Names & Taxonomy

Uniprot ID:
P49336
Entry Name:
CDK8_HUMAN
Status:
reviewed
Protein Names:
Cyclin-dependent kinase 8 (EC 2.7.11.22) (EC 2.7.11.23) (Cell division protein kinase 8) (Mediator complex subunit CDK8) (Mediator of RNA polymerase II transcription subunit CDK8) (Protein kinase K35)
Gene Names:
CDK8
Gene Names Primary:
CDK8
Organism:
Homo sapiens (Human)

Structure

Length:
464
Sequence:
MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQIEGTGISMSACREIALLRELKHPNVISLQKVFLSHADRKVWLLFDYAEHDLWHIIKFHRASKANKKPVQLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPYHHDQLDRIFNVMGFPADKDWEDIKKMPEHSTLMKDFRRNTYTNCSLIKYMEKHKVKPDSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLEDPLPTSDVFAGCQIPYPKREFLTEEEPDDKGDKKNQQQQQGNNHTNGTGHPGNQDSSHTQGPPLKKVRVVPPTTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSSMGYSATSQQPPQYSHQTHRY
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Nucleus

Function

Function:
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
Catalytic Activity:
ATP + a protein = ADP + a phosphoprotein.
Cofactor:
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Active Site:
ACT_SITE 151 151 Proton acceptor.
Gene Ontology Go:
mediator complex
nucleoplasm
nucleus
ATP binding
cyclin-dependent protein serine/threonine kinase activity
protein kinase activity
RNA polymerase II carboxy-terminal domain kinase activity
gene expression
Notch signaling pathway
positive regulation of transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription, DNA-templated
transforming growth factor beta receptor signaling pathway
Gene Ontology Biological Process:
gene expression
Notch signaling pathway
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
transforming growth factor beta receptor signaling pathway
Gene Ontology Molecular Function:
ATP binding
cyclin-dependent protein serine/threonine kinase activity
protein kinase activity
RNA polymerase II carboxy-terminal domain kinase activity
Gene Ontology Cellular Component:
mediator complex
nucleoplasm
nucleus
Keywords:
3D-structure
ATP-binding
Activator
Alternative splicing
Complete proteome
Kinase
Nucleotide-binding
Nucleus
Polymorphism
Reference proteome
Repressor
Serine/threonine-protein kinase
Transcription
Transcription regulation
Transferase
Interacts With:
P24863

Publication

PubMed ID:
7568034 15057823 15489334 9734358 10024883 10993082 15546612 15175163 15989967 17000779 19369195 21806996 17344846