Names & Taxonomy

Uniprot ID:
P11712
Entry Name:
CP2C9_HUMAN
Status:
reviewed
Protein Names:
Cytochrome P450 2C9 (EC 1.14.13.-) ((R)-limonene 6-monooxygenase) (EC 1.14.13.80) ((S)-limonene 6-monooxygenase) (EC 1.14.13.48) ((S)-limonene 7-monooxygenase) (EC 1.14.13.49) (CYPIIC9) (Cholesterol 25-hydroxylase) (EC 1.14.99.38) (Cytochrome P-450MP) (Cytochrome P450 MP-4) (Cytochrome P450 MP-8) (Cytochrome P450 PB-1) (S-mephenytoin 4-hydroxylase)
Gene Names:
CYP2C9 CYP2C10
Gene Names Primary:
CYP2C9
Organism:
Homo sapiens (Human)

Structure

Length:
490
Sequence:
MDSLVVLVLCLSCLLLLSLWRQSSGRGKLPPGPTPLPVIGNILQIGIKDISKSLTNLSKVYGPVFTLYFGLKPIVVLHGYEAVKEALIDLGEEFSGRGIFPLAERANRGFGIVFSNGKKWKEIRRFSLMTLRNFGMGKRSIEDRVQEEARCLVEELRKTKASPCDPTFILGCAPCNVICSIIFHKRFDYKDQQFLNLMEKLNENIKILSSPWIQICNNFSPIIDYFPGTHNKLLKNVAFMKSYILEKVKEHQESMDMNNPQDFIDCFLMKMEKEKHNQPSEFTIESLENTAVDLFGAGTETTSTTLRYALLLLLKHPEVTAKVQEEIERVIGRNRSPCMQDRSHMPYTDAVVHEVQRYIDLLPTSLPHAVTCDIKFRNYLIPKGTTILISLTSVLHDNKEFPNPEMFDPHHFLDEGGNFKKSKYFMPFSAGKRICVGEALAGMELFLFLTSILQNFNLKSLVDPKNLDTTPVVNGFASVPPFYQLCFIPV
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane

Function

Function:
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics (PubMed:25994031). This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan (PubMed:25994031).
Catalytic Activity:
(+)-(R)-limonene + NADPH + O(2) = (+)-trans-carveol + NADP(+) + H(2)O.
Cofactor:
COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413;
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.25 mM for S-mephenytoin
Cross Reference Drug Bank:
DB01418 DB00316 DB00945 DB06594 DB00969 DB00404 DB01424 DB01118 DB00321 DB00381 DB00613 DB00701 DB01217 DB01435 DB06605 DB00673 DB01274 DB06413 DB06697 DB01072 DB01076 DB01117 DB00972 DB00307 DB01128 DB00188 DB00559 DB00835 DB00921 DB01156 DB08875 DB00201 DB00796 DB01101 DB00564 DB00748 DB01136 DB00482 DB00446 DB00672 DB00169 DB00501 DB00568 DB00604 DB00515 DB04920 DB00758 DB00257 DB00363 DB00907 DB01394 DB01176 DB00531 DB00091 DB06292 DB00250 DB00705 DB00967 DB01234 DB01191 DB00514 DB00647 DB00829 DB00586 DB01144 DB00266 DB00255 DB00343 DB01075 DB00822 DB00757 DB00843 DB00988 DB00869 DB01142 DB00470 DB00625 DB00216 DB08899 DB00668 DB01240 DB00876 DB00783 DB00655 DB04574 DB00402 DB00898 DB00749 DB01628 DB06414 DB01023 DB01039 DB01195 DB00196 DB04841 DB01544 DB00544 DB00472 DB00712 DB01095 DB00176 DB00983 DB01320 DB00317 DB01241 DB01381 DB01120 DB00222 DB01067 DB01016 DB01018 DB00502 DB01159 DB01355 DB00667 DB00062 DB00327 DB01050 DB01177 DB01181 DB00619 DB00224 DB00328 DB01029 DB00951 DB09570 DB01221 DB06738 DB01026 DB01009 DB00448 DB01097 DB00281 DB01601 DB00455 DB06725 DB00678 DB00227 DB01283 DB00603 DB00784 DB01065 DB00814 DB01357 DB00333 DB00703 DB00763 DB01028 DB00916 DB01110 DB00370 DB01171 DB00745 DB00471 DB00788 DB00731 DB00220 DB09048 DB00238 DB00622 DB06803 DB00184 DB01115 DB04868 DB00665 DB06712 DB00540 DB00334 DB09080 DB00338 DB00904 DB04938 DB00991 DB01229 DB00213 DB00617 DB08439 DB00715 DB00850 DB01174 DB00946 DB00191 DB00812 DB00252 DB01132 DB00554 DB08860 DB01411 DB06209 DB00175 DB00794 DB01032 DB00396 DB01131 DB00420 DB01069 DB01182 DB00818 DB00205 DB01589 DB00908 DB00468 DB01129 DB08896 DB01045 DB01201 DB00503 DB00412 DB01098 DB00936 DB01232 DB00418 DB01037 DB01104 DB00203 DB00641 DB06268 DB00398 DB06820 DB00359 DB06150 DB00576 DB01015 DB08798 DB00259 DB06729 DB00891 DB01138 DB00263 DB00870 DB00675 DB06204 DB00231 DB00444 DB00469 DB00857 DB00624 DB01041 DB00277 DB01154 DB00679 DB00208 DB01007 DB01124 DB00323 DB01036 DB00214 DB05109 DB00752 DB00374 DB00755 DB00897 DB00347 DB00440 DB00726 DB01361 DB00313 DB00177 DB00285 DB00661 DB08828 DB00582 DB09068 DB00682 DB04898 DB00549 DB00943 DB00495 DB00744 DB00425 DB01198
Gene Ontology Go:
endoplasmic reticulum membrane
intracellular membrane-bounded organelle
(R)-limonene 6-monooxygenase activity
(S)-limonene 6-monooxygenase activity
(S)-limonene 7-monooxygenase activity
arachidonic acid epoxygenase activity
caffeine oxidase activity
cholesterol 25-hydroxylase activity
drug binding
heme binding
iron ion binding
monooxygenase activity
oxidoreductase activity
steroid hydroxylase activity
arachidonic acid metabolic process
cellular amide metabolic process
drug catabolic process
drug metabolic process
epoxygenase P450 pathway
exogenous drug catabolic process
monocarboxylic acid metabolic process
monoterpenoid metabolic process
omega-hydroxylase P450 pathway
oxidation-reduction process
oxidative demethylation
small molecule metabolic process
steroid metabolic process
urea metabolic process
xenobiotic metabolic process
Gene Ontology Biological Process:
arachidonic acid metabolic process
cellular amide metabolic process
drug catabolic process
drug metabolic process
epoxygenase P450 pathway
exogenous drug catabolic process
monocarboxylic acid metabolic process
monoterpenoid metabolic process
omega-hydroxylase P450 pathway
oxidation-reduction process
oxidative demethylation
small molecule metabolic process
steroid metabolic process
urea metabolic process
xenobiotic metabolic process
Gene Ontology Molecular Function:
(R)-limonene 6-monooxygenase activity
(S)-limonene 6-monooxygenase activity
(S)-limonene 7-monooxygenase activity
arachidonic acid epoxygenase activity
caffeine oxidase activity
cholesterol 25-hydroxylase activity
drug binding
heme binding
iron ion binding
monooxygenase activity
oxidoreductase activity
steroid hydroxylase activity
Gene Ontology Cellular Component:
endoplasmic reticulum membrane
intracellular membrane-bounded organelle
Keywords:
3D-structure
Acetylation
Alternative splicing
Complete proteome
Direct protein sequencing
Endoplasmic reticulum
Heme
Iron
Lipid metabolism
Membrane
Metal-binding
Microsome
Monooxygenase
NADP
Oxidoreductase
Polymorphism
Reference proteome
Steroid metabolism
Sterol metabolism

Publication

PubMed ID:
2827463 3697070 14702039 15164054 15489334 2963808 3032244 3196692 1445376 3079764 3243766 1857342 8809086 8215449 11950794 21576599 24275569 12861225 8946475 9110362 10739176 11455026 11926893 15469410 21451434 23582453 24956244 25994031