Names & Taxonomy
- Uniprot ID:
- P14060
- Entry Name:
- 3BHS1_HUMAN
- Status:
- reviewed
- Protein Names:
- 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I) (3-beta-HSD I) (Trophoblast antigen FDO161G) [Includes: 3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC 1.1.1.145) (3-beta-hydroxy-5-ene steroid dehydrogenase) (Progesterone reductase); Steroid Delta-isomerase (EC 5.3.3.1) (Delta-5-3-ketosteroid isomerase)]
- Gene Names:
- HSD3B1 3BH HSDB3A
- Gene Names Primary:
- HSD3B1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 373
- Sequence:
- MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVLDKAFGPELREEFSKLQNKTKLTVLEGDILDEPFLKRACQDVSVIIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPAPYPHSKKLAEKAVLAANGWNLKNGGTLYTCALRPMYIYGEGSRFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALQDPKKAPSIRGQFYYISDDTPHQSYDNLNYTLSKEFGLRLDSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPFNRHIVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKETLKSKTQ
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Function
- Function:
- 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.
- Pathway:
- Lipid metabolism; steroid biosynthesis.
- Catalytic Activity:
- A 3-beta-hydroxy-Delta(5)-steroid + NAD(+) = a 3-oxo-Delta(5)-steroid + NADH.; A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
- Active Site:
- ACT_SITE 155 155 Proton acceptor.
- Cross Reference Drug Bank:
- DB01108
- Gene Ontology Go:
- endoplasmic reticulum membrane
integral component of membrane
mitochondrial inner membrane
mitochondrial intermembrane space
smooth endoplasmic reticulum membrane
3-beta-hydroxy-delta5-steroid dehydrogenase activity
steroid delta-isomerase activity
androgen biosynthetic process
estrogen biosynthetic process
glucocorticoid biosynthetic process
mineralocorticoid biosynthetic process
small molecule metabolic process
steroid biosynthetic process
steroid metabolic process - Gene Ontology Biological Process:
- androgen biosynthetic process
estrogen biosynthetic process
glucocorticoid biosynthetic process
mineralocorticoid biosynthetic process
small molecule metabolic process
steroid biosynthetic process
steroid metabolic process - Gene Ontology Molecular Function:
- 3-beta-hydroxy-delta5-steroid dehydrogenase activity
steroid delta-isomerase activity - Gene Ontology Cellular Component:
- endoplasmic reticulum membrane
integral component of membrane
mitochondrial inner membrane
mitochondrial intermembrane space
smooth endoplasmic reticulum membrane - Keywords:
- Complete proteome
Direct protein sequencing
Endoplasmic reticulum
Isomerase
Membrane
Mitochondrion
Multifunctional enzyme
NAD
Oxidoreductase
Polymorphism
Reference proteome
Steroidogenesis
Transmembrane
Transmembrane helix