Names & Taxonomy

Uniprot ID:
P49588
Entry Name:
SYAC_HUMAN
Status:
reviewed
Protein Names:
Alanine--tRNA ligase, cytoplasmic (EC 6.1.1.7) (Alanyl-tRNA synthetase) (AlaRS) (Renal carcinoma antigen NY-REN-42)
Gene Names:
AARS
Gene Names Primary:
AARS
Organism:
Homo sapiens (Human)

Structure

Length:
968
Sequence:
MDSTLTASEIRQRFIDFFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPIERLYVTYFGGDEAAGLEADLECKQIWQNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDSKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRARGLEVTDDSPKYNYHLDSSGSYVFENTVATVMALRREKMFVEEVSTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGDLKVGDQVWLFIDEPRRRPIMSNHTATHILNFALRSVLGEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIANEMIEAAKAVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKAESLKKCLSVMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDELRETLKSLKKVMDDLDRASKADVQKRVLEKTKQFIDSNPNQPLVILEMESGASAKALNEALKLFKMHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDVSAQATGKNVGCLQEALQLATSFAQLRLGDVKN
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm

Function

Function:
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Catalytic Activity:
ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Cofactor:
COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: ; Kinetic parameters: KM=3.1 uM for tRNA(Ala) (at 37 Celsius)
Cross Reference Drug Bank:
DB00160
Gene Ontology Go:
cytoplasm
cytosol
extracellular exosome
membrane
alanine-tRNA ligase activity
amino acid binding
aminoacyl-tRNA editing activity
ATP binding
metal ion binding
tRNA binding
alanyl-tRNA aminoacylation
cerebellar Purkinje cell layer development
endoplasmic reticulum unfolded protein response
gene expression
hair follicle development
negative regulation of neuron apoptotic process
neuromuscular process controlling balance
protein folding
response to amino acid
tRNA aminoacylation for protein translation
tRNA modification
tRNA processing
Gene Ontology Biological Process:
alanyl-tRNA aminoacylation
cerebellar Purkinje cell layer development
endoplasmic reticulum unfolded protein response
gene expression
hair follicle development
negative regulation of neuron apoptotic process
neuromuscular process controlling balance
protein folding
response to amino acid
tRNA aminoacylation for protein translation
tRNA modification
tRNA processing
Gene Ontology Molecular Function:
alanine-tRNA ligase activity
amino acid binding
aminoacyl-tRNA editing activity
ATP binding
metal ion binding
tRNA binding
Gene Ontology Cellular Component:
cytoplasm
cytosol
extracellular exosome
membrane
Keywords:
3D-structure
ATP-binding
Acetylation
Alternative splicing
Aminoacyl-tRNA synthetase
Charcot-Marie-Tooth disease
Complete proteome
Cytoplasm
Direct protein sequencing
Disease mutation
Epilepsy
Ligase
Metal-binding
Neurodegeneration
Neuropathy
Nucleotide-binding
Phosphoprotein
Polymorphism
Protein biosynthesis
RNA-binding
Reference proteome
Ubl conjugation
Zinc
tRNA-binding

Publication

PubMed ID:
7654687 14702039 15616553 15489334 10508479 16139798 18669648 19413330 19661429 19608861 20068231 21269460 22223895 22814378 24275569 25817015 25944712 20045102 22206013 22009580 24627108