Names & Taxonomy
- Uniprot ID:
- Q9Y6D6
- Entry Name:
- BIG1_HUMAN
- Status:
- reviewed
- Protein Names:
- Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (Brefeldin A-inhibited GEP 1) (ADP-ribosylation factor guanine nucleotide-exchange factor 1) (p200 ARF guanine nucleotide exchange factor) (p200 ARF-GEP1)
- Gene Names:
- ARFGEF1 ARFGEP1 BIG1
- Gene Names Primary:
- ARFGEF1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 1849
- Sequence:
- MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEMSEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPNSGETAPPPPSPVSEKPLDTISQKSVDIHDSIQPRSVDNRPQAPLVSASAVNEEVSKIKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Golgi apparatus, trans-Golgi network
Function
- Function:
- Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined.
- Enzyme Regulation:
- ENZYME REGULATION: Inhibited by brefeldin A.
- Gene Ontology Go:
- cytoplasm
cytosol
Golgi apparatus
Golgi membrane
nuclear matrix
nucleolus
nucleoplasm
perinuclear region of cytoplasm
small nuclear ribonucleoprotein complex
trans-Golgi network
ARF guanyl-nucleotide exchange factor activity
guanyl-nucleotide exchange factor activity
myosin binding
protein kinase A regulatory subunit binding
endomembrane system organization
exocytosis
Golgi organization
negative regulation of actin filament polymerization
negative regulation of GTPase activity
neuron projection development
positive regulation of GTPase activity
positive regulation of protein glycosylation in Golgi
positive regulation of protein kinase B signaling
positive regulation of wound healing
protein transport
regulation of ARF protein signal transduction
regulation of establishment of cell polarity - Gene Ontology Biological Process:
- endomembrane system organization
exocytosis
Golgi organization
negative regulation of actin filament polymerization
negative regulation of GTPase activity
neuron projection development
positive regulation of GTPase activity
positive regulation of protein glycosylation in Golgi
positive regulation of protein kinase B signaling
positive regulation of wound healing
protein transport
regulation of ARF protein signal transduction
regulation of establishment of cell polarity - Gene Ontology Molecular Function:
- ARF guanyl-nucleotide exchange factor activity
guanyl-nucleotide exchange factor activity
myosin binding
protein kinase A regulatory subunit binding - Gene Ontology Cellular Component:
- cytoplasm
cytosol
Golgi apparatus
Golgi membrane
nuclear matrix
nucleolus
nucleoplasm
perinuclear region of cytoplasm
small nuclear ribonucleoprotein complex
trans-Golgi network - Keywords:
- 3D-structure
Complete proteome
Cytoplasm
Golgi apparatus
Guanine-nucleotide releasing factor
Membrane
Nucleus
Phosphoprotein
Polymorphism
Protein transport
Reference proteome
Transport - Interacts With:
- Q9Y6D5; Q7Z4S6-2; Q99417; Q13459-2