Names & Taxonomy
- Uniprot ID:
- P31513
- Entry Name:
- FMO3_HUMAN
- Status:
- reviewed
- Protein Names:
- Dimethylaniline monooxygenase [N-oxide-forming] 3 (EC 1.14.13.8) (Dimethylaniline oxidase 3) (FMO II) (FMO form 2) (Hepatic flavin-containing monooxygenase 3) (FMO 3) (Trimethylamine monooxygenase) (EC 1.14.13.148)
- Gene Names:
- FMO3
- Gene Names Primary:
- FMO3
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 532
- Sequence:
- MGKKVAIIGAGVSGLASIRSCLEEGLEPTCFEKSNDIGGLWKFSDHAEEGRASIYKSVFSNSSKEMMCFPDFPFPDDFPNFMHNSKIQEYIIAFAKEKNLLKYIQFKTFVSSVNKHPDFATTGQWDVTTERDGKKESAVFDAVMVCSGHHVYPNLPKESFPGLNHFKGKCFHSRDYKEPGVFNGKRVLVVGLGNSGCDIATELSRTAEQVMISSRSGSWVMSRVWDNGYPWDMLLVTRFGTFLKNNLPTAISDWLYVKQMNARFKHENYGLMPLNGVLRKEPVFNDELPASILCGIVSVKPNVKEFTETSAIFEDGTIFEGIDCVIFATGYSFAYPFLDESIIKSRNNEIILFKGVFPPLLEKSTIAVIGFVQSLGAAIPTVDLQSRWAAQVIKGTCTLPSMEDMMNDINEKMEKKRKWFGKSETIQTDYIVYMDELSSFIGAKPNIPWLFLTDPKLAMEVYFGPCSPYQFRLVGPGQWPGARNAILTQWDRSLKPMQTRVVGRLQKPCFFFHWLKLFAIPILLIAVFLVLT
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Microsome membrane. Endoplasmic reticulum membrane.
Function
- Function:
- Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds (PubMed:9224773).
- Catalytic Activity:
- N,N-dimethylaniline + NADPH + O(2) = N,N-dimethylaniline N-oxide + NADP(+) + H(2)O.
- Cofactor:
- COFACTOR: Name=FAD; Xref=ChEBI:CHEBI:57692;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=21 uM for trimethylamine (at pH 8.5)
- Cross Reference Drug Bank:
- DB00918 DB00501 DB00363 DB00250 DB01254 DB00334 DB00675 DB05294 DB00582
- Gene Ontology Go:
- endoplasmic reticulum membrane
integral component of membrane
intracellular membrane-bounded organelle
amino acid binding
flavin adenine dinucleotide binding
monooxygenase activity
N,N-dimethylaniline monooxygenase activity
NADP binding
trimethylamine monooxygenase activity
drug metabolic process
oxidation-reduction process
small molecule metabolic process
xenobiotic metabolic process - Gene Ontology Biological Process:
- drug metabolic process
oxidation-reduction process
small molecule metabolic process
xenobiotic metabolic process - Gene Ontology Molecular Function:
- amino acid binding
flavin adenine dinucleotide binding
monooxygenase activity
N,N-dimethylaniline monooxygenase activity
NADP binding
trimethylamine monooxygenase activity - Gene Ontology Cellular Component:
- endoplasmic reticulum membrane
integral component of membrane
intracellular membrane-bounded organelle - Keywords:
- Complete proteome
Direct protein sequencing
Disease mutation
Endoplasmic reticulum
FAD
Flavoprotein
Membrane
Microsome
Monooxygenase
NADP
Oxidoreductase
Phosphoprotein
Polymorphism
Reference proteome
Transmembrane