Names & Taxonomy
- Uniprot ID:
- P55786
- Entry Name:
- PSA_HUMAN
- Status:
- reviewed
- Protein Names:
- Puromycin-sensitive aminopeptidase (PSA) (EC 3.4.11.14) (Cytosol alanyl aminopeptidase) (AAP-S)
- Gene Names:
- NPEPPS PSA
- Gene Names Primary:
- NPEPPS
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 919
- Sequence:
- MWLAAAAPSLARRLLFLGPPPPPLLLLVFSRSSRRRLHSLGLAAMPEKRPFERLPADVSPINYSLCLKPDLLDFTFEGKLEAAAQVRQATNQIVMNCADIDIITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSTLQTGTGTLKIDFVGELNDKMKGFYRSKYTTPSGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISLVVPKDRVALSNMNVIDRKPYPDDENLVEVKFARTPVMSTYLVAFVVGEYDFVETRSKDGVCVRVYTPVGKAEQGKFALEVAAKTLPFYKDYFNVPYPLPKIDLIAIADFAAGAMENWGLVTYRETALLIDPKNSCSSSRQWVALVVGHELAHQWFGNLVTMEWWTHLWLNEGFASWIEYLCVDHCFPEYDIWTQFVSADYTRAQELDALDNSHPIEVSVGHPSEVDEIFDAISYSKGASVIRMLHDYIGDKDFKKGMNMYLTKFQQKNAATEDLWESLENASGKPIAAVMNTWTKQMGFPLIYVEAEQVEDDRLLRLSQKKFCAGGSYVGEDCPQWMVPITISTSEDPNQAKLKILMDKPEMNVVLKNVKPDQWVKLNLGTVGFYRTQYSSAMLESLLPGIRDLSLPPVDRLGLQNDLFSLARAGIISTVEVLKVMEAFVNEPNYTVWSDLSCNLGILSTLLSHTDFYEEIQEFVKDVFSPIGERLGWDPKPGEGHLDALLRGLVLGKLGKAGHKATLEEARRRFKDHVEGKQILSADLRSPVYLTVLKHGDGTTLDIMLKLHKQADMQEEKNRIERVLGATLLPDLIQKVLTFALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELYNRYQGGFLISRLIKLSVEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDAESIHQYLLQRKASPPTV
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm, cytosol
Function
- Function:
- Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain.
- Catalytic Activity:
- Release of an N-terminal amino acid, preferentially alanine, from a wide range of peptides, amides and arylamides.
- Cofactor:
- COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.20 mM for Lys-p-NA
- Enzyme Regulation:
- ENZYME REGULATION: Strongly inhibited by bestatin, leuhistin, actinonin, amastatin, 1,10-phenanthroline, DFP, PCMBS, Zn(2+), Cd(2+), Co(2+), Cu(2+), Hg(2+), EDTA and puromycin. Not inhibited by PMSF, and only slightly inhibited by leupeptin and aprotinin. Activity is increased by Mg(2+) and Ca(2+).
- Active Site:
- ACT_SITE 353 353 Proton acceptor.
- Gene Ontology Go:
- cytosol
extracellular exosome
nucleus
plasma membrane
aminopeptidase activity
metalloaminopeptidase activity
peptide binding
zinc ion binding
antigen processing and presentation of peptide antigen via MHC class I
cellular response to hypoxia
peptide catabolic process
positive regulation of protein targeting to mitochondrion
protein polyubiquitination - Gene Ontology Biological Process:
- antigen processing and presentation of peptide antigen via MHC class I
cellular response to hypoxia
peptide catabolic process
positive regulation of protein targeting to mitochondrion
protein polyubiquitination - Gene Ontology Molecular Function:
- aminopeptidase activity
metalloaminopeptidase activity
peptide binding
zinc ion binding - Gene Ontology Cellular Component:
- cytosol
extracellular exosome
nucleus
plasma membrane - Keywords:
- Alternative splicing
Aminopeptidase
Complete proteome
Cytoplasm
Direct protein sequencing
Hydrolase
Metal-binding
Metalloprotease
Nitration
Nucleus
Protease
Reference proteome
Zinc