Names & Taxonomy
- Uniprot ID:
- P51003
- Entry Name:
- PAPOA_HUMAN
- Status:
- reviewed
- Protein Names:
- Poly(A) polymerase alpha (PAP-alpha) (EC 2.7.7.19) (Polynucleotide adenylyltransferase alpha)
- Gene Names:
- PAPOLA PAP
- Gene Names Primary:
- PAPOLA
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 745
- Sequence:
- MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm. Nucleus. Note=The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic.
Function
- Function:
- Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
- Catalytic Activity:
- ATP + RNA(n) = diphosphate + RNA(n+1).
- Cofactor:
- COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Gene Ontology Go:
- cytoplasm
nucleoplasm
nucleus
ATP binding
magnesium ion binding
manganese ion binding
polynucleotide adenylyltransferase activity
RNA binding
gene expression
mRNA 3'-end processing
mRNA polyadenylation
mRNA splicing, via spliceosome
regulation of mRNA 3'-end processing
RNA polyadenylation
RNA splicing
termination of RNA polymerase II transcription
transcription from RNA polymerase II promoter - Gene Ontology Biological Process:
- gene expression
mRNA 3'-end processing
mRNA polyadenylation
mRNA splicing, via spliceosome
regulation of mRNA 3'-end processing
RNA polyadenylation
RNA splicing
termination of RNA polymerase II transcription
transcription from RNA polymerase II promoter - Gene Ontology Molecular Function:
- ATP binding
magnesium ion binding
manganese ion binding
polynucleotide adenylyltransferase activity
RNA binding - Gene Ontology Cellular Component:
- cytoplasm
nucleoplasm
nucleus - Keywords:
- ATP-binding
Acetylation
Alternative splicing
Complete proteome
Cytoplasm
Isopeptide bond
Magnesium
Manganese
Metal-binding
Nucleotide-binding
Nucleus
Phosphoprotein
RNA-binding
Reference proteome
Transferase
Ubl conjugation
mRNA processing