Names & Taxonomy
- Uniprot ID:
- P53667
- Entry Name:
- LIMK1_HUMAN
- Status:
- reviewed
- Protein Names:
- LIM domain kinase 1 (LIMK-1) (EC 2.7.11.1)
- Gene Names:
- LIMK1 LIMK
- Gene Names Primary:
- LIMK1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 647
- Sequence:
- MRLTLLCCTWREERMGEEGSELPVCASCGQRIYDGQYLQALNADWHADCFRCCDCSASLSHQYYEKDGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGELKYHPECFICLTCGTFIGDGDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHVGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDTLGHGLGPETSPLSSPAYTPSGEAGSSARQKPVLRSCSIDRSPGAGSLGSPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLMVDEKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm. Nucleus. Note=Predominantly found in the cytoplasm.
Function
- Function:
- Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).
- Catalytic Activity:
- ATP + a protein = ADP + a phosphoprotein.
- Active Site:
- ACT_SITE 460 460
- Cross Reference Drug Bank:
- DB08912
- Gene Ontology Go:
- axonal growth cone
cytoplasm
cytosol
focal adhesion
Golgi apparatus
membrane
neuron projection
nucleoplasm
perinuclear region of cytoplasm
ATP binding
heat shock protein binding
protein kinase activity
protein serine/threonine kinase activity
zinc ion binding
actin cytoskeleton organization
axon guidance
ephrin receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
innate immune response
negative regulation of ubiquitin-protein transferase activity
nervous system development
positive regulation of actin filament bundle assembly
positive regulation of axon extension
positive regulation of stress fiber assembly
protein phosphorylation
Rho protein signal transduction
signal transduction
small GTPase mediated signal transduction - Gene Ontology Biological Process:
- actin cytoskeleton organization
axon guidance
ephrin receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
innate immune response
negative regulation of ubiquitin-protein transferase activity
nervous system development
positive regulation of actin filament bundle assembly
positive regulation of axon extension
positive regulation of stress fiber assembly
protein phosphorylation
Rho protein signal transduction
signal transduction
small GTPase mediated signal transduction - Gene Ontology Molecular Function:
- ATP binding
heat shock protein binding
protein kinase activity
protein serine/threonine kinase activity
zinc ion binding - Gene Ontology Cellular Component:
- axonal growth cone
cytoplasm
cytosol
focal adhesion
Golgi apparatus
membrane
neuron projection
nucleoplasm
perinuclear region of cytoplasm - Keywords:
- 3D-structure
ATP-binding
Alternative splicing
Complete proteome
Cytoplasm
Kinase
LIM domain
Metal-binding
Nucleotide-binding
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
Repeat
Serine/threonine-protein kinase
Transferase
Ubl conjugation
Williams-Beuren syndrome
Zinc - Interacts With:
- P29066; P29067; P23528; O95835; P50281; Q13153; Q13177; Q8WYL5