Names & Taxonomy
- Uniprot ID:
- P51955
- Entry Name:
- NEK2_HUMAN
- Status:
- reviewed
- Protein Names:
- Serine/threonine-protein kinase Nek2 (EC 2.7.11.1) (HSPK 21) (Never in mitosis A-related kinase 2) (NimA-related protein kinase 2) (NimA-like protein kinase 1)
- Gene Names:
- NEK2 NEK2A NLK1
- Gene Names Primary:
- NEK2
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 445
- Sequence:
- MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALMPPFTAFSQKELAGKIREGKFRRIPYRYSDELNEIITRMLNLKDYHRPSVEEILENPLIADLVADEQRRNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQERERALKAREERLEQKEQELCVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Isoform 1: Nucleus. Nucleus, nucleolus
Function
- Function:
- Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143). Involved in the regulation of centrosome disjunction (PubMed:26220856).
- Catalytic Activity:
- ATP + a protein = ADP + a phosphoprotein.
- Cofactor:
- COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Enzyme Regulation:
- ENZYME REGULATION: Isoform 1 is inhibited by ionizing radiation in the presence of PPP1CA. Its catalytic activity is inhibited by the inhibitor CCT241950. In the presence of this inhibitor, displays an autoinhibited conformation: Tyr-70 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix.
- Active Site:
- ACT_SITE 141 141 Proton acceptor.
- Gene Ontology Go:
- centrosome
condensed chromosome kinetochore
condensed nuclear chromosome
cytoplasm
cytosol
kinetochore
microtubule
midbody
nucleolus
nucleus
protein complex
spindle pole
ATP binding
metal ion binding
protein kinase activity
protein phosphatase binding
protein serine/threonine kinase activity
anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
blastocyst development
cell division
centrosome separation
chromosome segregation
G2/M transition of mitotic cell cycle
meiotic cell cycle
mitotic cell cycle
mitotic nuclear division
mitotic sister chromatid segregation
mitotic spindle assembly
negative regulation of centriole-centriole cohesion
negative regulation of DNA binding
organelle organization
positive regulation of telomerase activity
positive regulation of telomere capping
positive regulation of telomere maintenance via telomerase
protein autophosphorylation
protein phosphorylation
regulation of attachment of spindle microtubules to kinetochore
regulation of mitotic centrosome separation
regulation of mitotic nuclear division
spindle assembly - Gene Ontology Biological Process:
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
blastocyst development
cell division
centrosome separation
chromosome segregation
G2/M transition of mitotic cell cycle
meiotic cell cycle
mitotic cell cycle
mitotic nuclear division
mitotic sister chromatid segregation
mitotic spindle assembly
negative regulation of centriole-centriole cohesion
negative regulation of DNA binding
organelle organization
positive regulation of telomerase activity
positive regulation of telomere capping
positive regulation of telomere maintenance via telomerase
protein autophosphorylation
protein phosphorylation
regulation of attachment of spindle microtubules to kinetochore
regulation of mitotic centrosome separation
regulation of mitotic nuclear division
spindle assembly - Gene Ontology Molecular Function:
- ATP binding
metal ion binding
protein kinase activity
protein phosphatase binding
protein serine/threonine kinase activity - Gene Ontology Cellular Component:
- centrosome
condensed chromosome kinetochore
condensed nuclear chromosome
cytoplasm
cytosol
kinetochore
microtubule
midbody
nucleolus
nucleus
protein complex
spindle pole - Keywords:
- 3D-structure
ATP-binding
Alternative splicing
Cell cycle
Cell division
Centromere
Chromosome
Chromosome partition
Coiled coil
Complete proteome
Cytoplasm
Cytoskeleton
Kinase
Kinetochore
Magnesium
Meiosis
Metal-binding
Microtubule
Mitosis
Nucleotide-binding
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
Retinitis pigmentosa
Serine/threonine-protein kinase
Transferase - Interacts With:
- Q9UJX5; P30260; Q6GQ04; Q8NG66