Names & Taxonomy

Uniprot ID:
Q16678
Entry Name:
CP1B1_HUMAN
Status:
reviewed
Protein Names:
Cytochrome P450 1B1 (EC 1.14.14.1) (CYPIB1)
Gene Names:
CYP1B1
Gene Names Primary:
CYP1B1
Organism:
Homo sapiens (Human)

Structure

Length:
543
Sequence:
MGTSLSPNDPWPLNPLSIQQTTLLLLLSVLATVHVGQRLLRQRRRQLRSAPPGPFAWPLIGNAAAVGQAAHLSFARLARRYGDVFQIRLGSCPIVVLNGERAIHQALVQQGSAFADRPAFASFRVVSGGRSMAFGHYSEHWKVQRRAAHSMMRNFFTRQPRSRQVLEGHVLSEARELVALLVRGSADGAFLDPRPLTVVAVANVMSAVCFGCRYSHDDPEFRELLSHNEEFGRTVGAGSLVDVMPWLQYFPNPVRTVFREFEQLNRNFSNFILDKFLRHCESLRPGAAPRDMMDAFILSAEKKAAGDSHGGGARLDLENVPATITDIFGASQDTLSTALQWLLLLFTRYPDVQTRVQAELDQVVGRDRLPCMGDQPNLPYVLAFLYEAMRFSSFVPVTIPHATTANTSVLGYHIPKDTVVFVNQWSVNHDPLKWPNPENFDPARFLDKDGLINKDLTSRVMIFSVGKRRCIGEELSKMQLFLFISILAHQCDFRANPNEPAKMNFSYGLTIKPKSFKVNVTLRESMELLDSAVQNLQAKETCQ
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Mitochondrion

Function

Function:
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, retinoid and xenobiotics. Preferentially oxidizes 17beta-estradiol to the carcinogenic 4-hydroxy derivative, and a variety of procarcinogenic compounds to their activated forms, including polycyclic aromatic hydrocarbons. Promotes angiogenesis by removing cellular oxygenation products, thereby decreasing oxidative stress, release of antiangiogenic factor THBS2, then allowing endothelial cells migration, cell adhesion and capillary morphogenesis. These changes are concommitant with the endothelial nitric oxide synthase activity and nitric oxide synthesis. Plays an important role in the regulation of perivascular cell proliferation, migration, and survival through modulation of the intracellular oxidative state and NF-kappa-B expression and/or activity, during angiogenesis. Contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression.
Catalytic Activity:
RH + + O(2) = ROH + + H(2)O.
Cofactor:
COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413;
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: ; Kinetic parameters: KM=6.0 uM for 17-beta-estradiol
Enzyme Regulation:
ENZYME REGULATION: Enzyme activity is increased by liposomes containing anionic phospholipids, phosphatidic acid and cardiolipin. Inhibited by naringenin with an IC(50) of 5 uM.
Cross Reference Drug Bank:
DB00613 DB01169 DB00121 DB00201 DB00363 DB01254 DB00694 DB01234 DB01248 DB00997 DB00530 DB00783 DB00655 DB00499 DB01026 DB00448 DB01065 DB01204 DB00338 DB00526 DB01229 DB01174 DB01087 DB01168 DB00396 DB00818 DB00675 DB00624 DB00277
Gene Ontology Go:
endoplasmic reticulum membrane
intracellular membrane-bounded organelle
mitochondrion
aromatase activity
heme binding
iron ion binding
monooxygenase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
oxygen binding
angiogenesis
arachidonic acid metabolic process
blood vessel morphogenesis
cell adhesion
cellular aromatic compound metabolic process
cellular response to hydrogen peroxide
cellular response to organic cyclic compound
collagen fibril organization
endothelial cell migration
endothelial cell-cell adhesion
epoxygenase P450 pathway
estrogen metabolic process
intrinsic apoptotic signaling pathway in response to oxidative stress
membrane lipid catabolic process
negative regulation of cell adhesion mediated by integrin
negative regulation of cell migration
negative regulation of cell proliferation
negative regulation of NF-kappaB transcription factor activity
nitric oxide biosynthetic process
omega-hydroxylase P450 pathway
oxidation-reduction process
positive regulation of angiogenesis
positive regulation of apoptotic process
positive regulation of JAK-STAT cascade
positive regulation of vascular endothelial growth factor production
regulation of reactive oxygen species metabolic process
response to toxic substance
retinal blood vessel morphogenesis
retinal metabolic process
retinol metabolic process
small molecule metabolic process
steroid metabolic process
sterol metabolic process
toxin metabolic process
trabecular meshwork development
visual perception
xenobiotic metabolic process
Gene Ontology Biological Process:
angiogenesis
arachidonic acid metabolic process
blood vessel morphogenesis
cell adhesion
cellular aromatic compound metabolic process
cellular response to hydrogen peroxide
cellular response to organic cyclic compound
collagen fibril organization
endothelial cell-cell adhesion
endothelial cell migration
epoxygenase P450 pathway
estrogen metabolic process
intrinsic apoptotic signaling pathway in response to oxidative stress
membrane lipid catabolic process
negative regulation of cell adhesion mediated by integrin
negative regulation of cell migration
negative regulation of cell proliferation
negative regulation of NF-kappaB transcription factor activity
nitric oxide biosynthetic process
omega-hydroxylase P450 pathway
oxidation-reduction process
positive regulation of angiogenesis
positive regulation of apoptotic process
positive regulation of JAK-STAT cascade
positive regulation of vascular endothelial growth factor production
regulation of reactive oxygen species metabolic process
response to toxic substance
retinal blood vessel morphogenesis
retinal metabolic process
retinol metabolic process
small molecule metabolic process
steroid metabolic process
sterol metabolic process
toxin metabolic process
trabecular meshwork development
visual perception
xenobiotic metabolic process
Gene Ontology Molecular Function:
aromatase activity
heme binding
iron ion binding
monooxygenase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
oxygen binding
Gene Ontology Cellular Component:
endoplasmic reticulum membrane
intracellular membrane-bounded organelle
mitochondrion
Keywords:
3D-structure
Complete proteome
Disease mutation
Endoplasmic reticulum
Glaucoma
Heme
Iron
Membrane
Metal-binding
Microsome
Mitochondrion
Monooxygenase
Oxidoreductase
Peters anomaly
Polymorphism
Reference proteome

Publication

PubMed ID:
8175734 8910454 15489334 10426814 11403040 15258110 22888116 23821647 22935222 21147782 9463332 9497261 9823305 10227395 10655546 11184479 10739169 11527932 11774072 11980847 12036985 11854439 14640114 14635112 12525557 15342693 15475877 15255109 16490498 16688110 16735994 16862072 18470941