Names & Taxonomy
- Uniprot ID:
- P32322
- Entry Name:
- P5CR1_HUMAN
- Status:
- reviewed
- Protein Names:
- Pyrroline-5-carboxylate reductase 1, mitochondrial (P5C reductase 1) (P5CR 1) (EC 1.5.1.2)
- Gene Names:
- PYCR1
- Gene Names Primary:
- PYCR1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 319
- Sequence:
- MSVGFIGAGQLAFALAKGFTAAGVLAAHKIMASSPDMDLATVSALRKMGVKLTPHNKETVQHSDVLFLAVKPHIIPFILDEIGADIEDRHIVVSCAAGVTISSIEKKLSAFRPAPRVIRCMTNTPVVVREGATVYATGTHAQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEASCIRTRELQSMADQEQVSPAAIKKTILDKVKLDSPAGTALSPSGHTKLLPRSLAPAGKD
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Mitochondrion
Function
- Function:
- Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress.
- Pathway:
- Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
- Catalytic Activity:
- L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H.
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.151 mM for NAD(+)
- Enzyme Regulation:
- ENZYME REGULATION: Subject to competitive inhibition by the reaction product proline. Subject to competitive inhibition by stearoyl coenzyme A.
- Cross Reference Drug Bank:
- DB00172
- Gene Ontology Go:
- mitochondrial matrix
mitochondrion
identical protein binding
pyrroline-5-carboxylate reductase activity
cellular amino acid biosynthetic process
cellular nitrogen compound metabolic process
cellular response to oxidative stress
L-proline biosynthetic process
negative regulation of hydrogen peroxide-induced cell death
proline biosynthetic process
regulation of mitochondrial membrane potential
small molecule metabolic process - Gene Ontology Biological Process:
- cellular amino acid biosynthetic process
cellular nitrogen compound metabolic process
cellular response to oxidative stress
L-proline biosynthetic process
negative regulation of hydrogen peroxide-induced cell death
proline biosynthetic process
regulation of mitochondrial membrane potential
small molecule metabolic process - Gene Ontology Molecular Function:
- identical protein binding
pyrroline-5-carboxylate reductase activity - Gene Ontology Cellular Component:
- mitochondrial matrix
mitochondrion - Keywords:
- 3D-structure
Acetylation
Alternative splicing
Amino-acid biosynthesis
Complete proteome
Direct protein sequencing
Disease mutation
Mitochondrion
NADP
Oxidoreductase
Polymorphism
Proline biosynthesis
Reference proteome
Stress response - Interacts With:
- Itself; Q99497