Names & Taxonomy
- Uniprot ID:
- P16152
- Entry Name:
- CBR1_HUMAN
- Status:
- reviewed
- Protein Names:
- Carbonyl reductase [NADPH] 1 (EC 1.1.1.184) (15-hydroxyprostaglandin dehydrogenase [NADP(+)]) (EC 1.1.1.197) (NADPH-dependent carbonyl reductase 1) (Prostaglandin 9-ketoreductase) (Prostaglandin-E(2) 9-reductase) (EC 1.1.1.189) (Short chain dehydrogenase/reductase family 21C member 1)
- Gene Names:
- CBR1 CBR CRN SDR21C1
- Gene Names Primary:
- CBR1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 277
- Sequence:
- MSSGIHVALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQGRVVNVSSIMSVRALKSCSPELQQKFRSETITEEELVGLMNKFVEDTKKGVHQKEGWPSSAYGVTKIGVTVLSRIHARKLSEQRKGDKILLNACCPGWVRTDMAGPKATKSPEEGAETPVYLALLPPDAEGPHGQFVSEKRVEQW
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm.
Function
- Function:
- NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
- Catalytic Activity:
- R-CHOH-R' + NADP(+) = R-CO-R' + NADPH.; (5Z,13E)-(15S)-9-alpha,11-alpha,15-trihydroxyprosta-5,13-dienoate + NADP(+) = (5Z,13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH.; (13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprost-13-enoate + NADP(+) = (13E)-11-alpha-hydroxy-9,15-dioxoprost-13-enoate + NADPH.
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=30 uM for S-nitrosoglutathione
- Enzyme Regulation:
- ENZYME REGULATION: Inhibited by quercetin, rutenin and its derivatives.
- Active Site:
- ACT_SITE 194 194 Proton acceptor.
- Cross Reference Drug Bank:
- DB00997 DB00502 DB01046 DB04844
- Gene Ontology Go:
- cytosol
extracellular exosome
extracellular vesicle
15-hydroxyprostaglandin dehydrogenase (NADP+) activity
carbonyl reductase (NADPH) activity
oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
prostaglandin-E2 9-reductase activity
arachidonic acid metabolic process
cyclooxygenase pathway
drug metabolic process
epithelial cell differentiation
oxidation-reduction process
small molecule metabolic process
vitamin K metabolic process - Gene Ontology Biological Process:
- arachidonic acid metabolic process
cyclooxygenase pathway
drug metabolic process
epithelial cell differentiation
oxidation-reduction process
small molecule metabolic process
vitamin K metabolic process - Gene Ontology Molecular Function:
- 15-hydroxyprostaglandin dehydrogenase (NADP+) activity
carbonyl reductase (NADPH) activity
oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
prostaglandin-E2 9-reductase activity - Gene Ontology Cellular Component:
- cytosol
extracellular exosome
extracellular vesicle - Keywords:
- 3D-structure
Acetylation
Alternative splicing
Complete proteome
Cytoplasm
Direct protein sequencing
NADP
Oxidoreductase
Phosphoprotein
Polymorphism
Reference proteome