Names & Taxonomy
- Uniprot ID:
- P08684
- Entry Name:
- CP3A4_HUMAN
- Status:
- reviewed
- Protein Names:
- Cytochrome P450 3A4 (EC 1.14.13.-) (1,8-cineole 2-exo-monooxygenase) (EC 1.14.13.157) (Albendazole monooxygenase) (EC 1.14.13.32) (Albendazole sulfoxidase) (CYPIIIA3) (CYPIIIA4) (Cholesterol 25-hydroxylase) (EC 1.14.14.1) (Cytochrome P450 3A3) (Cytochrome P450 HLp) (Cytochrome P450 NF-25) (Cytochrome P450-PCN1) (Nifedipine oxidase) (Quinine 3-monooxygenase) (EC 1.14.13.67) (Taurochenodeoxycholate 6-alpha-hydroxylase) (EC 1.14.13.97)
- Gene Names:
- CYP3A4 CYP3A3
- Gene Names Primary:
- CYP3A4
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 503
- Sequence:
- MALIPDLAMETWLLLAVSLVLLYLYGTHSHGLFKKLGIPGPTPLPFLGNILSYHKGFCMFDMECHKKYGKVWGFYDGQQPVLAITDPDMIKTVLVKECYSVFTNRRPFGPVGFMKSAISIAEDEEWKRLRSLLSPTFTSGKLKEMVPIIAQYGDVLVRNLRREAETGKPVTLKDVFGAYSMDVITSTSFGVNIDSLNNPQDPFVENTKKLLRFDFLDPFFLSITVFPFLIPILEVLNICVFPREVTNFLRKSVKRMKESRLEDTQKHRVDFLQLMIDSQNSKETESHKALSDLELVAQSIIFIFAGYETTSSVLSFIMYELATHPDVQQKLQEEIDAVLPNKAPPTYDTVLQMEYLDMVVNETLRLFPIAMRLERVCKKDVEINGMFIPKGVVVMIPSYALHRDPKYWTEPEKFLPERFSKKNKDNIDPYIYTPFGSGPRNCIGMRFALMNMKLALIRVLQNFSFKPCKETQIPLKLSLGGLLQPEKPVVLKVESRDGTVSGA
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane
Function
- Function:
- Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide (PubMed:11159812). Catalyzes 4-beta-hydroxylation of cholesterol. May catalyze 25-hydroxylation of cholesterol in vitro (PubMed:21576599).
- Catalytic Activity:
- Quinine + NADPH + O(2) = 3-hydroxyquinine + NADP(+) + H(2)O.
- Cofactor:
- COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413;
- Cross Reference Drug Bank:
- DB05812 DB01418 DB00316 DB00819 DB00546 DB05773 DB00518 DB00240 DB00041 DB00802 DB00346 DB01246 DB09026 DB01431 DB00918 DB06203 DB00969 DB00404 DB06403 DB06742 DB00357 DB01424 DB01223 DB01118 DB00321 DB00381 DB00701 DB01217 DB01435 DB06605 DB05676 DB00673 DB00278 DB01238 DB06413 DB01169 DB06697 DB06216 DB00637 DB01072 DB00289 DB01076 DB06237 DB06626 DB00719 DB00972 DB00207 DB08903 DB00865 DB06770 DB00443 DB00307 DB01393 DB01128 DB04794 DB00612 DB08873 DB00188 DB00559 DB06616 DB08870 DB09128 DB01194 DB01558 DB01200 DB00835 DB01222 DB00297 DB00921 DB01156 DB00490 DB01008 DB06772 DB00248 DB08875 DB00201 DB00136 DB08907 DB06774 DB00564 DB00748 DB06016 DB01136 DB00520 DB01333 DB00482 DB04846 DB00567 DB00185 DB06777 DB00446 DB00475 DB00608 DB01114 DB00856 DB00477 DB00356 DB00169 DB01410 DB01166 DB00501 DB01012 DB00537 DB00604 DB00215 DB01211 DB00283 DB04920 DB01190 DB00349 DB00845 DB00636 DB00882 DB01242 DB01068 DB00575 DB00758 DB00628 DB01559 DB00257 DB00363 DB05239 DB00907 DB00318 DB01394 DB00872 DB00286 DB01380 DB08865 DB00924 DB00531 DB00091 DB00987 DB08912 DB09102 DB01764 DB01406 DB01219 DB06292 DB00250 DB00496 DB01264 DB01254 DB00694 DB01609 DB00705 DB01151 DB06700 DB01234 DB00514 DB00647 DB00829 DB00586 DB00485 DB00255 DB01396 DB00390 DB01551 DB00320 DB00343 DB01093 DB00280 DB00822 DB01248 DB00204 DB00757 DB08930 DB01184 DB00843 DB00869 DB01142 DB00997 DB00254 DB00470 DB04855 DB01126 DB01127 DB00625 DB00216 DB09039 DB09101 DB00584 DB08899 DB00751 DB00668 DB00700 DB00153 DB01049 DB01253 DB00696 DB00530 DB00199 DB01175 DB09119 DB00736 DB01215 DB00783 DB08866 DB01196 DB00655 DB04574 DB00402 DB00898 DB00977 DB00593 DB01466 DB00294 DB00773 DB01628 DB06414 DB01590 DB00990 DB00973 DB00426 DB00949 DB01023 DB01039 DB00813 DB06702 DB01216 DB08868 DB04908 DB00301 DB00196 DB00180 DB01544 DB00324 DB00472 DB00690 DB00499 DB08906 DB00588 DB01095 DB00176 DB01319 DB01320 DB00947 DB00674 DB00317 DB01241 DB01067 DB01016 DB00889 DB00400 DB01170 DB01018 DB01218 DB00502 DB01159 DB01355 DB00667 DB01275 DB00956 DB00769 DB00741 DB00327 DB06789 DB09053 DB09054 DB01181 DB04946 DB00619 DB00458 DB00724 DB05039 DB00808 DB00224 DB00332 DB01029 DB00762 DB06636 DB00951 DB00883 DB01020 DB00270 DB01167 DB09083 DB08820 DB00602 DB04845 DB09570 DB01321 DB01221 DB01587 DB06738 DB01026 DB00448 DB01259 DB09078 DB00528 DB01006 DB01002 DB06282 DB01137 DB01227 DB08918 DB00367 DB00451 DB00281 DB08882 DB00589 DB08827 DB01206 DB00836 DB01601 DB00455 DB04871 DB00678 DB00227 DB08933 DB06708 DB08815 DB08932 DB04835 DB00643 DB00603 DB00358 DB00814 DB01071 DB00333 DB00703 DB00763 DB00553 DB01028 DB00353 DB00959 DB06710 DB00916 DB01011 DB00379 DB06148 DB01110 DB00683 DB00834 DB08893 DB00370 DB01204 DB00745 DB00471 DB00295 DB00688 DB00607 DB09049 DB01183 DB00731 DB01149 DB00220 DB00238 DB00622 DB02701 DB00184 DB01115 DB04868 DB06712 DB00393 DB00401 DB01595 DB01054 DB00435 DB00717 DB01059 DB00540 DB00334 DB09074 DB00768 DB00338 DB00904 DB01083 DB01173 DB09330 DB04938 DB00842 DB00776 DB00239 DB01062 DB00497 DB01192 DB01229 DB09073 DB01267 DB00377 DB06603 DB00213 DB00617 DB01384 DB08439 DB00910 DB06589 DB00082 DB00738 DB00312 DB08883 DB01186 DB01074 DB04930 DB00850 DB00454 DB00780 DB01174 DB00925 DB00946 DB00812 DB00252 DB01085 DB00337 DB01100 DB01132 DB01621 DB01179 DB08910 DB08901 DB01263 DB01411 DB06209 DB00175 DB01588 DB01058 DB00860 DB00635 DB01087 DB00794 DB01032 DB00433 DB00396 DB01131 DB00420 DB01182 DB00818 DB00571 DB00339 DB00545 DB01589 DB01224 DB01103 DB00908 DB00468 DB01369 DB01129 DB00481 DB00980 DB00863 DB00243 DB00234 DB08896 DB00912 DB01256 DB00615 DB01045 DB01201 DB01220 DB08864 DB06155 DB00734 DB00503 DB06228 DB00989 DB09291 DB01301 DB00268 DB00296 DB01098 DB05271 DB00778 DB06201 DB08877 DB01001 DB00938 DB01232 DB06335 DB01037 DB11362 DB06144 DB01104 DB01236 DB01105 DB00203 DB06207 DB06290 DB00641 DB00877 DB01261 DB06268 DB01591 DB09143 DB00398 DB00708 DB00359 DB01015 DB00259 DB01138 DB01268 DB00864 DB00820 DB00675 DB00706 DB01349 DB05521 DB00976 DB00231 DB00853 DB06287 DB00444 DB00857 DB00624 DB00759 DB00277 DB00599 DB04572 DB00906 DB08816 DB00208 DB00911 DB01007 DB01409 DB00932 DB08895 DB08811 DB01036 DB06212 DB00273 DB01030 DB00539 DB05109 DB00193 DB08911 DB00752 DB00656 DB00755 DB00620 DB00897 DB00347 DB00440 DB00726 DB01361 DB06267 DB08867 DB00313 DB05294 DB00862 DB08881 DB00285 DB00661 DB09082 DB06684 DB00570 DB00541 DB00309 DB00361 DB08828 DB00582 DB09068 DB00682 DB01392 DB00549 DB00943 DB00962 DB00495 DB00744 DB00246 DB00425 DB00909 DB01198
- Gene Ontology Go:
- cytoplasm
endoplasmic reticulum membrane
integral component of membrane
intracellular membrane-bounded organelle
albendazole monooxygenase activity
aromatase activity
caffeine oxidase activity
enzyme binding
heme binding
iron ion binding
monooxygenase activity
oxidoreductase activity
oxygen binding
quinine 3-monooxygenase activity
steroid binding
steroid hydroxylase activity
taurochenodeoxycholate 6alpha-hydroxylase activity
testosterone 6-beta-hydroxylase activity
vitamin D 24-hydroxylase activity
vitamin D3 25-hydroxylase activity
alkaloid catabolic process
androgen metabolic process
calcitriol biosynthetic process from calciol
drug catabolic process
drug metabolic process
exogenous drug catabolic process
heterocycle metabolic process
lipid metabolic process
monoterpenoid metabolic process
oxidation-reduction process
oxidative demethylation
small molecule metabolic process
steroid catabolic process
steroid metabolic process
vitamin D metabolic process
xenobiotic metabolic process - Gene Ontology Biological Process:
- alkaloid catabolic process
androgen metabolic process
calcitriol biosynthetic process from calciol
drug catabolic process
drug metabolic process
exogenous drug catabolic process
heterocycle metabolic process
lipid metabolic process
monoterpenoid metabolic process
oxidation-reduction process
oxidative demethylation
small molecule metabolic process
steroid catabolic process
steroid metabolic process
vitamin D metabolic process
xenobiotic metabolic process - Gene Ontology Molecular Function:
- albendazole monooxygenase activity
aromatase activity
caffeine oxidase activity
enzyme binding
heme binding
iron ion binding
monooxygenase activity
oxidoreductase activity
oxygen binding
quinine 3-monooxygenase activity
steroid binding
steroid hydroxylase activity
taurochenodeoxycholate 6alpha-hydroxylase activity
testosterone 6-beta-hydroxylase activity
vitamin D 24-hydroxylase activity
vitamin D3 25-hydroxylase activity - Gene Ontology Cellular Component:
- cytoplasm
endoplasmic reticulum membrane
integral component of membrane
intracellular membrane-bounded organelle - Keywords:
- 3D-structure
Complete proteome
Direct protein sequencing
Endoplasmic reticulum
Heme
Iron
Lipid metabolism
Membrane
Metal-binding
Microsome
Monooxygenase
NADP
Oxidoreductase
Polymorphism
Reference proteome
Steroid metabolism
Sterol metabolism
Transmembrane
Transmembrane helix
Ubl conjugation