Names & Taxonomy

Uniprot ID:
P46459
Entry Name:
NSF_HUMAN
Status:
reviewed
Protein Names:
Vesicle-fusing ATPase (EC 3.6.4.6) (N-ethylmaleimide-sensitive fusion protein) (NEM-sensitive fusion protein) (Vesicular-fusion protein NSF)
Gene Names:
NSF
Gene Names Primary:
NSF
Organism:
Homo sapiens (Human)

Structure

Length:
744
Sequence:
MAGRSMQAARCPTDELSLTNCAVVNEKDFQSGQHVIVRTSPNHRYTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASPLDFD
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm.

Function

Function:
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).
Catalytic Activity:
ATP + H(2)O = ADP + phosphate.
Cofactor:
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Gene Ontology Go:
cytoplasm
cytosol
dendritic shaft
extracellular exosome
Golgi membrane
Golgi stack
lysosomal membrane
myelin sheath
plasma membrane
postsynaptic density
ATP binding
ATPase activity
ATPase activity, coupled
metal ion binding
PDZ domain binding
protein complex binding
protein kinase binding
Rab GTPase binding
syntaxin-1 binding
cellular protein metabolic process
COPII vesicle coating
ER to Golgi vesicle-mediated transport
exocytosis
Golgi to plasma membrane protein transport
Golgi vesicle docking
intra-Golgi vesicle-mediated transport
intracellular protein transport
membrane organization
plasma membrane fusion
positive regulation of protein catabolic process
positive regulation of receptor recycling
post-translational protein modification
potassium ion transport
protein N-linked glycosylation via asparagine
regulation of exocytosis
synaptic transmission
vesicle-mediated transport
Gene Ontology Biological Process:
cellular protein metabolic process
COPII vesicle coating
ER to Golgi vesicle-mediated transport
exocytosis
Golgi to plasma membrane protein transport
Golgi vesicle docking
intracellular protein transport
intra-Golgi vesicle-mediated transport
membrane organization
plasma membrane fusion
positive regulation of protein catabolic process
positive regulation of receptor recycling
post-translational protein modification
potassium ion transport
protein N-linked glycosylation via asparagine
regulation of exocytosis
synaptic transmission
vesicle-mediated transport
Gene Ontology Molecular Function:
ATPase activity
ATPase activity, coupled
ATP binding
metal ion binding
PDZ domain binding
protein complex binding
protein kinase binding
Rab GTPase binding
syntaxin-1 binding
Gene Ontology Cellular Component:
cytoplasm
cytosol
dendritic shaft
extracellular exosome
Golgi membrane
Golgi stack
lysosomal membrane
myelin sheath
plasma membrane
postsynaptic density
Keywords:
ATP-binding
Acetylation
Alternative splicing
Complete proteome
Cytoplasm
Hydrolase
Magnesium
Metal-binding
Nucleotide-binding
Phosphoprotein
Polymorphism
Protein transport
Reference proteome
Repeat
Transport
Interacts With:
Q9UBS5; O75899; P42262; P54920; P43378

Publication

PubMed ID:
14702039 16625196 15489334 21269460 24275569 25944712