Names & Taxonomy

Uniprot ID:
P28062
Entry Name:
PSB8_HUMAN
Status:
reviewed
Protein Names:
Proteasome subunit beta type-8 (EC 3.4.25.1) (Low molecular mass protein 7) (Macropain subunit C13) (Multicatalytic endopeptidase complex subunit C13) (Proteasome component C13) (Proteasome subunit beta-5i) (Really interesting new gene 10 protein)
Gene Names:
PSMB8 LMP7 PSMB5i RING10 Y2
Gene Names Primary:
PSMB8
Organism:
Homo sapiens (Human)

Structure

Length:
276
Sequence:
MALLDVCGAPRGQRPESALPVAGSGRRSDPGHYSFSMRSPELALPRGMQPTEFFQSLGGDGERNVQIEMAHGTTTLAFKFQHGVIAAVDSRASAGSYISALRVNKVIEINPYLLGTMSGCAADCQYWERLLAKECRLYYLRNGERISVSAASKLLSNMMCQYRGMGLSMGSMICGWDKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPNLSPEEAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm

Function

Function:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
Catalytic Activity:
Cleavage of peptide bonds with very broad specificity.
Active Site:
ACT_SITE 73 73 Nucleophile.
Cross Reference Drug Bank:
DB08889
Gene Ontology Go:
cytosol
extracellular exosome
nucleoplasm
proteasome complex
proteasome core complex
spermatoproteasome complex
threonine-type endopeptidase activity
activation of MAPKK activity
anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
antigen processing and presentation of exogenous peptide antigen via MHC class I
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
antigen processing and presentation of peptide antigen via MHC class I
apoptotic process
axon guidance
cellular nitrogen compound metabolic process
cytokine-mediated signaling pathway
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
epidermal growth factor receptor signaling pathway
fat cell differentiation
Fc-epsilon receptor signaling pathway
fibroblast growth factor receptor signaling pathway
G1/S transition of mitotic cell cycle
gene expression
innate immune response
insulin receptor signaling pathway
MAPK cascade
mitotic cell cycle
negative regulation of apoptotic process
negative regulation of canonical Wnt signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
neurotrophin TRK receptor signaling pathway
NIK/NF-kappaB signaling
polyamine metabolic process
positive regulation of canonical Wnt signaling pathway
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
programmed cell death
protein polyubiquitination
Ras protein signal transduction
regulation of apoptotic process
regulation of cellular amino acid metabolic process
regulation of endopeptidase activity
regulation of mRNA stability
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
small GTPase mediated signal transduction
small molecule metabolic process
stimulatory C-type lectin receptor signaling pathway
T cell receptor signaling pathway
tumor necrosis factor-mediated signaling pathway
type I interferon signaling pathway
vascular endothelial growth factor receptor signaling pathway
viral process
Gene Ontology Biological Process:
activation of MAPKK activity
anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
antigen processing and presentation of exogenous peptide antigen via MHC class I
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
antigen processing and presentation of peptide antigen via MHC class I
apoptotic process
axon guidance
cellular nitrogen compound metabolic process
cytokine-mediated signaling pathway
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
epidermal growth factor receptor signaling pathway
fat cell differentiation
Fc-epsilon receptor signaling pathway
fibroblast growth factor receptor signaling pathway
G1/S transition of mitotic cell cycle
gene expression
innate immune response
insulin receptor signaling pathway
MAPK cascade
mitotic cell cycle
negative regulation of apoptotic process
negative regulation of canonical Wnt signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
neurotrophin TRK receptor signaling pathway
NIK/NF-kappaB signaling
polyamine metabolic process
positive regulation of canonical Wnt signaling pathway
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
programmed cell death
protein polyubiquitination
Ras protein signal transduction
regulation of apoptotic process
regulation of cellular amino acid metabolic process
regulation of endopeptidase activity
regulation of mRNA stability
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
small GTPase mediated signal transduction
small molecule metabolic process
stimulatory C-type lectin receptor signaling pathway
T cell receptor signaling pathway
tumor necrosis factor-mediated signaling pathway
type I interferon signaling pathway
vascular endothelial growth factor receptor signaling pathway
viral process
Gene Ontology Molecular Function:
threonine-type endopeptidase activity
Gene Ontology Cellular Component:
cytosol
extracellular exosome
nucleoplasm
proteasome complex
proteasome core complex
spermatoproteasome complex
Keywords:
Alternative splicing
Complete proteome
Cytoplasm
Differentiation
Disease mutation
Host-virus interaction
Hydrolase
Immunity
Nucleus
Phosphoprotein
Polymorphism
Protease
Proteasome
Reference proteome
Threonine protease
Zymogen
Interacts With:
P27958

Publication

PubMed ID:
8458375 1453454 1922342 1429565 8344725 8568858 14574404 15489334 9157092 8163024 8663318 11493458 11717192 14550573 15501285 15907481 15488952 15944226 16423992 17142736 17262812 18669648 19443843 19619915 19525961 21269460 21881205 24275569 25944712 21129723 21953331 21852578 26524591 26567544