Names & Taxonomy
- Uniprot ID:
- P27815
- Entry Name:
- PDE4A_HUMAN
- Status:
- reviewed
- Protein Names:
- cAMP-specific 3',5'-cyclic phosphodiesterase 4A (EC 3.1.4.53) (DPDE2) (PDE46)
- Gene Names:
- PDE4A DPDE2
- Gene Names Primary:
- PDE4A
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 886
- Sequence:
- MEPPTVPSERSLSLSLPGPREGQATLKPPPQHLWRQPRTPIRIQQRGYSDSAERAERERQPHRPIERADAMDTSDRPGLRTTRMSWPSSFHGTGTGSGGAGGGSSRRFEAENGPTPSPGRSPLDSQASPGLVLHAGAATSQRRESFLYRSDSDYDMSPKTMSRNSSVTSEAHAEDLIVTPFAQVLASLRSVRSNFSLLTNVPVPSNKRSPLGGPTPVCKATLSEETCQQLARETLEELDWCLEQLETMQTYRSVSEMASHKFKRMLNRELTHLSEMSRSGNQVSEYISTTFLDKQNEVEIPSPTMKEREKQQAPRPRPSQPPPPPVPHLQPMSQITGLKKLMHSNSLNNSNIPRFGVKTDQEELLAQELENLNKWGLNIFCVSDYAGGRSLTCIMYMIFQERDLLKKFRIPVDTMVTYMLTLEDHYHADVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQSPSPPPEEESRGPGHPPLPDKFQFELTLEEEEEEEISMAQIPCTAQEALTAQGLSGVEEALDATIAWEASPAQESLEVMAQEASLEAELEAVYLTQQAQSTGSAPVAPDEFSSREEFVVAVSHSSPSALALQSPLLPAWRTLSVSEHAPGLPGLPSTAAEVEAQREHQAAKRACSACAGTFGEDTSALPAPGGGGSGGDPT
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Isoform 1: Cytoplasm, perinuclear region.; Isoform 2: Cytoplasm, perinuclear region. Cell projection, ruffle membrane.; Isoform 4: Membrane; Peripheral membrane protein. Note=Isoform 4 has propensity for association with membranes.; Isoform 6: Cytoplasm, perinuclear region.; Isoform 7: Cytoplasm. Membrane. Note=Predominantly cytoplasmic.
Function
- Function:
- Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
- Pathway:
- Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
- Catalytic Activity:
- Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate.
- Cofactor:
- COFACTOR: Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=4.0 uM for cAMP (isoform 2)
- Enzyme Regulation:
- ENZYME REGULATION: Isoform 1, isoform 2, isoform 6 and isoform 7 are inhibited by rolipram and cilomilast. Isoform 1, isoform 2 and isoform 6 are inhibited by 4--2-imidazolidinone (Ro 20-1724), roflumilast and denbufylline.
- Active Site:
- ACT_SITE 433 433 Proton donor.
- Cross Reference Drug Bank:
- DB05676 DB00201 DB00975 DB06751 DB00651 DB00824 DB05266 DB01088 DB00920 DB01303 DB00806 DB01656 DB00277 DB08811
- Gene Ontology Go:
- cytosol
membrane
perinuclear region of cytoplasm
ruffle membrane
3',5'-cyclic-AMP phosphodiesterase activity
cAMP binding
metal ion binding
cAMP catabolic process
cellular response to drug
regulation of cAMP-mediated signaling
regulation of protein kinase A signaling
sensory perception of smell
signal transduction - Gene Ontology Biological Process:
- cAMP catabolic process
cellular response to drug
regulation of cAMP-mediated signaling
regulation of protein kinase A signaling
sensory perception of smell
signal transduction - Gene Ontology Molecular Function:
- 3',5'-cyclic-AMP phosphodiesterase activity
cAMP binding
metal ion binding - Gene Ontology Cellular Component:
- cytosol
membrane
perinuclear region of cytoplasm
ruffle membrane - Keywords:
- 3D-structure
Alternative splicing
Cell membrane
Cell projection
Complete proteome
Cytoplasm
Hydrolase
Isopeptide bond
Membrane
Metal-binding
Phosphoprotein
Polymorphism
Reference proteome
Ubl conjugation
cAMP