Names & Taxonomy
- Uniprot ID:
- P24928
- Entry Name:
- RPB1_HUMAN
- Status:
- reviewed
- Protein Names:
- DNA-directed RNA polymerase II subunit RPB1 (RNA polymerase II subunit B1) (EC 2.7.7.6) (DNA-directed RNA polymerase II subunit A) (DNA-directed RNA polymerase III largest subunit) (RNA-directed RNA polymerase II subunit RPB1) (EC 2.7.7.48)
- Gene Names:
- POLR2A POLR2
- Gene Names Primary:
- POLR2A
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 1970
- Sequence:
- MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPASPKYTPTSPSYSPSSPEYTPTSPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Nucleus
Function
- Function:
- DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
- Catalytic Activity:
- Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
- Gene Ontology Go:
- DNA-directed RNA polymerase II, core complex
nucleoplasm
nucleus
DNA binding
DNA-directed RNA polymerase activity
metal ion binding
poly(A) RNA binding
RNA-directed RNA polymerase activity
ubiquitin protein ligase binding
7-methylguanosine mRNA capping
DNA repair
DNA-templated transcription, termination
gene expression
gene silencing by RNA
mRNA splicing, via spliceosome
nucleotide-excision repair
piRNA metabolic process
positive regulation of RNA splicing
positive regulation of viral transcription
regulation of transcription, DNA-templated
RNA splicing
somatic stem cell population maintenance
transcription elongation from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription-coupled nucleotide-excision repair
viral process - Gene Ontology Biological Process:
- 7-methylguanosine mRNA capping
DNA repair
DNA-templated transcription, termination
gene expression
gene silencing by RNA
mRNA splicing, via spliceosome
nucleotide-excision repair
piRNA metabolic process
positive regulation of RNA splicing
positive regulation of viral transcription
regulation of transcription, DNA-templated
RNA splicing
somatic stem cell population maintenance
transcription-coupled nucleotide-excision repair
transcription elongation from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
viral process - Gene Ontology Molecular Function:
- DNA binding
DNA-directed RNA polymerase activity
metal ion binding
poly(A) RNA binding
RNA-directed RNA polymerase activity
ubiquitin protein ligase binding - Gene Ontology Cellular Component:
- DNA-directed RNA polymerase II, core complex
nucleoplasm
nucleus - Keywords:
- 3D-structure
Acetylation
Alternative splicing
Complete proteome
DNA-binding
DNA-directed RNA polymerase
Magnesium
Metal-binding
Methylation
Nucleotidyltransferase
Nucleus
Phosphoprotein
Polymorphism
RNA-directed RNA polymerase
Reference proteome
Repeat
Transcription
Transferase
Ubl conjugation
Zinc - Interacts With:
- Q6P1J9; P17844; Q67020; Q8N7H5; Q9NQG5; Q96H20; O00267; Q9HCS7
Publication
- PubMed ID:
- 1542581 7622068 16625196 15489334 9852112 9671816 10393184 10454543 12381297 12376575 12527917 15144186 15563843 14684736 14992727 15992770 16118227 16314571 16964243 16491129 17234882 18533109 18550522 18032511 17998332 18669648 19413330 19106100 19450536 19667075 19136461 19690332 20231364 20068231 21269460 21536736 21127351 21406692 21454787 22137580 22466610 23029222 23149945 24656813 24275569 24207025 26687004 26700805 17157258 23748380