Names & Taxonomy

Uniprot ID:
P16152
Entry Name:
CBR1_HUMAN
Status:
reviewed
Protein Names:
Carbonyl reductase [NADPH] 1 (EC 1.1.1.184) (15-hydroxyprostaglandin dehydrogenase [NADP(+)]) (EC 1.1.1.197) (NADPH-dependent carbonyl reductase 1) (Prostaglandin 9-ketoreductase) (Prostaglandin-E(2) 9-reductase) (EC 1.1.1.189) (Short chain dehydrogenase/reductase family 21C member 1)
Gene Names:
CBR1 CBR CRN SDR21C1
Gene Names Primary:
CBR1
Organism:
Homo sapiens (Human)

Structure

Length:
277
Sequence:
MSSGIHVALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQGRVVNVSSIMSVRALKSCSPELQQKFRSETITEEELVGLMNKFVEDTKKGVHQKEGWPSSAYGVTKIGVTVLSRIHARKLSEQRKGDKILLNACCPGWVRTDMAGPKATKSPEEGAETPVYLALLPPDAEGPHGQFVSEKRVEQW
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm.

Function

Function:
NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
Catalytic Activity:
R-CHOH-R' + NADP(+) = R-CO-R' + NADPH.; (5Z,13E)-(15S)-9-alpha,11-alpha,15-trihydroxyprosta-5,13-dienoate + NADP(+) = (5Z,13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH.; (13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprost-13-enoate + NADP(+) = (13E)-11-alpha-hydroxy-9,15-dioxoprost-13-enoate + NADPH.
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=30 uM for S-nitrosoglutathione
Enzyme Regulation:
ENZYME REGULATION: Inhibited by quercetin, rutenin and its derivatives.
Active Site:
ACT_SITE 194 194 Proton acceptor.
Cross Reference Drug Bank:
DB00997 DB00502 DB01046 DB04844
Gene Ontology Go:
cytosol
extracellular exosome
extracellular vesicle
15-hydroxyprostaglandin dehydrogenase (NADP+) activity
carbonyl reductase (NADPH) activity
oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
prostaglandin-E2 9-reductase activity
arachidonic acid metabolic process
cyclooxygenase pathway
drug metabolic process
epithelial cell differentiation
oxidation-reduction process
small molecule metabolic process
vitamin K metabolic process
Gene Ontology Biological Process:
arachidonic acid metabolic process
cyclooxygenase pathway
drug metabolic process
epithelial cell differentiation
oxidation-reduction process
small molecule metabolic process
vitamin K metabolic process
Gene Ontology Molecular Function:
15-hydroxyprostaglandin dehydrogenase (NADP+) activity
carbonyl reductase (NADPH) activity
oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
prostaglandin-E2 9-reductase activity
Gene Ontology Cellular Component:
cytosol
extracellular exosome
extracellular vesicle
Keywords:
3D-structure
Acetylation
Alternative splicing
Complete proteome
Cytoplasm
Direct protein sequencing
NADP
Oxidoreductase
Phosphoprotein
Polymorphism
Reference proteome

Publication

PubMed ID:
3141401 2182121 1921984 9740676 14702039 10830953 15489334 8421682 18449627 19413330 21269460 15799708 17912391 18826943 17344335