Names & Taxonomy
- Uniprot ID:
- P47712
- Entry Name:
- PA24A_HUMAN
- Status:
- reviewed
- Protein Names:
- Cytosolic phospholipase A2 (cPLA2) (Phospholipase A2 group IVA) [Includes: Phospholipase A2 (EC 3.1.1.4) (Phosphatidylcholine 2-acylhydrolase); Lysophospholipase (EC 3.1.1.5)]
- Gene Names:
- PLA2G4A CPLA2 PLA2G4
- Gene Names Primary:
- PLA2G4A
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 749
- Sequence:
- MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSMKVGEKKEVPFIFNQVTEMVLEMSLEVCSCPDLRFSMALCDQEKTFRQQRKEHIRESMKKLLGPKNSEGLHSARDVPVVAILGSGGGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFSPYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSRGSTMEEELENITTKHIVSNDSSDSDDESHEPKGTENEDAGSDYQSDNQASWIHRMIMALVSDSALFNTREGRAGKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEKDCPTIIHFVLANINFRKYRAPGVPRETEEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMHFNTLNNIDVIKEAMVESIEYRRQNPSRCSVSLSNVEARRFFNKEFLSKPKA
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm. Cytoplasmic vesicle. Note=Translocates to membrane vesicles in a calcium-dependent fashion.
Function
- Function:
- Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
- Catalytic Activity:
- Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.; 2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate.
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: Vmax=2.7 umol/min/mg enzyme for the phospholipase A2 reaction
- Enzyme Regulation:
- ENZYME REGULATION: Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca(2+).
- Active Site:
- ACT_SITE 228 228 Nucleophile.; ACT_SITE 549 549 Proton acceptor.
- Cross Reference Drug Bank:
- DB00041 DB00411 DB00578 DB00445 DB00591 DB00588 DB04552 DB01083 DB01103 DB00086 DB04786
- Gene Ontology Go:
- cytoplasm
cytosol
endoplasmic reticulum membrane
Golgi apparatus
mitochondrial inner membrane
nucleus
perinuclear region of cytoplasm
zymogen granule
calcium ion binding
calcium-dependent phospholipase A2 activity
calcium-dependent phospholipid binding
lysophospholipase activity
phospholipase A2 activity
aging
arachidonic acid metabolic process
arachidonic acid secretion
blood coagulation
cardiolipin acyl-chain remodeling
cellular response to antibiotic
decidualization
glycerophospholipid biosynthetic process
icosanoid biosynthetic process
icosanoid metabolic process
luteolysis
ovulation from ovarian follicle
phosphatidic acid biosynthetic process
phosphatidylcholine acyl-chain remodeling
phosphatidylethanolamine acyl-chain remodeling
phosphatidylglycerol acyl-chain remodeling
phosphatidylinositol acyl-chain remodeling
phosphatidylserine acyl-chain remodeling
phospholipid catabolic process
phospholipid metabolic process
platelet activating factor biosynthetic process
platelet activation
positive regulation of apoptotic process
positive regulation of bone mineralization
positive regulation of cell proliferation
positive regulation of fever generation
positive regulation of prostaglandin biosynthetic process
positive regulation of vesicle fusion
response to calcium ion
response to glucocorticoid
response to heat
response to hydrogen peroxide
response to lipopolysaccharide
response to lithium ion
response to methylmercury
response to organonitrogen compound
response to vitamin D
small molecule metabolic process
surfactant homeostasis - Gene Ontology Biological Process:
- aging
arachidonic acid metabolic process
arachidonic acid secretion
blood coagulation
cardiolipin acyl-chain remodeling
cellular response to antibiotic
decidualization
glycerophospholipid biosynthetic process
icosanoid biosynthetic process
icosanoid metabolic process
luteolysis
ovulation from ovarian follicle
phosphatidic acid biosynthetic process
phosphatidylcholine acyl-chain remodeling
phosphatidylethanolamine acyl-chain remodeling
phosphatidylglycerol acyl-chain remodeling
phosphatidylinositol acyl-chain remodeling
phosphatidylserine acyl-chain remodeling
phospholipid catabolic process
phospholipid metabolic process
platelet activating factor biosynthetic process
platelet activation
positive regulation of apoptotic process
positive regulation of bone mineralization
positive regulation of cell proliferation
positive regulation of fever generation
positive regulation of prostaglandin biosynthetic process
positive regulation of vesicle fusion
response to calcium ion
response to glucocorticoid
response to heat
response to hydrogen peroxide
response to lipopolysaccharide
response to lithium ion
response to methylmercury
response to organonitrogen compound
response to vitamin D
small molecule metabolic process
surfactant homeostasis - Gene Ontology Molecular Function:
- calcium-dependent phospholipase A2 activity
calcium-dependent phospholipid binding
calcium ion binding
lysophospholipase activity
phospholipase A2 activity - Gene Ontology Cellular Component:
- cytoplasm
cytosol
endoplasmic reticulum membrane
Golgi apparatus
mitochondrial inner membrane
nucleus
perinuclear region of cytoplasm
zymogen granule - Keywords:
- 3D-structure
Calcium
Complete proteome
Cytoplasm
Cytoplasmic vesicle
Direct protein sequencing
Disease mutation
Hydrolase
Lipid degradation
Lipid metabolism
Metal-binding
Phosphoprotein
Polymorphism
Reference proteome