Names & Taxonomy
- Uniprot ID:
- Q9P2W7
- Entry Name:
- B3GA1_HUMAN
- Status:
- reviewed
- Protein Names:
- Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 (EC 2.4.1.135) (Beta-1,3-glucuronyltransferase 1) (Glucuronosyltransferase P) (GlcAT-P) (UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase) (GlcUAT-P)
- Gene Names:
- B3GAT1 GLCATP
- Gene Names Primary:
- B3GAT1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 334
- Sequence:
- MPKRRDILAIVLIVLPWTLLITVWHQSTLAPLLAVHKDEGSDPRRETPPGADPREYCTSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Golgi apparatus membrane; Single-pass type II membrane protein.
Function
- Function:
- Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group (By similarity).
- Pathway:
- Protein modification; protein glycosylation.
- Catalytic Activity:
- UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.
- Cofactor:
- COFACTOR: Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
- Active Site:
- ACT_SITE 284 284 Proton donor/acceptor.
- Gene Ontology Go:
- Golgi membrane
integral component of membrane
galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity
metal ion binding
carbohydrate metabolic process
chondroitin sulfate metabolic process
chondroitin sulfate proteoglycan biosynthetic process
glycosaminoglycan metabolic process
protein glycosylation
small molecule metabolic process - Gene Ontology Biological Process:
- carbohydrate metabolic process
chondroitin sulfate metabolic process
chondroitin sulfate proteoglycan biosynthetic process
glycosaminoglycan metabolic process
protein glycosylation
small molecule metabolic process - Gene Ontology Molecular Function:
- galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity
metal ion binding - Gene Ontology Cellular Component:
- Golgi membrane
integral component of membrane - Keywords:
- 3D-structure
Complete proteome
Glycoprotein
Golgi apparatus
Manganese
Membrane
Metal-binding
Phosphoprotein
Polymorphism
Reference proteome
Signal-anchor
Transferase
Transmembrane
Transmembrane helix