Names & Taxonomy
- Uniprot ID:
- O75342
- Entry Name:
- LX12B_HUMAN
- Status:
- reviewed
- Protein Names:
- Arachidonate 12-lipoxygenase, 12R-type (12R-LOX) (12R-lipoxygenase) (EC 1.13.11.-) (Epidermis-type lipoxygenase 12)
- Gene Names:
- ALOX12B
- Gene Names Primary:
- ALOX12B
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 701
- Sequence:
- MATYKVRVATGTDLLSGTRDSISLTIVGTQGESHKQLLNHFGRDFATGAVGQYTVQCPQDLGELIIIRLHKERYAFFPKDPWYCNYVQICAPNGRIYHFPAYQWMDGYETLALREATGKTTADDSLPVLLEHRKEEIRAKQDFYHWRVFLPGLPSYVHIPSYRPPVRRHRNPNRPEWNGYIPGFPILINFKATKFLNLNLRYSFLKTASFFVRLGPMALAFKVRGLLDCKHSWKRLKDIRKIFPGKKSVVSEYVAEHWAEDTFFGYQYLNGVNPGLIRRCTRIPDKFPVTDDMVAPFLGEGTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGKMMPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMCHPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGVQDLPGYYYRDDSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEIFKECLLGRESSGFPRCLRTVPELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMDTLPDVKTTCITLLVLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIRQRNKCLPIPYYYLDPVLIENSISI
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm
Function
- Function:
- Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.
- Pathway:
- Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
- Catalytic Activity:
- Arachidonate + O(2) = (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyicosa-5,8,10,14-tetraenoate.
- Cofactor:
- COFACTOR: Name=Fe cation; Xref=ChEBI:CHEBI:24875;
- Gene Ontology Go:
- cytosol
arachidonate 12-lipoxygenase activity
iron ion binding
linoleate 9S-lipoxygenase activity
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
arachidonic acid metabolic process
ceramide biosynthetic process
establishment of skin barrier
hepoxilin biosynthetic process
linoleic acid metabolic process
lipoxygenase pathway
oxidation-reduction process
positive regulation of gene expression
positive regulation of MAPK cascade
positive regulation of mucus secretion
protein lipidation
small molecule metabolic process
sphingolipid metabolic process - Gene Ontology Biological Process:
- arachidonic acid metabolic process
ceramide biosynthetic process
establishment of skin barrier
hepoxilin biosynthetic process
linoleic acid metabolic process
lipoxygenase pathway
oxidation-reduction process
positive regulation of gene expression
positive regulation of MAPK cascade
positive regulation of mucus secretion
protein lipidation
small molecule metabolic process
sphingolipid metabolic process - Gene Ontology Molecular Function:
- arachidonate 12-lipoxygenase activity
iron ion binding
linoleate 9S-lipoxygenase activity
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen - Gene Ontology Cellular Component:
- cytosol
- Keywords:
- Complete proteome
Cytoplasm
Dioxygenase
Disease mutation
Fatty acid metabolism
Ichthyosis
Iron
Lipid metabolism
Metal-binding
Oxidoreductase
Polymorphism
Reference proteome - Interacts With:
- Q9BYJ1