Names & Taxonomy
- Uniprot ID:
- P52848
- Entry Name:
- NDST1_HUMAN
- Status:
- reviewed
- Protein Names:
- Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 (EC 2.8.2.8) (Glucosaminyl N-deacetylase/N-sulfotransferase 1) (NDST-1) (N-heparan sulfate sulfotransferase 1) (N-HSST 1) ([Heparan sulfate]-glucosamine N-sulfotransferase 1) (HSNST 1) [Includes: Heparan sulfate N-deacetylase 1 (EC 3.-.-.-); Heparan sulfate N-sulfotransferase 1 (EC 2.8.2.-)]
- Gene Names:
- NDST1 HSST HSST1
- Gene Names Primary:
- NDST1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 882
- Sequence:
- MPALACLRRLCRHVSPQAVLFLLFIFCLFSVFISAYYLYGWKRGLEPSADAPEPDCGDPPPVAPSRLLPLKPVQAATPSRTDPLVLVFVESLYSQLGQEVVAILESSRFKYRTEIAPGKGDMPTLTDKGRGRFALIIYENILKYVNLDAWNRELLDKYCVAYGVGIIGFFKANENSLLSAQLKGFPLFLHSNLGLKDCSINPKSPLLYVTRPSEVEKGVLPGEDWTVFQSNHSTYEPVLLAKTRSSESIPHLGADAGLHAALHATVVQDLGLHDGIQRVLFGNNLNFWLHKLVFVDAVAFLTGKRLSLPLDRYILVDIDDIFVGKEGTRMKVEDVKALFDTQNELRAHIPNFTFNLGYSGKFFHTGTNAEDAGDDLLLSYVKEFWWFPHMWSHMQPHLFHNQSVLAEQMALNKKFAVEHGIPTDMGYAVAPHHSGVYPVHVQLYEAWKQVWSIRVTSTEEYPHLKPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSSELDKIINGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFKHLVRFLHSWTNLRLQTLPPVQLAQKYFQIFSEEKDPLWQDPCEDKRHKDIWSKEKTCDRFPKLLIIGPQKTGTTALYLFLGMHPDLSSNYPSSETFEEIQFFNGHNYHKGIDWYMEFFPIPSNTTSDFYFEKSANYFDSEVAPRRAAALLPKAKVLTILINPADRAYSWYQHQRAHDDPVALKYTFHEVITAGSDASSKLRALQNRCLVPGWYATHIERWLSAYHANQILVLDGKLLRTEPAKVMDMVQKFLGVTNTIDYHKTLAFDPKKGFWCQLLEGGKTKCLGKSKGRKYPEMDLDSRAFLKDYYRDHNIELSKLLYKMGQTLPTWLREDLQNTR
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Golgi apparatus membrane
Function
- Function:
- Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis (PubMed:10758005, PubMed:12634318). Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response (PubMed:10758005, PubMed:12634318). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
- Pathway:
- Glycan metabolism; heparan sulfate biosynthesis.; Glycan metabolism; heparin biosynthesis.
- Catalytic Activity:
- 3'-phosphoadenylyl sulfate + -glucosamine = adenosine 3',5'-bisphosphate + -N-sulfoglucosamine.
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=13.3 uM for K5 polysaccharide
- Active Site:
- ACT_SITE 614 614 For sulfotransferase activity.
- Gene Ontology Go:
- Golgi membrane
integral component of membrane
-glucosamine N-sulfotransferase activity
deacetylase activity
aorta development
carbohydrate metabolic process
cardiac septum development
coronary vasculature development
embryonic neurocranium morphogenesis
embryonic viscerocranium morphogenesis
fibroblast growth factor receptor signaling pathway
forebrain development
glycosaminoglycan biosynthetic process
glycosaminoglycan metabolic process
heparan sulfate proteoglycan biosynthetic process
heparin biosynthetic process
inflammatory response
MAPK cascade
midbrain development
polysaccharide biosynthetic process
protein sulfation
respiratory gaseous exchange
small molecule metabolic process
smoothened signaling pathway - Gene Ontology Biological Process:
- aorta development
carbohydrate metabolic process
cardiac septum development
coronary vasculature development
embryonic neurocranium morphogenesis
embryonic viscerocranium morphogenesis
fibroblast growth factor receptor signaling pathway
forebrain development
glycosaminoglycan biosynthetic process
glycosaminoglycan metabolic process
heparan sulfate proteoglycan biosynthetic process
heparin biosynthetic process
inflammatory response
MAPK cascade
midbrain development
polysaccharide biosynthetic process
protein sulfation
respiratory gaseous exchange
small molecule metabolic process
smoothened signaling pathway - Gene Ontology Molecular Function:
- -glucosamine N-sulfotransferase activity
deacetylase activity - Gene Ontology Cellular Component:
- Golgi membrane
integral component of membrane - Keywords:
- 3D-structure
Alternative splicing
Complete proteome
Disease mutation
Disulfide bond
Glycoprotein
Golgi apparatus
Hydrolase
Inflammatory response
Membrane
Mental retardation
Multifunctional enzyme
Reference proteome
Signal-anchor
Transferase
Transmembrane
Transmembrane helix