Names & Taxonomy

Uniprot ID:
P49773
Entry Name:
HINT1_HUMAN
Status:
reviewed
Protein Names:
Histidine triad nucleotide-binding protein 1 (EC 3.-.-.-) (Adenosine 5'-monophosphoramidase) (Protein kinase C inhibitor 1) (Protein kinase C-interacting protein 1) (PKCI-1)
Gene Names:
HINT1 HINT PKCI1 PRKCNH1
Gene Names Primary:
HINT1
Organism:
Homo sapiens (Human)

Structure

Length:
126
Sequence:
MADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVAEDDDESLLGHLMIVGKKCAADLGLNKGYRMVVNEGSDGGQSVYHVHLHVLGGRQMHWPPG
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm. Nucleus. Note=Interaction with CDK7 leads to a more nuclear localization.

Function

Function:
Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex.
Active Site:
ACT_SITE 112 112 Tele-AMP-histidine intermediate.
Cross Reference Drug Bank:
DB00131
Gene Ontology Go:
cytoplasm
cytoskeleton
extracellular exosome
histone deacetylase complex
nucleus
plasma membrane
hydrolase activity
nucleotide binding
protein kinase C binding
intrinsic apoptotic signaling pathway by p53 class mediator
positive regulation of calcium-mediated signaling
purine ribonucleotide catabolic process
regulation of transcription, DNA-templated
signal transduction
transcription, DNA-templated
Gene Ontology Biological Process:
intrinsic apoptotic signaling pathway by p53 class mediator
positive regulation of calcium-mediated signaling
purine ribonucleotide catabolic process
regulation of transcription, DNA-templated
signal transduction
transcription, DNA-templated
Gene Ontology Molecular Function:
hydrolase activity
nucleotide binding
protein kinase C binding
Gene Ontology Cellular Component:
cytoplasm
cytoskeleton
extracellular exosome
histone deacetylase complex
nucleus
plasma membrane
Keywords:
3D-structure
Acetylation
Apoptosis
Complete proteome
Cytoplasm
Direct protein sequencing
Disease mutation
Hydrolase
Neuropathy
Nucleotide-binding
Nucleus
Phosphoprotein
Reference proteome
Transcription
Transcription regulation

Publication

PubMed ID:
8812426 7644499 14702039 15489334 8643579 12665801 15703176 16014379 16835243 17337452 19112177 19608861 21269460 22647378 22814378 24275569 9323207 22869114 22329685 22961002