Names & Taxonomy
- Uniprot ID:
- O75116
- Entry Name:
- ROCK2_HUMAN
- Status:
- reviewed
- Protein Names:
- Rho-associated protein kinase 2 (EC 2.7.11.1) (Rho kinase 2) (Rho-associated, coiled-coil-containing protein kinase 2) (Rho-associated, coiled-coil-containing protein kinase II) (ROCK-II) (p164 ROCK-2)
- Gene Names:
- ROCK2 KIAA0619
- Gene Names Primary:
- ROCK2
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 1388
- Sequence:
- MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm. Cell membrane
Function
- Function:
- Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
- Catalytic Activity:
- ATP + a protein = ADP + a phosphoprotein.
- Cofactor:
- COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Enzyme Regulation:
- ENZYME REGULATION: Activated by RHOA binding. Inhibited by Y-27632 (By similarity).
- Active Site:
- ACT_SITE 214 214 Proton acceptor.
- Gene Ontology Go:
- centrosome
cytoplasmic ribonucleoprotein granule
cytosol
nucleus
plasma membrane
ATP binding
metal ion binding
poly(A) RNA binding
protein serine/threonine kinase activity
Rho-dependent protein serine/threonine kinase activity
structural molecule activity
axon guidance
cellular response to testosterone stimulus
centrosome duplication
cortical actin cytoskeleton organization
cytokinesis
ephrin receptor signaling pathway
establishment of protein localization to plasma membrane
I-kappaB kinase/NF-kappaB signaling
negative regulation of angiogenesis
negative regulation of bicellular tight junction assembly
negative regulation of myosin-light-chain-phosphatase activity
positive regulation of centrosome duplication
positive regulation of endothelial cell migration
positive regulation of gene expression
positive regulation of protein phosphorylation
protein phosphorylation
regulation of actin cytoskeleton organization
regulation of cell adhesion
regulation of cell motility
regulation of circadian rhythm
regulation of establishment of cell polarity
regulation of establishment of endothelial barrier
regulation of focal adhesion assembly
regulation of keratinocyte differentiation
regulation of stress fiber assembly
Rho protein signal transduction
rhythmic process
small GTPase mediated signal transduction
smooth muscle contraction
vascular endothelial growth factor receptor signaling pathway - Gene Ontology Biological Process:
- axon guidance
cellular response to testosterone stimulus
centrosome duplication
cortical actin cytoskeleton organization
cytokinesis
ephrin receptor signaling pathway
establishment of protein localization to plasma membrane
I-kappaB kinase/NF-kappaB signaling
negative regulation of angiogenesis
negative regulation of bicellular tight junction assembly
negative regulation of myosin-light-chain-phosphatase activity
positive regulation of centrosome duplication
positive regulation of endothelial cell migration
positive regulation of gene expression
positive regulation of protein phosphorylation
protein phosphorylation
regulation of actin cytoskeleton organization
regulation of cell adhesion
regulation of cell motility
regulation of circadian rhythm
regulation of establishment of cell polarity
regulation of establishment of endothelial barrier
regulation of focal adhesion assembly
regulation of keratinocyte differentiation
regulation of stress fiber assembly
Rho protein signal transduction
rhythmic process
small GTPase mediated signal transduction
smooth muscle contraction
vascular endothelial growth factor receptor signaling pathway - Gene Ontology Molecular Function:
- ATP binding
metal ion binding
poly(A) RNA binding
protein serine/threonine kinase activity
Rho-dependent protein serine/threonine kinase activity
structural molecule activity - Gene Ontology Cellular Component:
- centrosome
cytoplasmic ribonucleoprotein granule
cytosol
nucleus
plasma membrane - Keywords:
- 3D-structure
ATP-binding
Biological rhythms
Cell membrane
Coiled coil
Complete proteome
Cytoplasm
Cytoskeleton
Kinase
Magnesium
Membrane
Metal-binding
Nucleotide-binding
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
Serine/threonine-protein kinase
Transferase
Zinc
Zinc-finger