Names & Taxonomy
- Uniprot ID:
- P11509
- Entry Name:
- CP2A6_HUMAN
- Status:
- reviewed
- Protein Names:
- Cytochrome P450 2A6 (EC 1.14.13.-) (1,4-cineole 2-exo-monooxygenase) (CYPIIA6) (Coumarin 7-hydroxylase) (Cytochrome P450 IIA3) (Cytochrome P450(I))
- Gene Names:
- CYP2A6 CYP2A3
- Gene Names Primary:
- CYP2A6
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 494
- Sequence:
- MLASGMLLVALLVCLTVMVLMSVWQQRKSKGKLPPGPTPLPFIGNYLQLNTEQMYNSLMKISERYGPVFTIHLGPRRVVVLCGHDAVREALVDQAEEFSGRGEQATFDWVFKGYGVVFSNGERAKQLRRFSIATLRDFGVGKRGIEERIQEEAGFLIDALRGTGGANIDPTFFLSRTVSNVISSIVFGDRFDYKDKEFLSLLRMMLGIFQFTSTSTGQLYEMFSSVMKHLPGPQQQAFQLLQGLEDFIAKKVEHNQRTLDPNSPRDFIDSFLIRMQEEEKNPNTEFYLKNLVMTTLNLFIGGTETVSTTLRYGFLLLMKHPEVEAKVHEEIDRVIGKNRQPKFEDRAKMPYMEAVIHEIQRFGDVIPMSLARRVKKDTKFRDFFLPKGTEVFPMLGSVLRDPSFFSNPQDFNPQHFLNEKGQFKKSDAFVPFSIGKRNCFGEGLARMELFLFFTTVMQNFRLKSSQSPKDIDVSPKHVGFATIPRNYTMSFLPR
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Function
- Function:
- Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Acts as a 1,4-cineole 2-exo-monooxygenase. Possesses low phenacetin O-deethylation activity.
- Catalytic Activity:
- 1,4-cineole + NADPH + O(2) = 2-exo-hydroxy-1,4-cineole + NADP(+) + H(2)O.
- Cofactor:
- COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.23 uM for coumarin
- Cross Reference Drug Bank:
- DB00316 DB01118 DB00381 DB01351 DB00182 DB01435 DB01274 DB00972 DB00207 DB00921 DB01156 DB00356 DB00568 DB00604 DB00636 DB00882 DB00257 DB00363 DB00531 DB06292 DB01151 DB01234 DB01191 DB00255 DB00470 DB00216 DB04953 DB04841 DB01544 DB00544 DB00690 DB01213 DB00983 DB01159 DB01181 DB00951 DB01026 DB01006 DB00281 DB04871 DB01043 DB00170 DB00763 DB00553 DB01028 DB01011 DB01110 DB00471 DB00238 DB00184 DB01115 DB06712 DB01059 DB00312 DB01174 DB01085 DB00860 DB00396 DB00818 DB01045 DB00412 DB01037 DB01236 DB06729 DB00675 DB00752 DB00755 DB01361 DB00313 DB09068 DB00495
- Gene Ontology Go:
- cytoplasmic microtubule
endoplasmic reticulum membrane
arachidonic acid epoxygenase activity
coumarin 7-hydroxylase activity
enzyme binding
heme binding
iron ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
steroid hydroxylase activity
coumarin catabolic process
coumarin metabolic process
drug metabolic process
epoxygenase P450 pathway
exogenous drug catabolic process
small molecule metabolic process
steroid metabolic process
xenobiotic metabolic process - Gene Ontology Biological Process:
- coumarin catabolic process
coumarin metabolic process
drug metabolic process
epoxygenase P450 pathway
exogenous drug catabolic process
small molecule metabolic process
steroid metabolic process
xenobiotic metabolic process - Gene Ontology Molecular Function:
- arachidonic acid epoxygenase activity
coumarin 7-hydroxylase activity
enzyme binding
heme binding
iron ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
steroid hydroxylase activity - Gene Ontology Cellular Component:
- cytoplasmic microtubule
endoplasmic reticulum membrane - Keywords:
- 3D-structure
Complete proteome
Direct protein sequencing
Endoplasmic reticulum
Heme
Iron
Membrane
Metal-binding
Microsome
Monooxygenase
NADP
Oxidoreductase
Phosphoprotein
Polymorphism
Reference proteome