Names & Taxonomy

Uniprot ID:
P26439
Entry Name:
3BHS2_HUMAN
Status:
reviewed
Protein Names:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II) (3-beta-HSD II) (3-beta-HSD adrenal and gonadal type) [Includes: 3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC 1.1.1.145) (3-beta-hydroxy-5-ene steroid dehydrogenase) (Progesterone reductase); Steroid Delta-isomerase (EC 5.3.3.1) (Delta-5-3-ketosteroid isomerase)]
Gene Names:
HSD3B2 HSDB3B
Gene Names Primary:
HSD3B2
Organism:
Homo sapiens (Human)

Structure

Length:
372
Sequence:
MGWSCLVTGAGGLLGQRIVRLLVEEKELKEIRALDKAFRPELREEFSKLQNRTKLTVLEGDILDEPFLKRACQDVSVVIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPTPYPYSKKLAEKAVLAANGWNLKNGDTLYTCALRPTYIYGEGGPFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALRDPKKAPSVRGQFYYISDDTPHQSYDNLNYILSKEFGLRLDSRWSLPLTLMYWIGFLLEVVSFLLSPIYSYQPPFNRHTVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKETLKSKTQ
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.

Function

Function:
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Pathway:
Lipid metabolism; steroid biosynthesis.
Catalytic Activity:
A 3-beta-hydroxy-Delta(5)-steroid + NAD(+) = a 3-oxo-Delta(5)-steroid + NADH.; A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Active Site:
ACT_SITE 154 154 Proton acceptor.
Cross Reference Drug Bank:
DB01285 DB00603 DB01108
Gene Ontology Go:
endoplasmic reticulum
endoplasmic reticulum membrane
integral component of membrane
mitochondrial inner membrane
mitochondrial intermembrane space
mitochondrial membrane
smooth endoplasmic reticulum membrane
3-beta-hydroxy-delta5-steroid dehydrogenase activity
steroid delta-isomerase activity
androgen biosynthetic process
glucocorticoid biosynthetic process
mineralocorticoid biosynthetic process
small molecule metabolic process
steroid biosynthetic process
steroid metabolic process
Gene Ontology Biological Process:
androgen biosynthetic process
glucocorticoid biosynthetic process
mineralocorticoid biosynthetic process
small molecule metabolic process
steroid biosynthetic process
steroid metabolic process
Gene Ontology Molecular Function:
3-beta-hydroxy-delta5-steroid dehydrogenase activity
steroid delta-isomerase activity
Gene Ontology Cellular Component:
endoplasmic reticulum
endoplasmic reticulum membrane
integral component of membrane
mitochondrial inner membrane
mitochondrial intermembrane space
mitochondrial membrane
smooth endoplasmic reticulum membrane
Keywords:
Alternative splicing
Complete proteome
Congenital adrenal hyperplasia
Disease mutation
Endoplasmic reticulum
Isomerase
Membrane
Mitochondrion
Multifunctional enzyme
NAD
Oxidoreductase
Polymorphism
Reference proteome
Steroidogenesis
Transmembrane
Transmembrane helix

Publication

PubMed ID:
1741954 1944309 17974005 16710414 15489334 7588414 1363812 14764797 8316254 7833923 8126127 7962268 8185809 8060486 7893703 7633426 7633460 7608265 9719627 10599696 10651755 10843183 12050213 18252794 22579964