Names & Taxonomy

Uniprot ID:
Q9UBK8
Entry Name:
MTRR_HUMAN
Status:
reviewed
Protein Names:
Methionine synthase reductase (MSR) (EC 1.16.1.8)
Gene Names:
MTRR
Gene Names Primary:
MTRR
Organism:
Homo sapiens (Human)

Structure

Length:
725
Sequence:
MGAASVRAGARLVEVALCSFTVTCLEVMRRFLLLYATQQGQAKAIAEEICEQAVVHGFSADLHCISESDKYDLKTETAPLVVVVSTTGTGDPPDTARKFVKEIQNQTLPVDFFAHLRYGLLGLGDSEYTYFCNGGKIIDKRLQELGARHFYDTGHADDCVGLELVVEPWIAGLWPALRKHFRSSRGQEEISGALPVASPASSRTDLVKSELLHIESQVELLRFDDSGRKDSEVLKQNAVNSNQSNVVIEDFESSLTRSVPPLSQASLNIPGLPPEYLQVHLQESLGQEESQVSVTSADPVFQVPISKAVQLTTNDAIKTTLLVELDISNTDFSYQPGDAFSVICPNSDSEVQSLLQRLQLEDKREHCVLLKIKADTKKKGATLPQHIPAGCSLQFIFTWCLEIRAIPKKAFLRALVDYTSDSAEKRRLQELCSKQGAADYSRFVRDACACLLDLLLAFPSCQPPLSLLLEHLPKLQPRPYSCASSSLFHPGKLHFVFNIVEFLSTATTEVLRKGVCTGWLALLVASVLQPNIHASHEDSGKALAPKISISPRTTNSFHLPDDPSIPIIMVGPGTGIAPFIGFLQHREKLQEQHPDGNFGAMWLFFGCRHKDRDYLFRKELRHFLKHGILTHLKVSFSRDAPVGEEEAPAKYVQDNIQLHGQQVARILLQENGHIYVCGDAKNMAKDVHDALVQIISKEVGVEKLEAMKTLATLKEEKRYLQDIWS
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Isoform B: Cytoplasm.; Isoform C: Cytoplasm.; Isoform A: Cytoplasm

Function

Function:
Involved in the reductive regeneration of cob(I)alamin (vitamin B12) cofactor required for the maintenance of methionine synthase in a functional state. Necessary for utilization of methylgroups from the folate cycle, thereby affecting transgenerational epigenetic inheritance. Folate pathway donates methyl groups necessary for cellular methylation and affects different pathways such as DNA methylation, possibly explaining the transgenerational epigenetic inheritance effects.
Catalytic Activity:
2 -methylcob(I)alamin + 2 S-adenosylhomocysteine + NADP(+) = 2 -cob(II)alamin + NADPH + 2 S-adenosyl-L-methionine.
Cofactor:
COFACTOR: Name=FAD; Xref=ChEBI:CHEBI:57692;
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.89 uM for NADPH
Cross Reference Drug Bank:
DB00115 DB00200 DB00134
Gene Ontology Go:
cytoplasm
cytosol
intermediate filament cytoskeleton
nucleoplasm
reductase activity
aquacobalamin reductase (NADPH) activity
FAD binding
flavin adenine dinucleotide binding
FMN binding
iron ion binding
NADP binding
NADPH binding
NADPH-hemoprotein reductase activity
oxidoreductase activity, oxidizing metal ions, NAD or NADP as acceptor
cellular nitrogen compound metabolic process
cobalamin metabolic process
DNA methylation
folic acid metabolic process
homocysteine catabolic process
methionine biosynthetic process
methionine metabolic process
methylation
negative regulation of cystathionine beta-synthase activity
oxidation-reduction process
S-adenosylmethionine cycle
small molecule metabolic process
sulfur amino acid metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process
xenobiotic metabolic process
Gene Ontology Biological Process:
cellular nitrogen compound metabolic process
cobalamin metabolic process
DNA methylation
folic acid metabolic process
homocysteine catabolic process
methionine biosynthetic process
methionine metabolic process
methylation
negative regulation of cystathionine beta-synthase activity
oxidation-reduction process
S-adenosylmethionine cycle
small molecule metabolic process
sulfur amino acid metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process
xenobiotic metabolic process
Gene Ontology Molecular Function:
reductase activity
aquacobalamin reductase (NADPH) activity
FAD binding
flavin adenine dinucleotide binding
FMN binding
iron ion binding
NADP binding
NADPH binding
NADPH-hemoprotein reductase activity
oxidoreductase activity, oxidizing metal ions, NAD or NADP as acceptor
Gene Ontology Cellular Component:
cytoplasm
cytosol
intermediate filament cytoskeleton
nucleoplasm
Keywords:
3D-structure
Alternative splicing
Amino-acid biosynthesis
Complete proteome
Cytoplasm
Disease mutation
FAD
FMN
Flavoprotein
Methionine biosynthesis
NADP
Oxidoreductase
Phosphoprotein
Polymorphism
Reference proteome
S-adenosyl-L-methionine
Interacts With:
Q53SE7

Publication

PubMed ID:
10564814 9501215 15372022 15489334 18221906 20068231 21269460 24275569 17892308 10484769 10444342 12375236 15979034