Names & Taxonomy
- Uniprot ID:
- Q96Q83
- Entry Name:
- ALKB3_HUMAN
- Status:
- reviewed
- Protein Names:
- Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 (EC 1.14.11.-) (Alkylated DNA repair protein alkB homolog 3) (hABH3) (DEPC-1) (Prostate cancer antigen 1)
- Gene Names:
- ALKBH3 ABH3 DEPC1
- Gene Names Primary:
- ALKBH3
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 286
- Sequence:
- MEEKRRRARVQGAWAAPVKSQAIAQPATTAKSHLHQKPGQTWKNKEHHLSDREFVFKEPQQVVRRAPEPRVIDREGVYEISLSPTGVSRVCLYPGFVDVKEADWILEQLCQDVPWKQRTGIREDITYQQPRLTAWYGELPYTYSRITMEPNPHWHPVLRTLKNRIEENTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEMRKKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSREPRVNLTFRTVYPDPRGAPW
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Nucleus
Function
- Function:
- Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769). Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3 (PubMed:22055184). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410, PubMed:16858410). Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:22055184, PubMed:16858410).
- Cofactor:
- COFACTOR: Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
- Enzyme Regulation:
- ENZYME REGULATION: Activated by ascorbate.
- Cross Reference Drug Bank:
- DB00126
- Gene Ontology Go:
- cytoplasm
mitochondrion
nucleoplasm
nucleus
cytosine C-5 DNA demethylase activity
DNA-N1-methyladenine dioxygenase activity
ferrous iron binding
L-ascorbic acid binding
cell proliferation
DNA dealkylation involved in DNA repair
DNA repair
oxidative single-stranded DNA demethylation - Gene Ontology Biological Process:
- cell proliferation
DNA dealkylation involved in DNA repair
DNA repair
oxidative single-stranded DNA demethylation - Gene Ontology Molecular Function:
- cytosine C-5 DNA demethylase activity
DNA-N1-methyladenine dioxygenase activity
ferrous iron binding
L-ascorbic acid binding - Gene Ontology Cellular Component:
- cytoplasm
mitochondrion
nucleoplasm
nucleus - Keywords:
- 3D-structure
Alternative splicing
Complete proteome
Cytoplasm
DNA damage
DNA repair
Dioxygenase
Hydroxylation
Iron
Metal-binding
Nucleus
Oxidation
Oxidoreductase
Polymorphism
Reference proteome
Vitamin C - Interacts With:
- Q96MA6; O76003; Q08379; Q13422; Q8TBB1