Names & Taxonomy
- Uniprot ID:
- Q00653
- Entry Name:
- NFKB2_HUMAN
- Status:
- reviewed
- Protein Names:
- Nuclear factor NF-kappa-B p100 subunit (DNA-binding factor KBF2) (H2TF1) (Lymphocyte translocation chromosome 10 protein) (Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2) (Oncogene Lyt-10) (Lyt10) [Cleaved into: Nuclear factor NF-kappa-B p52 subunit]
- Gene Names:
- NFKB2 LYT10
- Gene Names Primary:
- NFKB2
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 900
- Sequence:
- MESCYNPGLDGIIEYDDFKLNSSIVEPKEPAPETADGPYLVIVEQPKQRGFRFRYGCEGPSHGGLPGASSEKGRKTYPTVKICNYEGPAKIEVDLVTHSDPPRAHAHSLVGKQCSELGICAVSVGPKDMTAQFNNLGVLHVTKKNMMGTMIQKLQRQRLRSRPQGLTEAEQRELEQEAKELKKVMDLSIVRLRFSAFLRASDGSFSLPLKPVISQPIHDSKSPGASNLKISRMDKTAGSVRGGDEVYLLCDKVQKDDIEVRFYEDDENGWQAFGDFSPTDVHKQYAIVFRTPPYHKMKIERPVTVFLQLKRKRGGDVSDSKQFTYYPLVEDKEEVQRKRRKALPTFSQPFGGGSHMGGGSGGAAGGYGGAGGGGSLGFFPSSLAYSPYQSGAGPMGCYPGGGGGAQMAATVPSRDSGEEAAEPSAPSRTPQCEPQAPEMLQRAREYNARLFGLAQRSARALLDYGVTADARALLAGQRHLLTAQDENGDTPLHLAIIHGQTSVIEQIVYVIHHAQDLGVVNLTNHLHQTPLHLAVITGQTSVVSFLLRVGADPALLDRHGDSAMHLALRAGAGAPELLRALLQSGAPAVPQLLHMPDFEGLYPVHLAVRARSPECLDLLVDSGAEVEATERQGGRTALHLATEMEELGLVTHLVTKLRANVNARTFAGNTPLHLAAGLGYPTLTRLLLKAGADIHAENEEPLCPLPSPPTSDSDSDSEGPEKDTRSSFRGHTPLDLTCSTKVKTLLLNAAQNTMEPPLTPPSPAGPGLSLGDTALQNLEQLLDGPEAQGSWAELAERLGLRSLVDTYRQTTSPSGSLLRSYELAGGDLAGLLEALSDMGLEEGVRLLRGPETRDKLPSTAEVKEDSAYGSQSVEQEAEKLGPPPEPPGGLCHGHPQPQVH
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
Function
- Function:
- NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
- Cross Reference Drug Bank:
- DB00945 DB01296
- Gene Ontology Go:
- Bcl3/NF-kappaB2 complex
cytoplasm
cytosol
nucleoplasm
nucleus
chromatin binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription coactivator activity
transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
aging
extracellular matrix organization
follicular dendritic cell differentiation
germinal center formation
I-kappaB kinase/NF-kappaB signaling
inflammatory response
innate immune response
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
negative regulation of transcription from RNA polymerase II promoter
NIK/NF-kappaB signaling
positive regulation of NF-kappaB transcription factor activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of type I interferon production
regulation of transcription, DNA-templated
response to cytokine
response to lipopolysaccharide
rhythmic process
spleen development
stimulatory C-type lectin receptor signaling pathway
toll-like receptor 10 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
toll-like receptor 5 signaling pathway
toll-like receptor 9 signaling pathway
toll-like receptor signaling pathway
toll-like receptor TLR1:TLR2 signaling pathway
toll-like receptor TLR6:TLR2 signaling pathway
transcription from RNA polymerase II promoter
TRIF-dependent toll-like receptor signaling pathway
tumor necrosis factor-mediated signaling pathway - Gene Ontology Biological Process:
- aging
extracellular matrix organization
follicular dendritic cell differentiation
germinal center formation
I-kappaB kinase/NF-kappaB signaling
inflammatory response
innate immune response
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
negative regulation of transcription from RNA polymerase II promoter
NIK/NF-kappaB signaling
positive regulation of NF-kappaB transcription factor activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of type I interferon production
regulation of transcription, DNA-templated
response to cytokine
response to lipopolysaccharide
rhythmic process
spleen development
stimulatory C-type lectin receptor signaling pathway
toll-like receptor 10 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
toll-like receptor 5 signaling pathway
toll-like receptor 9 signaling pathway
toll-like receptor signaling pathway
toll-like receptor TLR1:TLR2 signaling pathway
toll-like receptor TLR6:TLR2 signaling pathway
transcription from RNA polymerase II promoter
TRIF-dependent toll-like receptor signaling pathway
tumor necrosis factor-mediated signaling pathway - Gene Ontology Molecular Function:
- chromatin binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription coactivator activity
transcription factor activity, sequence-specific DNA binding - Gene Ontology Cellular Component:
- Bcl3/NF-kappaB2 complex
cytoplasm
cytosol
nucleoplasm
nucleus - Keywords:
- 3D-structure
ANK repeat
Activator
Alternative splicing
Biological rhythms
Chromosomal rearrangement
Complete proteome
Cytoplasm
DNA-binding
Direct protein sequencing
Disease mutation
Isopeptide bond
Nucleus
Phosphoprotein
Polymorphism
Proto-oncogene
Reference proteome
Repeat
Repressor
Transcription
Transcription regulation
Ubl conjugation - Interacts With:
- P41279; O00255-2; P19838; Q04864; Q01201; P63165