Names & Taxonomy

Uniprot ID:
P26572
Entry Name:
MGAT1_HUMAN
Status:
reviewed
Protein Names:
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC 2.4.1.101) (N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I) (GNT-I) (GlcNAc-T I)
Gene Names:
MGAT1 GGNT1 GLCT1 GLYT1 MGAT
Gene Names Primary:
MGAT1
Organism:
Homo sapiens (Human)

Structure

Length:
445
Sequence:
MLKKQSAGLVLWGAILFVAWNALLLLFFWTRPAPGRPPSVSALDGDPASLTREVIRLAQDAEVELERQRGLLQQIGDALSSQRGRVPTAAPPAQPRVPVTPAPAVIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDCGHEETAQAIASYGSAVTHIRQPDLSSIAVPPDHRKFQGYYKIARHYRWALGQVFRQFRFPAAVVVEDDLEVAPDFFEYFRATYPLLKADPSLWCVSAWNDNGKEQMVDASRPELLYRTDFFPGLGWLLLAELWAELEPKWPKAFWDDWMRRPEQRQGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQFVHFTQLDLSYLQREAYDRDFLARVYGAPQLQVEKVRTNDRKELGEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAPPLTWEGYDPSWN
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Golgi apparatus membrane; Single-pass type II membrane protein.

Function

Function:
Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
Pathway:
Protein modification; protein glycosylation.
Catalytic Activity:
UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor:
COFACTOR: Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
Active Site:
ACT_SITE 289 289 Proton acceptor.
Gene Ontology Go:
extracellular exosome
extracellular vesicle
Golgi membrane
integral component of membrane
membrane
acetylglucosaminyltransferase activity
alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity
metal ion binding
carbohydrate metabolic process
cellular protein metabolic process
in utero embryonic development
post-translational protein modification
protein glycosylation
protein N-linked glycosylation via asparagine
UDP-N-acetylglucosamine catabolic process
Gene Ontology Biological Process:
carbohydrate metabolic process
cellular protein metabolic process
in utero embryonic development
post-translational protein modification
protein glycosylation
protein N-linked glycosylation via asparagine
UDP-N-acetylglucosamine catabolic process
Gene Ontology Molecular Function:
acetylglucosaminyltransferase activity
alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity
metal ion binding
Gene Ontology Cellular Component:
extracellular exosome
extracellular vesicle
Golgi membrane
integral component of membrane
membrane
Keywords:
Complete proteome
Disulfide bond
Glycosyltransferase
Golgi apparatus
Manganese
Membrane
Metal-binding
Polymorphism
Reference proteome
Signal-anchor
Transferase
Transmembrane
Transmembrane helix

Publication

PubMed ID:
1702225 1827260 14702039 15372022 15489334 24275569