYMKETYHRINEGIGQGDLSELPELHALTAGLKAFTSWTANTGIEACRMACGGHGYSHCSGLPNIYVNFTPSCTFEGENTVMMLQTARFLMKSYDQVHSGKLVCGMVSYLNDLPSQRIQPQQVAVWPTMVDINSPESLTEAYKLRAARLVEIAAKNLQKEVIHRKSKEVAWNLTSVDLVRASEAHCHYVVVKLFSEKLLKIQDKAIQAVLRSLCLLYSLYGISQNAGDFLQGSIMTEPQITQVNQRVKELLTLIRSDAVALVDAFDFQDVTLGSVLGRYDGNVYENLFEWAKNSPLNKAEVHESYKHLKSLQSKL
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Peroxisome.
Function
- Function:
- Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.
- Pathway:
- Lipid metabolism; peroxisomal fatty acid beta-oxidation.
- Catalytic Activity:
- Acyl-CoA + O(2) = trans-2,3-dehydroacyl-CoA + H(2)O(2).
- Cofactor:
- COFACTOR: Name=FAD; Xref=ChEBI:CHEBI:57692;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=73 uM for palmitoyl-CoA (isoform 1)
- Active Site:
- ACT_SITE 421 421 Proton acceptor.
- Cross Reference Drug Bank:
- DB03147
- Gene Ontology Go:
- intracellular membrane-bounded organelle
membrane
mitochondrion
nucleolus
nucleoplasm
nucleus
peroxisomal matrix
peroxisomal membrane
peroxisome
plasma membrane
acyl-CoA dehydrogenase activity
acyl-CoA oxidase activity
electron carrier activity
FAD binding
fatty-acyl-CoA binding
flavin adenine dinucleotide binding
oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor
palmitoyl-CoA oxidase activity
PDZ domain binding
protein N-terminus binding
receptor binding
alpha-linolenic acid metabolic process
cellular lipid metabolic process
fatty acid beta-oxidation using acyl-CoA dehydrogenase
fatty acid beta-oxidation using acyl-CoA oxidase
fatty acid oxidation
generation of precursor metabolites and energy
lipid homeostasis
lipid metabolic process
peroxisome fission
positive regulation of cholesterol homeostasis
prostaglandin metabolic process
small molecule metabolic process
spermatogenesis
unsaturated fatty acid metabolic process
very long-chain fatty acid metabolic process - Gene Ontology Biological Process:
- alpha-linolenic acid metabolic process
cellular lipid metabolic process
fatty acid beta-oxidation using acyl-CoA dehydrogenase
fatty acid beta-oxidation using acyl-CoA oxidase
fatty acid oxidation
generation of precursor metabolites and energy
lipid homeostasis
lipid metabolic process
peroxisome fission
positive regulation of cholesterol homeostasis
prostaglandin metabolic process
small molecule metabolic process
spermatogenesis
unsaturated fatty acid metabolic process
very long-chain fatty acid metabolic process - Gene Ontology Molecular Function:
- acyl-CoA dehydrogenase activity
acyl-CoA oxidase activity
electron carrier activity
FAD binding
fatty-acyl-CoA binding
flavin adenine dinucleotide binding
oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor
palmitoyl-CoA oxidase activity
PDZ domain binding
protein N-terminus binding
receptor binding - Gene Ontology Cellular Component:
- intracellular membrane-bounded organelle
membrane
mitochondrion
nucleolus
nucleoplasm
nucleus
peroxisomal matrix
peroxisomal membrane
peroxisome
plasma membrane - Keywords:
- Acetylation
Alternative splicing
Complete proteome
Disease mutation
FAD
Fatty acid metabolism
Flavoprotein
Lipid metabolism
Oxidoreductase
Peroxisome
Phosphoprotein
Polymorphism
Reference proteome