Names & Taxonomy
- Uniprot ID:
- P05177
- Entry Name:
- CP1A2_HUMAN
- Status:
- reviewed
- Protein Names:
- Cytochrome P450 1A2 (EC 1.14.14.1) (CYPIA2) (Cholesterol 25-hydroxylase) (Cytochrome P(3)450) (Cytochrome P450 4) (Cytochrome P450-P3)
- Gene Names:
- CYP1A2
- Gene Names Primary:
- CYP1A2
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 515
- Sequence:
- MALSQSVPFSATELLLASAIFCLVFWVLKGLRPRVPKGLKSPPEPWGWPLLGHVLTLGKNPHLALSRMSQRYGDVLQIRIGSTPVLVLSRLDTIRQALVRQGDDFKGRPDLYTSTLITDGQSLTFSTDSGPVWAARRRLAQNALNTFSIASDPASSSSCYLEEHVSKEAKALISRLQELMAGPGHFDPYNQVVVSVANVIGAMCFGQHFPESSDEMLSLVKNTHEFVETASSGNPLDFFPILRYLPNPALQRFKAFNQRFLWFLQKTVQEHYQDFDKNSVRDITGALFKHSKKGPRASGNLIPQEKIVNLVNDIFGAGFDTVTTAISWSLMYLVTKPEIQRKIQKELDTVIGRERRPRLSDRPQLPYLEAFILETFRHSSFLPFTIPHSTTRDTTLNGFYIPKKCCVFVNQWQVNHDPELWEDPSEFRPERFLTADGTAINKPLSEKMMLFGMGKRRCIGEVLAKWEIFLFLAILLQQLEFSVPPGVKVDLTPIYGLTMKHARCEHVQARRFSIN
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane
Function
- Function:
- Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.
- Catalytic Activity:
- RH + + O(2) = ROH + + H(2)O.
- Cofactor:
- COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=4 uM for 2-amino-6-methyldipyridoimidazole
- Cross Reference Drug Bank:
- DB05812 DB01418 DB00316 DB06594 DB00518 DB00523 DB00918 DB00969 DB00357 DB01424 DB01223 DB01118 DB00321 DB00381 DB00261 DB01217 DB01435 DB06605 DB00673 DB06413 DB06216 DB00572 DB06626 DB00972 DB00207 DB06770 DB00195 DB00188 DB01558 DB01200 DB00297 DB00921 DB01156 DB00201 DB06774 DB00564 DB00262 DB01136 DB00477 DB00356 DB01166 DB00501 DB01012 DB00568 DB00537 DB00604 DB00215 DB01211 DB01407 DB04920 DB01013 DB00882 DB01242 DB00575 DB00758 DB00257 DB00363 DB00286 DB00924 DB00851 DB06292 DB01254 DB00694 DB00705 DB01151 DB00967 DB01191 DB00633 DB00829 DB00586 DB00917 DB01075 DB01184 DB00988 DB01142 DB00476 DB00974 DB00625 DB06210 DB00467 DB00668 DB00696 DB00530 DB00199 DB00783 DB00655 DB04574 DB00898 DB00773 DB01628 DB04841 DB01544 DB00544 DB00472 DB00623 DB00499 DB01095 DB00176 DB00998 DB01241 DB00400 DB00629 DB00502 DB01094 DB01355 DB04946 DB00619 DB00458 DB00724 DB05278 DB01306 DB01307 DB00047 DB01309 DB00046 DB00030 DB01029 DB00951 DB09570 DB01026 DB00448 DB01097 DB01002 DB01137 DB00281 DB00978 DB01601 DB04871 DB00678 DB01283 DB00772 DB00934 DB00737 DB01065 DB00170 DB00333 DB00763 DB01403 DB00553 DB01028 DB00379 DB06148 DB01110 DB00370 DB01171 DB00745 DB00461 DB00607 DB00788 DB00220 DB00238 DB06803 DB00184 DB01115 DB06712 DB00401 DB00435 DB00325 DB00368 DB01059 DB00540 DB01165 DB00334 DB00338 DB00904 DB09330 DB00526 DB01303 DB06412 DB00377 DB00213 DB06589 DB00487 DB00738 DB00806 DB00850 DB01174 DB00191 DB00388 DB00397 DB01100 DB01621 DB08910 DB01058 DB01087 DB00794 DB00396 DB01131 DB00420 DB01182 DB00818 DB00571 DB00339 DB00908 DB00468 DB01129 DB00980 DB00863 DB01367 DB00615 DB01045 DB08864 DB00740 DB00503 DB00953 DB00268 DB00296 DB00412 DB05271 DB00778 DB00418 DB01037 DB01104 DB06290 DB00398 DB00428 DB06820 DB00605 DB00675 DB00976 DB00300 DB00857 DB01041 DB01412 DB00277 DB00730 DB00679 DB01623 DB00208 DB01007 DB00697 DB01056 DB00539 DB00752 DB00384 DB00831 DB08867 DB00313 DB08881 DB00661 DB00682 DB00549 DB00744 DB00246 DB00315 DB00425
- Gene Ontology Go:
- endoplasmic reticulum membrane
intracellular membrane-bounded organelle
aromatase activity
caffeine oxidase activity
demethylase activity
electron carrier activity
enzyme binding
heme binding
iron ion binding
monooxygenase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
alkaloid metabolic process
arachidonic acid metabolic process
cellular respiration
cellular response to cadmium ion
dibenzo-p-dioxin metabolic process
drug catabolic process
drug metabolic process
epoxygenase P450 pathway
exogenous drug catabolic process
heterocycle metabolic process
hydrogen peroxide biosynthetic process
lung development
methylation
monocarboxylic acid metabolic process
monoterpenoid metabolic process
omega-hydroxylase P450 pathway
oxidation-reduction process
oxidative deethylation
oxidative demethylation
porphyrin-containing compound metabolic process
post-embryonic development
regulation of gene expression
response to estradiol
response to immobilization stress
response to lipopolysaccharide
small molecule metabolic process
steroid catabolic process
toxin biosynthetic process
xenobiotic metabolic process - Gene Ontology Biological Process:
- alkaloid metabolic process
arachidonic acid metabolic process
cellular respiration
cellular response to cadmium ion
dibenzo-p-dioxin metabolic process
drug catabolic process
drug metabolic process
epoxygenase P450 pathway
exogenous drug catabolic process
heterocycle metabolic process
hydrogen peroxide biosynthetic process
lung development
methylation
monocarboxylic acid metabolic process
monoterpenoid metabolic process
omega-hydroxylase P450 pathway
oxidation-reduction process
oxidative deethylation
oxidative demethylation
porphyrin-containing compound metabolic process
post-embryonic development
regulation of gene expression
response to estradiol
response to immobilization stress
response to lipopolysaccharide
small molecule metabolic process
steroid catabolic process
toxin biosynthetic process
xenobiotic metabolic process - Gene Ontology Molecular Function:
- aromatase activity
caffeine oxidase activity
demethylase activity
electron carrier activity
enzyme binding
heme binding
iron ion binding
monooxygenase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen - Gene Ontology Cellular Component:
- endoplasmic reticulum membrane
intracellular membrane-bounded organelle - Keywords:
- 3D-structure
Alternative splicing
Complete proteome
Direct protein sequencing
Endoplasmic reticulum
Glycoprotein
Heme
Iron
Lipid metabolism
Membrane
Metal-binding
Microsome
Monooxygenase
Oxidoreductase
Polymorphism
Reference proteome
Steroid metabolism
Sterol metabolism