Names & Taxonomy
- Uniprot ID:
- P22314
- Entry Name:
- UBA1_HUMAN
- Status:
- reviewed
- Protein Names:
- Ubiquitin-like modifier-activating enzyme 1 (EC 6.2.1.45) (Protein A1S9) (Ubiquitin-activating enzyme E1)
- Gene Names:
- UBA1 A1S9T UBE1
- Gene Names Primary:
- UBA1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 1058
- Sequence:
- MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm
Function
- Function:
- Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).
- Pathway:
- Protein modification; protein ubiquitination.
- Catalytic Activity:
- ATP + ubiquitin + -L-cysteine = AMP + diphosphate + S-ubiquitinyl--L-cysteine.
- Active Site:
- ACT_SITE 632 632 Glycyl thioester intermediate.
- Gene Ontology Go:
- cytoplasm
cytosol
extracellular exosome
mitochondrion
nucleus
ATP binding
poly(A) RNA binding
ubiquitin activating enzyme activity
ubiquitin-protein transferase activity
cellular response to DNA damage stimulus
modification-dependent protein catabolic process - Gene Ontology Biological Process:
- cellular response to DNA damage stimulus
modification-dependent protein catabolic process - Gene Ontology Molecular Function:
- ATP binding
poly(A) RNA binding
ubiquitin activating enzyme activity
ubiquitin-protein transferase activity - Gene Ontology Cellular Component:
- cytoplasm
cytosol
extracellular exosome
mitochondrion
nucleus - Keywords:
- 3D-structure
ATP-binding
Acetylation
Alternative initiation
Complete proteome
Cytoplasm
Direct protein sequencing
Disease mutation
Ligase
Mitochondrion
Neurodegeneration
Nucleotide-binding
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
Repeat
Ubl conjugation
Ubl conjugation pathway - Interacts With:
- Q76353; Q9H2C0; P63279