Names & Taxonomy

Uniprot ID:
Q8WUJ3
Entry Name:
CEMIP_HUMAN
Status:
reviewed
Protein Names:
Cell migration-inducing and hyaluronan-binding protein (EC 3.2.1.35)
Gene Names:
CEMIP KIAA1199
Gene Names Primary:
CEMIP
Organism:
Homo sapiens (Human)

Structure

Length:
1361
Sequence:
MGAAGRQDFLFKAMLTISWLTLTCFPGATSTVAAGCPDQSPELQPWNPGHDQDHHVHIGQGKTLLLTSSATVYSIHISEGGKLVIKDHDEPIVLRTRHILIDNGGELHAGSALCPFQGNFTIILYGRADEGIQPDPYYGLKYIGVGKGGALELHGQKKLSWTFLNKTLHPGGMAEGGYFFERSWGHRGVIVHVIDPKSGTVIHSDRFDTYRSKKESERLVQYLNAVPDGRILSVAVNDEGSRNLDDMARKAMTKLGSKHFLHLGFRHPWSFLTVKGNPSSSVEDHIEYHGHRGSAAARVFKLFQTEHGEYFNVSLSSEWVQDVEWTEWFDHDKVSQTKGGEKISDLWKAHPGKICNRPIDIQATTMDGVNLSTEVVYKKGQDYRFACYDRGRACRSYRVRFLCGKPVRPKLTVTIDTNVNSTILNLEDNVQSWKPGDTLVIASTDYSMYQAEEFQVLPCRSCAPNQVKVAGKPMYLHIGEEIDGVDMRAEVGLLSRNIIVMGEMEDKCYPYRNHICNFFDFDTFGGHIKFALGFKAAHLEGTELKHMGQQLVGQYPIHFHLAGDVDERGGYDPPTYIRDLSIHHTFSRCVTVHGSNGLLIKDVVGYNSLGHCFFTEDGPEERNTFDHCLGLLVKSGTLLPSDRDSKMCKMITEDSYPGYIPKPRQDCNAVSTFWMANPNNNLINCAAAGSEETGFWFIFHHVPTGPSVGMYSPGYSEHIPLGKFYNNRAHSNYRAGMIIDNGVKTTEASAKDKRPFLSIISARYSPHQDADPLKPREPAIIRHFIAYKNQDHGAWLRGGDVWLDSCRFADNGIGLTLASGGTFPYDDGSKQEIKNSLFVGESGNVGTEMMDNRIWGPGGLDHSGRTLPIGQNFPIRGIQLYDGPINIQNCTFRKFVALEGRHTSALAFRLNNAWQSCPHNNVTGIAFEDVPITSRVFFGEPGPWFNQLDMDGDKTSVFHDVDGSVSEYPGSYLTKNDNWLVRHPDCINVPDWRGAICSGCYAQMYIQAYKTSNLRMKIIKNDFPSHPLYLEGALTRSTHYQQYQPVVTLQKGYTIHWDQTAPAELAIWLINFNKGDWIRVGLCYPRGTTFSILSDVHNRLLKQTSKTGVFVRTLQMDKVEQSYPGRSHYYWDEDSGLLFLKLKAQNEREKFAFCSMKGCERIKIKALIPKNAGVSDCTATAYPKFTERAVVDVPMPKKLFGSQLKTKDHFLEVKMESSKQHFFHLWNDFAYIEVDGKKYPSSEDGIQVVVIDGNQGRVVSHTSFRNSILQGIPWQLFNYVATIPDNSIVLMASKGRYVSRGPWTRVLEKLGADRGLKLKEQMAFVGFKGSFRPIWVTLDTEDHKAKIFQVVPIPVVKKKKL
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Nucleus. Cytoplasm. Endoplasmic reticulum. Cell membrane. Membrane, clathrin-coated pit. Secreted. Note=Retained in the endoplasmic reticulum (ER) in a HSPA5/BIP-dependent manner. Colocalized with clathrin heavy chain/CLTC in clathrin-coated vesicles. Strongly detected in the cytoplasm of breast carcinoma cells, whereas poorly detected in adjacent normal epithelial cells, stromal cells, or benign breast tissues. Localized in the nucleus and cytoplasm of colon adenocarcinomas.

Function

Function:
Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.
Catalytic Activity:
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Enzyme Regulation:
ENZYME REGULATION: Activity is up-regulated by histamine.
Gene Ontology Go:
clathrin-coated vesicle membrane
coated pit
cytoplasm
endoplasmic reticulum
extracellular region
nucleus
plasma membrane
clathrin heavy chain binding
ER retention sequence binding
hyaluronic acid binding
hyalurononglucosaminidase activity
carbohydrate metabolic process
glycosaminoglycan metabolic process
hyaluronan biosynthetic process
hyaluronan catabolic process
hyaluronan metabolic process
positive regulation of cell migration
positive regulation of peptidyl-threonine phosphorylation
positive regulation of protein kinase C activity
positive regulation of protein targeting to membrane
positive regulation of release of sequestered calcium ion into cytosol
sensory perception of sound
small molecule metabolic process
Gene Ontology Biological Process:
carbohydrate metabolic process
glycosaminoglycan metabolic process
hyaluronan biosynthetic process
hyaluronan catabolic process
hyaluronan metabolic process
positive regulation of cell migration
positive regulation of peptidyl-threonine phosphorylation
positive regulation of protein kinase C activity
positive regulation of protein targeting to membrane
positive regulation of release of sequestered calcium ion into cytosol
sensory perception of sound
small molecule metabolic process
Gene Ontology Molecular Function:
clathrin heavy chain binding
ER retention sequence binding
hyaluronic acid binding
hyalurononglucosaminidase activity
Gene Ontology Cellular Component:
clathrin-coated vesicle membrane
coated pit
cytoplasm
endoplasmic reticulum
extracellular region
nucleus
plasma membrane
Keywords:
Alternative splicing
Cell membrane
Coated pit
Complete proteome
Cytoplasm
Endoplasmic reticulum
Glycoprotein
Glycosidase
Hyaluronic acid
Hydrolase
Membrane
Nucleus
Polymorphism
Reference proteome
Repeat
Secreted
Signal

Publication

PubMed ID:
14577002 16157444 15489334 10574462 11247670 12471561 19434458 21772334 22970280 23990668 23936024 23509262 24269685