Names & Taxonomy
- Uniprot ID:
- P29475
- Entry Name:
- NOS1_HUMAN
- Status:
- reviewed
- Protein Names:
- Nitric oxide synthase, brain (EC 1.14.13.39) (Constitutive NOS) (NC-NOS) (NOS type I) (Neuronal NOS) (N-NOS) (nNOS) (Peptidyl-cysteine S-nitrosylase NOS1) (bNOS)
- Gene Names:
- NOS1
- Gene Names Primary:
- NOS1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 1434
- Sequence:
- MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine
Function
- Function:
- Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
- Catalytic Activity:
- 2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
- Cofactor:
- COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413; ; COFACTOR: Name=FAD; Xref=ChEBI:CHEBI:57692; ; Note=Binds 1 FAD.;; COFACTOR: Name=FMN; Xref=ChEBI:CHEBI:58210; ; Note=Binds 1 FMN.;; COFACTOR: Name=5,6,7,8-tetrahydrobiopterin; Xref=ChEBI:CHEBI:15372; ; Note=Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.;
- Enzyme Regulation:
- ENZYME REGULATION: Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP.
- Cross Reference Drug Bank:
- DB00997 DB03147 DB00155
- Gene Ontology Go:
- azurophil granule
caveola
cytoplasm
cytoskeleton
cytosol
dendritic spine
membrane raft
mitochondrial outer membrane
mitochondrion
nuclear membrane
perinuclear region of cytoplasm
photoreceptor inner segment
postsynaptic density
protein complex
sarcolemma
sarcoplasmic reticulum
sarcoplasmic reticulum membrane
synapse
T-tubule
vesicle membrane
Z disc
arginine binding
cadmium ion binding
flavin adenine dinucleotide binding
FMN binding
heme binding
ion channel binding
iron ion binding
NADP binding
nitric-oxide synthase activity
scaffold protein binding
sodium channel regulator activity
tetrahydrobiopterin binding
aging
arginine catabolic process
behavioral response to cocaine
blood coagulation
cardiac conduction
cell redox homeostasis
cellular response to growth factor stimulus
cellular response to mechanical stimulus
exogenous drug catabolic process
female pregnancy
multicellular organismal response to stress
myoblast fusion
negative regulation of adrenergic receptor signaling pathway involved in heart process
negative regulation of apoptotic process
negative regulation of blood pressure
negative regulation of calcium ion transport
negative regulation of calcium ion transport into cytosol
negative regulation of cell proliferation
negative regulation of cytosolic calcium ion concentration
negative regulation of heart contraction
negative regulation of hydrolase activity
negative regulation of insulin secretion
negative regulation of potassium ion transport
negative regulation of serotonin uptake
negative regulation of vasoconstriction
neurotransmitter biosynthetic process
nitric oxide biosynthetic process
nitric oxide mediated signal transduction
peptidyl-cysteine S-nitrosylation
positive regulation of adrenergic receptor signaling pathway involved in heart process
positive regulation of guanylate cyclase activity
positive regulation of histone acetylation
positive regulation of long-term synaptic potentiation
positive regulation of neuron death
positive regulation of sodium ion transmembrane transport
positive regulation of the force of heart contraction
positive regulation of transcription from RNA polymerase II promoter
positive regulation of transcription, DNA-templated
positive regulation of vasodilation
regulation of cardiac conduction
regulation of cardiac muscle contraction
regulation of generation of L-type calcium current
regulation of neurogenesis
regulation of ryanodine-sensitive calcium-release channel activity
regulation of sensory perception of pain
regulation of sodium ion transport
response to activity
response to estrogen
response to ethanol
response to heat
response to hypoxia
response to lead ion
response to lipopolysaccharide
response to nicotine
response to nitric oxide
response to peptide hormone
response to vitamin E
striated muscle contraction - Gene Ontology Biological Process:
- aging
arginine catabolic process
behavioral response to cocaine
blood coagulation
cardiac conduction
cell redox homeostasis
cellular response to growth factor stimulus
cellular response to mechanical stimulus
exogenous drug catabolic process
female pregnancy
multicellular organismal response to stress
myoblast fusion
negative regulation of adrenergic receptor signaling pathway involved in heart process
negative regulation of apoptotic process
negative regulation of blood pressure
negative regulation of calcium ion transport
negative regulation of calcium ion transport into cytosol
negative regulation of cell proliferation
negative regulation of cytosolic calcium ion concentration
negative regulation of heart contraction
negative regulation of hydrolase activity
negative regulation of insulin secretion
negative regulation of potassium ion transport
negative regulation of serotonin uptake
negative regulation of vasoconstriction
neurotransmitter biosynthetic process
nitric oxide biosynthetic process
nitric oxide mediated signal transduction
peptidyl-cysteine S-nitrosylation
positive regulation of adrenergic receptor signaling pathway involved in heart process
positive regulation of guanylate cyclase activity
positive regulation of histone acetylation
positive regulation of long-term synaptic potentiation
positive regulation of neuron death
positive regulation of sodium ion transmembrane transport
positive regulation of the force of heart contraction
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of vasodilation
regulation of cardiac conduction
regulation of cardiac muscle contraction
regulation of generation of L-type calcium current
regulation of neurogenesis
regulation of ryanodine-sensitive calcium-release channel activity
regulation of sensory perception of pain
regulation of sodium ion transport
response to activity
response to estrogen
response to ethanol
response to heat
response to hypoxia
response to lead ion
response to lipopolysaccharide
response to nicotine
response to nitric oxide
response to peptide hormone
response to vitamin E
striated muscle contraction - Gene Ontology Molecular Function:
- arginine binding
cadmium ion binding
flavin adenine dinucleotide binding
FMN binding
heme binding
ion channel binding
iron ion binding
NADP binding
nitric-oxide synthase activity
scaffold protein binding
sodium channel regulator activity
tetrahydrobiopterin binding - Gene Ontology Cellular Component:
- azurophil granule
caveola
cytoplasm
cytoskeleton
cytosol
dendritic spine
membrane raft
mitochondrial outer membrane
mitochondrion
nuclear membrane
perinuclear region of cytoplasm
photoreceptor inner segment
postsynaptic density
protein complex
sarcolemma
sarcoplasmic reticulum
sarcoplasmic reticulum membrane
synapse
T-tubule
vesicle membrane
Z disc - Keywords:
- 3D-structure
Alternative splicing
Calmodulin-binding
Cell membrane
Cell projection
Complete proteome
FAD
FMN
Flavoprotein
Heme
Iron
Membrane
Metal-binding
NADP
Oxidoreductase
Phosphoprotein
Polymorphism
Reference proteome
Ubl conjugation - Interacts With:
- Q08AM6