Names & Taxonomy
- Uniprot ID:
- P49588
- Entry Name:
- SYAC_HUMAN
- Status:
- reviewed
- Protein Names:
- Alanine--tRNA ligase, cytoplasmic (EC 6.1.1.7) (Alanyl-tRNA synthetase) (AlaRS) (Renal carcinoma antigen NY-REN-42)
- Gene Names:
- AARS
- Gene Names Primary:
- AARS
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 968
- Sequence:
- MDSTLTASEIRQRFIDFFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPIERLYVTYFGGDEAAGLEADLECKQIWQNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDSKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRARGLEVTDDSPKYNYHLDSSGSYVFENTVATVMALRREKMFVEEVSTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGDLKVGDQVWLFIDEPRRRPIMSNHTATHILNFALRSVLGEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIANEMIEAAKAVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKAESLKKCLSVMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDELRETLKSLKKVMDDLDRASKADVQKRVLEKTKQFIDSNPNQPLVILEMESGASAKALNEALKLFKMHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDVSAQATGKNVGCLQEALQLATSFAQLRLGDVKN
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm
Function
- Function:
- Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
- Catalytic Activity:
- ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
- Cofactor:
- COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: ; Kinetic parameters: KM=3.1 uM for tRNA(Ala) (at 37 Celsius)
- Cross Reference Drug Bank:
- DB00160
- Gene Ontology Go:
- cytoplasm
cytosol
extracellular exosome
membrane
alanine-tRNA ligase activity
amino acid binding
aminoacyl-tRNA editing activity
ATP binding
metal ion binding
tRNA binding
alanyl-tRNA aminoacylation
cerebellar Purkinje cell layer development
endoplasmic reticulum unfolded protein response
gene expression
hair follicle development
negative regulation of neuron apoptotic process
neuromuscular process controlling balance
protein folding
response to amino acid
tRNA aminoacylation for protein translation
tRNA modification
tRNA processing - Gene Ontology Biological Process:
- alanyl-tRNA aminoacylation
cerebellar Purkinje cell layer development
endoplasmic reticulum unfolded protein response
gene expression
hair follicle development
negative regulation of neuron apoptotic process
neuromuscular process controlling balance
protein folding
response to amino acid
tRNA aminoacylation for protein translation
tRNA modification
tRNA processing - Gene Ontology Molecular Function:
- alanine-tRNA ligase activity
amino acid binding
aminoacyl-tRNA editing activity
ATP binding
metal ion binding
tRNA binding - Gene Ontology Cellular Component:
- cytoplasm
cytosol
extracellular exosome
membrane - Keywords:
- 3D-structure
ATP-binding
Acetylation
Alternative splicing
Aminoacyl-tRNA synthetase
Charcot-Marie-Tooth disease
Complete proteome
Cytoplasm
Direct protein sequencing
Disease mutation
Epilepsy
Ligase
Metal-binding
Neurodegeneration
Neuropathy
Nucleotide-binding
Phosphoprotein
Polymorphism
Protein biosynthesis
RNA-binding
Reference proteome
Ubl conjugation
Zinc
tRNA-binding