Names & Taxonomy

Uniprot ID:
P52848
Entry Name:
NDST1_HUMAN
Status:
reviewed
Protein Names:
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 (EC 2.8.2.8) (Glucosaminyl N-deacetylase/N-sulfotransferase 1) (NDST-1) (N-heparan sulfate sulfotransferase 1) (N-HSST 1) ([Heparan sulfate]-glucosamine N-sulfotransferase 1) (HSNST 1) [Includes: Heparan sulfate N-deacetylase 1 (EC 3.-.-.-); Heparan sulfate N-sulfotransferase 1 (EC 2.8.2.-)]
Gene Names:
NDST1 HSST HSST1
Gene Names Primary:
NDST1
Organism:
Homo sapiens (Human)

Structure

Length:
882
Sequence:
MPALACLRRLCRHVSPQAVLFLLFIFCLFSVFISAYYLYGWKRGLEPSADAPEPDCGDPPPVAPSRLLPLKPVQAATPSRTDPLVLVFVESLYSQLGQEVVAILESSRFKYRTEIAPGKGDMPTLTDKGRGRFALIIYENILKYVNLDAWNRELLDKYCVAYGVGIIGFFKANENSLLSAQLKGFPLFLHSNLGLKDCSINPKSPLLYVTRPSEVEKGVLPGEDWTVFQSNHSTYEPVLLAKTRSSESIPHLGADAGLHAALHATVVQDLGLHDGIQRVLFGNNLNFWLHKLVFVDAVAFLTGKRLSLPLDRYILVDIDDIFVGKEGTRMKVEDVKALFDTQNELRAHIPNFTFNLGYSGKFFHTGTNAEDAGDDLLLSYVKEFWWFPHMWSHMQPHLFHNQSVLAEQMALNKKFAVEHGIPTDMGYAVAPHHSGVYPVHVQLYEAWKQVWSIRVTSTEEYPHLKPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSSELDKIINGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFKHLVRFLHSWTNLRLQTLPPVQLAQKYFQIFSEEKDPLWQDPCEDKRHKDIWSKEKTCDRFPKLLIIGPQKTGTTALYLFLGMHPDLSSNYPSSETFEEIQFFNGHNYHKGIDWYMEFFPIPSNTTSDFYFEKSANYFDSEVAPRRAAALLPKAKVLTILINPADRAYSWYQHQRAHDDPVALKYTFHEVITAGSDASSKLRALQNRCLVPGWYATHIERWLSAYHANQILVLDGKLLRTEPAKVMDMVQKFLGVTNTIDYHKTLAFDPKKGFWCQLLEGGKTKCLGKSKGRKYPEMDLDSRAFLKDYYRDHNIELSKLLYKMGQTLPTWLREDLQNTR
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Golgi apparatus membrane

Function

Function:
Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis (PubMed:10758005, PubMed:12634318). Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response (PubMed:10758005, PubMed:12634318). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
Pathway:
Glycan metabolism; heparan sulfate biosynthesis.; Glycan metabolism; heparin biosynthesis.
Catalytic Activity:
3'-phosphoadenylyl sulfate + -glucosamine = adenosine 3',5'-bisphosphate + -N-sulfoglucosamine.
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=13.3 uM for K5 polysaccharide
Active Site:
ACT_SITE 614 614 For sulfotransferase activity.
Gene Ontology Go:
Golgi membrane
integral component of membrane
-glucosamine N-sulfotransferase activity
deacetylase activity
aorta development
carbohydrate metabolic process
cardiac septum development
coronary vasculature development
embryonic neurocranium morphogenesis
embryonic viscerocranium morphogenesis
fibroblast growth factor receptor signaling pathway
forebrain development
glycosaminoglycan biosynthetic process
glycosaminoglycan metabolic process
heparan sulfate proteoglycan biosynthetic process
heparin biosynthetic process
inflammatory response
MAPK cascade
midbrain development
polysaccharide biosynthetic process
protein sulfation
respiratory gaseous exchange
small molecule metabolic process
smoothened signaling pathway
Gene Ontology Biological Process:
aorta development
carbohydrate metabolic process
cardiac septum development
coronary vasculature development
embryonic neurocranium morphogenesis
embryonic viscerocranium morphogenesis
fibroblast growth factor receptor signaling pathway
forebrain development
glycosaminoglycan biosynthetic process
glycosaminoglycan metabolic process
heparan sulfate proteoglycan biosynthetic process
heparin biosynthetic process
inflammatory response
MAPK cascade
midbrain development
polysaccharide biosynthetic process
protein sulfation
respiratory gaseous exchange
small molecule metabolic process
smoothened signaling pathway
Gene Ontology Molecular Function:
-glucosamine N-sulfotransferase activity
deacetylase activity
Gene Ontology Cellular Component:
Golgi membrane
integral component of membrane
Keywords:
3D-structure
Alternative splicing
Complete proteome
Disease mutation
Disulfide bond
Glycoprotein
Golgi apparatus
Hydrolase
Inflammatory response
Membrane
Mental retardation
Multifunctional enzyme
Reference proteome
Signal-anchor
Transferase
Transmembrane
Transmembrane helix

Publication

PubMed ID:
7601448 9230113 15489334 9744796 10758005 12634318 22660413 10196134 25125150