Names & Taxonomy

Uniprot ID:
Q13126
Entry Name:
MTAP_HUMAN
Status:
reviewed
Protein Names:
S-methyl-5'-thioadenosine phosphorylase (EC 2.4.2.28) (5'-methylthioadenosine phosphorylase) (MTA phosphorylase) (MTAP) (MTAPase)
Gene Names:
MTAP MSAP
Gene Names Primary:
MTAP
Organism:
Homo sapiens (Human)

Structure

Length:
283
Sequence:
MASGTTTTAVKIGIIGGTGLDDPEILEGRTEKYVDTPFGKPSDALILGKIKNVDCVLLARHGRQHTIMPSKVNYQANIWALKEEGCTHVIVTTACGSLREEIQPGDIVIIDQFIDRTTMRPQSFYDGSHSCARGVCHIPMAEPFCPKTREVLIETAKKLGLRCHSKGTMVTIEGPRFSSRAESFMFRTWGADVINMTTVPEVVLAKEAGICYASIAMATDYDCWKEHEEAVSVDRVLKTLKENANKAKSLLLTTIPQIGSTEWSETLHNLKNMAQFSVLLPRH
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm. Nucleus

Function

Function:
Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
Pathway:
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1.
Catalytic Activity:
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=5 uM for S-methyl-5'-thioadenosine
Enzyme Regulation:
ENZYME REGULATION: Inhibited by 5'-methylthiotubercin and 5'-chloroformycin.
Cross Reference Drug Bank:
DB00173
Gene Ontology Go:
cytoplasm
cytosol
extracellular exosome
nucleus
phosphorylase activity
S-methyl-5-thioadenosine phosphorylase activity
cellular nitrogen compound metabolic process
L-methionine biosynthetic process from methylthioadenosine
methylation
nucleobase-containing compound metabolic process
polyamine metabolic process
purine ribonucleoside salvage
purine-containing compound salvage
response to testosterone
small molecule metabolic process
sulfur amino acid metabolic process
Gene Ontology Biological Process:
cellular nitrogen compound metabolic process
L-methionine biosynthetic process from methylthioadenosine
methylation
nucleobase-containing compound metabolic process
polyamine metabolic process
purine-containing compound salvage
purine ribonucleoside salvage
response to testosterone
small molecule metabolic process
sulfur amino acid metabolic process
Gene Ontology Molecular Function:
phosphorylase activity
S-methyl-5-thioadenosine phosphorylase activity
Gene Ontology Cellular Component:
cytoplasm
cytosol
extracellular exosome
nucleus
Keywords:
3D-structure
Acetylation
Alternative splicing
Complete proteome
Cytoplasm
Glycosyltransferase
Nucleus
Polymorphism
Purine salvage
Reference proteome
Transferase
Interacts With:
Q9H3R5

Publication

PubMed ID:
7604019 8650244 22464254 15164053 15489334 3091600 8687427 11895909 17912432 19097084 21269460 21412930 10404592 15122881 14705926 20934997