Names & Taxonomy
- Uniprot ID:
- P78362
- Entry Name:
- SRPK2_HUMAN
- Status:
- reviewed
- Protein Names:
- SRSF protein kinase 2 (EC 2.7.11.1) (SFRS protein kinase 2) (Serine/arginine-rich protein-specific kinase 2) (SR-protein-specific kinase 2) [Cleaved into: SRSF protein kinase 2 N-terminal; SRSF protein kinase 2 C-terminal]
- Gene Names:
- SRPK2
- Gene Names Primary:
- SRPK2
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 688
- Sequence:
- MSVNSEKSSSSERPEPQQKAPLVPPPPPPPPPPPPPLPDPTPPEPEEEILGSDDEEQEDPADYCKGGYHPVKIGDLFNGRYHVIRKLGWGHFSTVWLCWDMQGKRFVAMKVVKSAQHYTETALDEIKLLKCVRESDPSDPNKDMVVQLIDDFKISGMNGIHVCMVFEVLGHHLLKWIIKSNYQGLPVRCVKSIIRQVLQGLDYLHSKCKIIHTDIKPENILMCVDDAYVRRMAAEATEWQKAGAPPPSGSAVSTAPQQKPIGKISKNKKKKLKKKQKRQAELLEKRLQEIEELEREAERKIIEENITSAAPSNDQDGEYCPEVKLKTTGLEEAAEAETAKDNGEAEDQEEKEDAEKENIEKDEDDVDQELANIDPTWIESPKTNGHIENGPFSLEQQLDDEDDDEEDCPNPEEYNLDEPNAESDYTYSSSYEQFNGELPNGRHKIPESQFPEFSTSLFSGSLEPVACGSVLSEGSPLTEQEESSPSHDRSRTVSASSTGDLPKAKTRAADLLVNPLDPRNADKIRVKIADLGNACWVHKHFTEDIQTRQYRSIEVLIGAGYSTPADIWSTACMAFELATGDYLFEPHSGEDYSRDEDHIAHIIELLGSIPRHFALSGKYSREFFNRRGELRHITKLKPWSLFDVLVEKYGWPHEDAAQFTDFLIPMLEMVPEKRASAGECLRHPWLNS
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. KAT5/TIP60 inhibits its nuclear translocation. Phosphorylation at Thr-492 by PKB/AKT1 promotes nuclear translocation.
Function
- Function:
- Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.
- Catalytic Activity:
- ATP + a protein = ADP + a phosphoprotein.
- Cofactor:
- COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Enzyme Regulation:
- ENZYME REGULATION: Activated by phosphorylation on Ser-52 and Ser-588.
- Active Site:
- ACT_SITE 214 214 Proton acceptor.
- Cross Reference Drug Bank:
- DB00173
- Gene Ontology Go:
- cytoplasm
nucleolus
nucleoplasm
nucleus
14-3-3 protein binding
ATP binding
magnesium ion binding
poly(A) RNA binding
protein serine/threonine kinase activity
angiogenesis
cell differentiation
innate immune response
intracellular signal transduction
negative regulation of viral genome replication
nuclear speck organization
positive regulation of cell cycle
positive regulation of cell proliferation
positive regulation of gene expression
positive regulation of neuron apoptotic process
positive regulation of viral genome replication
protein phosphorylation
regulation of mRNA splicing, via spliceosome
RNA splicing
spliceosomal complex assembly - Gene Ontology Biological Process:
- angiogenesis
cell differentiation
innate immune response
intracellular signal transduction
negative regulation of viral genome replication
nuclear speck organization
positive regulation of cell cycle
positive regulation of cell proliferation
positive regulation of gene expression
positive regulation of neuron apoptotic process
positive regulation of viral genome replication
protein phosphorylation
regulation of mRNA splicing, via spliceosome
RNA splicing
spliceosomal complex assembly - Gene Ontology Molecular Function:
- 14-3-3 protein binding
ATP binding
magnesium ion binding
poly(A) RNA binding
protein serine/threonine kinase activity - Gene Ontology Cellular Component:
- cytoplasm
nucleolus
nucleoplasm
nucleus - Keywords:
- 3D-structure
ATP-binding
Alternative splicing
Complete proteome
Cytoplasm
Differentiation
Kinase
Nucleotide-binding
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
Serine/threonine-protein kinase
Transferase
mRNA processing
mRNA splicing - Interacts With:
- Q08117; Q9NWB6; P49760; P49761; P51116; P07910; Q9NQ29; Q9Y383; Q9P127; Q9BU76; Q8NAV1; Q86U06; Q14498; Q9Y5S9; D3DU92; Q8WVK2; P08621; Q16629; Q9BRL6; Q01081; Q01081-2; P26368; Q96MU7; P31946; Q15696; O15535