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Names & Taxonomy

Uniprot ID:: P11388
Entry Name:: TOP2A_HUMAN
Status:: reviewed
Protein Names:: DNA topoisomerase 2-alpha (EC 5.99.1.3) (DNA topoisomerase II, alpha isozyme)
Gene Names:: TOP2A TOP2
Gene Names Primary:: TOP2A
Organism:: Homo sapiens (Human)

Structure

Length:: 1531
Sequence:: MEVSPLQPVNENMQVNKIKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF
Proteomes:: UP000005640

Subcellular location

Subcellular Location:: Cytoplasm

Function

Function:: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Catalytic Activity:: ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Cofactor:: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Enzyme Regulation:: ENZYME REGULATION: Specifically inhibited by the intercalating agent amsacrine.
Active Site:: ACT_SITE 805 805 O-(5'-phospho-DNA)-tyrosine intermediate.
Cross Reference Drug Bank:: DB00276 DB00537 DB00970 DB00694 DB00380 DB00997 DB00467 DB00445 DB00773 DB09047 DB04576 DB01177 DB01137 DB00978 DB04967 DB01204 DB00218 DB01059 DB01165 DB00487 DB01179 DB01208 DB00444 DB00685 DB00385
Gene Ontology Go:: condensed chromosome
cytoplasm
DNA topoisomerase complex (ATP-hydrolyzing)
nucleolus
nucleoplasm
nucleus
protein complex
ATP binding
chromatin binding
DNA binding
DNA binding, bending
DNA topoisomerase type II (ATP-hydrolyzing) activity
DNA-dependent ATPase activity
drug binding
enzyme binding
histone deacetylase binding
magnesium ion binding
poly(A) RNA binding
protein C-terminus binding
protein heterodimerization activity
protein homodimerization activity
protein kinase C binding
ubiquitin binding
apoptotic chromosome condensation
cellular protein metabolic process
cellular response to DNA damage stimulus
chromosome segregation
DNA ligation
DNA topological change
embryonic cleavage
hematopoietic progenitor cell differentiation
mitotic cell cycle
mitotic DNA integrity checkpoint
positive regulation of apoptotic process
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral genome replication
post-translational protein modification
protein sumoylation
regulation of circadian rhythm
resolution of meiotic recombination intermediates
rhythmic process
sister chromatid segregation
Gene Ontology Biological Process:: apoptotic chromosome condensation
cellular protein metabolic process
cellular response to DNA damage stimulus
chromosome segregation
DNA ligation
DNA topological change
embryonic cleavage
hematopoietic progenitor cell differentiation
mitotic cell cycle
mitotic DNA integrity checkpoint
positive regulation of apoptotic process
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral genome replication
post-translational protein modification
protein sumoylation
regulation of circadian rhythm
resolution of meiotic recombination intermediates
rhythmic process
sister chromatid segregation
Gene Ontology Molecular Function:: ATP binding
chromatin binding
DNA binding
DNA binding, bending
DNA-dependent ATPase activity
DNA topoisomerase type II (ATP-hydrolyzing) activity
drug binding
enzyme binding
histone deacetylase binding
magnesium ion binding
poly(A) RNA binding
protein C-terminus binding
protein heterodimerization activity
protein homodimerization activity
protein kinase C binding
ubiquitin binding
Gene Ontology Cellular Component:: condensed chromosome
cytoplasm
DNA topoisomerase complex (ATP-hydrolyzing)
nucleolus
nucleoplasm
nucleus
protein complex
Keywords:: 3D-structure
ATP-binding
Acetylation
Alternative splicing
Biological rhythms
Complete proteome
Cytoplasm
DNA-binding
Direct protein sequencing
Isomerase
Isopeptide bond
Magnesium
Metal-binding
Nucleotide-binding
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
Topoisomerase
Ubl conjugation
Interacts With:: P35222; Q05655

Names & Taxonomy

Structure

Subcellular location

Function

Publication