Names & Taxonomy

Uniprot ID:
P32322
Entry Name:
P5CR1_HUMAN
Status:
reviewed
Protein Names:
Pyrroline-5-carboxylate reductase 1, mitochondrial (P5C reductase 1) (P5CR 1) (EC 1.5.1.2)
Gene Names:
PYCR1
Gene Names Primary:
PYCR1
Organism:
Homo sapiens (Human)

Structure

Length:
319
Sequence:
MSVGFIGAGQLAFALAKGFTAAGVLAAHKIMASSPDMDLATVSALRKMGVKLTPHNKETVQHSDVLFLAVKPHIIPFILDEIGADIEDRHIVVSCAAGVTISSIEKKLSAFRPAPRVIRCMTNTPVVVREGATVYATGTHAQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEASCIRTRELQSMADQEQVSPAAIKKTILDKVKLDSPAGTALSPSGHTKLLPRSLAPAGKD
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Mitochondrion

Function

Function:
Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress.
Pathway:
Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
Catalytic Activity:
L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H.
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.151 mM for NAD(+)
Enzyme Regulation:
ENZYME REGULATION: Subject to competitive inhibition by the reaction product proline. Subject to competitive inhibition by stearoyl coenzyme A.
Cross Reference Drug Bank:
DB00172
Gene Ontology Go:
mitochondrial matrix
mitochondrion
identical protein binding
pyrroline-5-carboxylate reductase activity
cellular amino acid biosynthetic process
cellular nitrogen compound metabolic process
cellular response to oxidative stress
L-proline biosynthetic process
negative regulation of hydrogen peroxide-induced cell death
proline biosynthetic process
regulation of mitochondrial membrane potential
small molecule metabolic process
Gene Ontology Biological Process:
cellular amino acid biosynthetic process
cellular nitrogen compound metabolic process
cellular response to oxidative stress
L-proline biosynthetic process
negative regulation of hydrogen peroxide-induced cell death
proline biosynthetic process
regulation of mitochondrial membrane potential
small molecule metabolic process
Gene Ontology Molecular Function:
identical protein binding
pyrroline-5-carboxylate reductase activity
Gene Ontology Cellular Component:
mitochondrial matrix
mitochondrion
Keywords:
3D-structure
Acetylation
Alternative splicing
Amino-acid biosynthesis
Complete proteome
Direct protein sequencing
Disease mutation
Mitochondrion
NADP
Oxidoreductase
Polymorphism
Proline biosynthesis
Reference proteome
Stress response
Interacts With:
Itself; Q99497

Publication

PubMed ID:
1730675 14702039 16625196 15489334 19413330 19648921 20068231 21269460 25944712 16730026 19576563 22052856