Names & Taxonomy

Uniprot ID:
P51580
Entry Name:
TPMT_HUMAN
Status:
reviewed
Protein Names:
Thiopurine S-methyltransferase (EC 2.1.1.67) (Thiopurine methyltransferase)
Gene Names:
TPMT
Gene Names Primary:
TPMT
Organism:
Homo sapiens (Human)

Structure

Length:
245
Sequence:
MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRCLEKVDAFEERHKSWGIDCLFEKLYLLTEK
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm.

Function

Function:
Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
Catalytic Activity:
S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=5.6 mM for S-adenosyl-L-methionine
Enzyme Regulation:
ENZYME REGULATION: Inhibited by S-adenosyl-L-homocysteine (SAH).
Cross Reference Drug Bank:
DB00993 DB00436 DB01327 DB01033 DB01250 DB01021
Gene Ontology Go:
cytosol
extracellular exosome
thiopurine S-methyltransferase activity
methylation
nucleobase-containing compound metabolic process
small molecule metabolic process
xenobiotic metabolic process
Gene Ontology Biological Process:
methylation
nucleobase-containing compound metabolic process
small molecule metabolic process
xenobiotic metabolic process
Gene Ontology Molecular Function:
thiopurine S-methyltransferase activity
Gene Ontology Cellular Component:
cytosol
extracellular exosome
Keywords:
3D-structure
Acetylation
Complete proteome
Cytoplasm
Direct protein sequencing
Methyltransferase
Phosphoprotein
Polymorphism
Reference proteome
S-adenosyl-L-methionine
Transferase

Publication

PubMed ID:
8316220 7628307 8561894 9453052 9246020 14574404 15489334 9711875 18484748 18669648 18318008 19690332 19608861 21269460 24275569 17243178 7862671 8644731 9336428 9177237 9931345 9931346 10208641 10751626 16220112 15819814 16476125 16789994