Names & Taxonomy

Uniprot ID:
P42574
Entry Name:
CASP3_HUMAN
Status:
reviewed
Protein Names:
Caspase-3 (CASP-3) (EC 3.4.22.56) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]
Gene Names:
CASP3 CPP32
Gene Names Primary:
CASP3
Organism:
Homo sapiens (Human)

Structure

Length:
277
Sequence:
MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm.

Function

Function:
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Catalytic Activity:
Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.
Enzyme Regulation:
ENZYME REGULATION: Inhibited by isatin sulfonamides.
Active Site:
ACT_SITE 121 121
Cross Reference Drug Bank:
DB01017
Gene Ontology Go:
cytosol
death-inducing signaling complex
membrane raft
nucleoplasm
nucleus
plasma membrane
aspartic-type endopeptidase activity
cyclin-dependent protein serine/threonine kinase inhibitor activity
cysteine-type endopeptidase activity
cysteine-type endopeptidase activity involved in apoptotic process
cysteine-type endopeptidase activity involved in execution phase of apoptosis
peptidase activity
phospholipase A2 activator activity
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
apoptotic DNA fragmentation
apoptotic process
apoptotic signaling pathway
B cell homeostasis
cell fate commitment
cellular component disassembly involved in execution phase of apoptosis
cellular response to DNA damage stimulus
cellular response to organic cyclic compound
erythrocyte differentiation
execution phase of apoptosis
extracellular matrix disassembly
extracellular matrix organization
extrinsic apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
glial cell apoptotic process
heart development
hippo signaling
hippocampus development
intrinsic apoptotic signaling pathway
keratinocyte differentiation
learning or memory
negative regulation of activated T cell proliferation
negative regulation of apoptotic process
negative regulation of B cell proliferation
negative regulation of cyclin-dependent protein serine/threonine kinase activity
neuron apoptotic process
neuron differentiation
neurotrophin TRK receptor signaling pathway
platelet formation
positive regulation of neuron apoptotic process
programmed cell death
proteolysis
regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of macroautophagy
response to amino acid
response to antibiotic
response to cobalt ion
response to drug
response to estradiol
response to glucocorticoid
response to glucose
response to hydrogen peroxide
response to hypoxia
response to lipopolysaccharide
response to nicotine
response to tumor necrosis factor
response to UV
response to X-ray
sensory perception of sound
T cell homeostasis
wound healing
Gene Ontology Biological Process:
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
apoptotic DNA fragmentation
apoptotic process
apoptotic signaling pathway
B cell homeostasis
cell fate commitment
cellular component disassembly involved in execution phase of apoptosis
cellular response to DNA damage stimulus
cellular response to organic cyclic compound
erythrocyte differentiation
execution phase of apoptosis
extracellular matrix disassembly
extracellular matrix organization
extrinsic apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
glial cell apoptotic process
heart development
hippocampus development
hippo signaling
intrinsic apoptotic signaling pathway
keratinocyte differentiation
learning or memory
negative regulation of activated T cell proliferation
negative regulation of apoptotic process
negative regulation of B cell proliferation
negative regulation of cyclin-dependent protein serine/threonine kinase activity
neuron apoptotic process
neuron differentiation
neurotrophin TRK receptor signaling pathway
platelet formation
positive regulation of neuron apoptotic process
programmed cell death
proteolysis
regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of macroautophagy
response to amino acid
response to antibiotic
response to cobalt ion
response to drug
response to estradiol
response to glucocorticoid
response to glucose
response to hydrogen peroxide
response to hypoxia
response to lipopolysaccharide
response to nicotine
response to tumor necrosis factor
response to UV
response to X-ray
sensory perception of sound
T cell homeostasis
wound healing
Gene Ontology Molecular Function:
aspartic-type endopeptidase activity
cyclin-dependent protein serine/threonine kinase inhibitor activity
cysteine-type endopeptidase activity
cysteine-type endopeptidase activity involved in apoptotic process
cysteine-type endopeptidase activity involved in execution phase of apoptosis
peptidase activity
phospholipase A2 activator activity
Gene Ontology Cellular Component:
cytosol
death-inducing signaling complex
membrane raft
nucleoplasm
nucleus
plasma membrane
Keywords:
3D-structure
Acetylation
Apoptosis
Complete proteome
Cytoplasm
Direct protein sequencing
Hydrolase
Phosphoprotein
Polymorphism
Protease
Reference proteome
S-nitrosylation
Thiol protease
Zymogen
Interacts With:
O43823; Q9Y243; Q00987; Q9BYP7; P98170

Publication

PubMed ID:
7983002 7774019 15003516 14702039 15489334 7596430 8755496 8696339 10213689 15200957 19413330 21269460 21357690 22223895 8673606 9045680 10821855