Names & Taxonomy

Uniprot ID:
O14727
Entry Name:
APAF_HUMAN
Status:
reviewed
Protein Names:
Apoptotic protease-activating factor 1 (APAF-1)
Gene Names:
APAF1 KIAA0413
Gene Names Primary:
APAF1
Organism:
Homo sapiens (Human)

Structure

Length:
1248
Sequence:
MDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDGIPVVSSSSGKDSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTEKNCSQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEWINKKNITNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDEHSEQVNCCHFTNSSHHLLLATGSSDCFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQEDMEVIVKCCSWSADGARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYCDFSPQNHLAVVALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHIRRLQLINGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDENGAIEILELVNNRIFQSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWNWQLDKCIFLRGHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTVLSCDISHDATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGDDNGEIRIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAGGYIKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQTLE
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm

Function

Function:
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Cross Reference Drug Bank:
DB00171
Gene Ontology Go:
apoptosome
cytosol
extracellular exosome
nucleus
ADP binding
ATP binding
cysteine-type endopeptidase activator activity involved in apoptotic process
nucleotide binding
activation of cysteine-type endopeptidase activity involved in apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
aging
apoptotic process
cardiac muscle cell apoptotic process
cell differentiation
cellular response to transforming growth factor beta stimulus
forebrain development
glial cell apoptotic process
intrinsic apoptotic signaling pathway
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
nervous system development
neural tube closure
neuron apoptotic process
positive regulation of apoptotic process
positive regulation of apoptotic signaling pathway
programmed cell death
protein homooligomerization
regulation of apoptotic DNA fragmentation
regulation of apoptotic process
response to G1 DNA damage checkpoint signaling
response to hypoxia
response to nutrient
Gene Ontology Biological Process:
activation of cysteine-type endopeptidase activity involved in apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
aging
apoptotic process
cardiac muscle cell apoptotic process
cell differentiation
cellular response to transforming growth factor beta stimulus
forebrain development
glial cell apoptotic process
intrinsic apoptotic signaling pathway
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
nervous system development
neural tube closure
neuron apoptotic process
positive regulation of apoptotic process
positive regulation of apoptotic signaling pathway
programmed cell death
protein homooligomerization
regulation of apoptotic DNA fragmentation
regulation of apoptotic process
response to G1 DNA damage checkpoint signaling
response to hypoxia
response to nutrient
Gene Ontology Molecular Function:
ADP binding
ATP binding
cysteine-type endopeptidase activator activity involved in apoptotic process
nucleotide binding
Gene Ontology Cellular Component:
apoptosome
cytosol
extracellular exosome
nucleus
Keywords:
3D-structure
ATP-binding
Alternative splicing
Apoptosis
Calcium
Complete proteome
Cytoplasm
Direct protein sequencing
Nucleotide-binding
Reference proteome
Repeat
WD repeat
Interacts With:
P55211; P99999; P62136; P62258

Publication

PubMed ID:
9267021 10441496 10364241 10393175 12804598 9455477 12168954 15489334 9651578 11389439 15262985 17244527 21371431 10543941 10578182