Names & Taxonomy
- Uniprot ID:
- P49336
- Entry Name:
- CDK8_HUMAN
- Status:
- reviewed
- Protein Names:
- Cyclin-dependent kinase 8 (EC 2.7.11.22) (EC 2.7.11.23) (Cell division protein kinase 8) (Mediator complex subunit CDK8) (Mediator of RNA polymerase II transcription subunit CDK8) (Protein kinase K35)
- Gene Names:
- CDK8
- Gene Names Primary:
- CDK8
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 464
- Sequence:
- MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQIEGTGISMSACREIALLRELKHPNVISLQKVFLSHADRKVWLLFDYAEHDLWHIIKFHRASKANKKPVQLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPYHHDQLDRIFNVMGFPADKDWEDIKKMPEHSTLMKDFRRNTYTNCSLIKYMEKHKVKPDSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLEDPLPTSDVFAGCQIPYPKREFLTEEEPDDKGDKKNQQQQQGNNHTNGTGHPGNQDSSHTQGPPLKKVRVVPPTTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSSMGYSATSQQPPQYSHQTHRY
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Nucleus
Function
- Function:
- Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
- Catalytic Activity:
- ATP + a protein = ADP + a phosphoprotein.
- Cofactor:
- COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Active Site:
- ACT_SITE 151 151 Proton acceptor.
- Gene Ontology Go:
- mediator complex
nucleoplasm
nucleus
ATP binding
cyclin-dependent protein serine/threonine kinase activity
protein kinase activity
RNA polymerase II carboxy-terminal domain kinase activity
gene expression
Notch signaling pathway
positive regulation of transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription, DNA-templated
transforming growth factor beta receptor signaling pathway - Gene Ontology Biological Process:
- gene expression
Notch signaling pathway
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
transforming growth factor beta receptor signaling pathway - Gene Ontology Molecular Function:
- ATP binding
cyclin-dependent protein serine/threonine kinase activity
protein kinase activity
RNA polymerase II carboxy-terminal domain kinase activity - Gene Ontology Cellular Component:
- mediator complex
nucleoplasm
nucleus - Keywords:
- 3D-structure
ATP-binding
Activator
Alternative splicing
Complete proteome
Kinase
Nucleotide-binding
Nucleus
Polymorphism
Reference proteome
Repressor
Serine/threonine-protein kinase
Transcription
Transcription regulation
Transferase - Interacts With:
- P24863