Names & Taxonomy
- Uniprot ID:
- P34897
- Entry Name:
- GLYM_HUMAN
- Status:
- reviewed
- Protein Names:
- Serine hydroxymethyltransferase, mitochondrial (SHMT) (EC 2.1.2.1) (Glycine hydroxymethyltransferase) (Serine methylase)
- Gene Names:
- SHMT2
- Gene Names Primary:
- SHMT2
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 504
- Sequence:
- MLYFSLFWAARPLQRCGQLVRMAIRAQHSNAAQTQTGEANRGWTGQESLSDSDPEMWELLQREKDRQCRGLELIASENFCSRAALEALGSCLNNKYSEGYPGKRYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQPYSGSPANLAVYTALLQPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPYKLNPKTGLIDYNQLALTARLFRPRLIIAGTSAYARLIDYARMREVCDEVKAHLLADMAHISGLVAAKVIPSPFKHADIVTTTTHKTLRGARSGLIFYRKGVKAVDPKTGREIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREYSLQVLKNARAMADALLERGYSLVSGGTDNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKSKTAKLQDFKSFLLKDSETSQRLANLRQRVEQFARAFPMPGFDEH
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Mitochondrion
Function
- Function:
- Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism. Thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed:21876188). Interconversion of serine and glycine (PubMed:25619277). Associates with mitochondrial DNA (PubMed:18063578). Plays a role in the deubiquitination of target proteins as component of the BRISC complex (PubMed:24075985). Required for IFNAR1 deubiquitination by the BRISC complex (PubMed:24075985).
- Pathway:
- One-carbon metabolism; tetrahydrofolate interconversion.
- Catalytic Activity:
- 5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine.
- Cofactor:
- COFACTOR: Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=278 uM for L-serine
- Cross Reference Drug Bank:
- DB00145 DB00116
- Gene Ontology Go:
- BRISC complex
cytoplasm
extracellular exosome
microtubule cytoskeleton
mitochondrial inner membrane
mitochondrial intermembrane space
mitochondrial matrix
mitochondrial nucleoid
mitochondrion
nucleus
amino acid binding
chromatin binding
glycine hydroxymethyltransferase activity
L-allo-threonine aldolase activity
pyridoxal phosphate binding
folic acid metabolic process
glycine biosynthetic process from serine
glycine metabolic process
L-serine biosynthetic process
L-serine metabolic process
one-carbon metabolic process
positive regulation of cell proliferation
protein homotetramerization
protein K63-linked deubiquitination
protein tetramerization
response to type I interferon
small molecule metabolic process
tetrahydrofolate interconversion
tetrahydrofolate metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process - Gene Ontology Biological Process:
- folic acid metabolic process
glycine biosynthetic process from serine
glycine metabolic process
L-serine biosynthetic process
L-serine metabolic process
one-carbon metabolic process
positive regulation of cell proliferation
protein homotetramerization
protein K63-linked deubiquitination
protein tetramerization
response to type I interferon
small molecule metabolic process
tetrahydrofolate interconversion
tetrahydrofolate metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process - Gene Ontology Molecular Function:
- amino acid binding
chromatin binding
glycine hydroxymethyltransferase activity
L-allo-threonine aldolase activity
pyridoxal phosphate binding - Gene Ontology Cellular Component:
- BRISC complex
cytoplasm
extracellular exosome
microtubule cytoskeleton
mitochondrial inner membrane
mitochondrial intermembrane space
mitochondrial matrix
mitochondrial nucleoid
mitochondrion
nucleus - Keywords:
- 3D-structure
Acetylation
Alternative splicing
Complete proteome
Cytoplasm
Membrane
Mitochondrion
Mitochondrion inner membrane
Mitochondrion nucleoid
Nucleus
One-carbon metabolism
Pyridoxal phosphate
Reference proteome
Transferase
Transit peptide - Interacts With:
- Q15041; Q96DZ9