Names & Taxonomy
- Uniprot ID:
- O60909
- Entry Name:
- B4GT2_HUMAN
- Status:
- reviewed
- Protein Names:
- Beta-1,4-galactosyltransferase 2 (Beta-1,4-GalTase 2) (Beta4Gal-T2) (b4Gal-T2) (EC 2.4.1.-) (UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2) (UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2) [Includes: Lactose synthase A protein (EC 2.4.1.22); N-acetyllactosamine synthase (EC 2.4.1.90) (Nal synthase); Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC 2.4.1.38); Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC 2.4.1.-)]
- Gene Names:
- B4GALT2
- Gene Names Primary:
- B4GALT2
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 372
- Sequence:
- MSRLLGGTLERVCKAVLLLCLLHFLVAVILYFDVYAQHLAFFSRFSARGPAHALHPAASSSSSSSNCSRPNATASSSGLPEVPSALPGPTAPTLPPCPDSPPGLVGRLLIEFTSPMPLERVQRENPGVLMGGRYTPPDCTPAQTVAVIIPFRHREHHLRYWLHYLHPILRRQRLRYGVYVINQHGEDTFNRAKLLNVGFLEALKEDAAYDCFIFSDVDLVPMDDRNLYRCGDQPRHFAIAMDKFGFRLPYAGYFGGVSGLSKAQFLRINGFPNEYWGWGGEDDDIFNRISLTGMKISRPDIRIGRYRMIKHDRDKHNEPNPQRFTKIQNTKLTMKRDGIGSVRYQVLEVSRQPLFTNITVDIGRPPSWPPRG
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Trans cisternae of Golgi stack.
Function
- Function:
- Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose.
- Pathway:
- Protein modification; protein glycosylation.
- Catalytic Activity:
- UDP-alpha-D-galactose + D-glucose = UDP + lactose.; UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.; UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
- Cofactor:
- COFACTOR: Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=71 uM for GlcNAc-B-S-pNP
- Cross Reference Drug Bank:
- DB00141
- Gene Ontology Go:
- Golgi cisterna membrane
Golgi membrane
integral component of membrane
beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity
galactosyltransferase activity
lactose synthase activity
metal ion binding
N-acetyllactosamine synthase activity
carbohydrate metabolic process
cellular protein metabolic process
glycosaminoglycan metabolic process
keratan sulfate biosynthetic process
keratan sulfate metabolic process
post-translational protein modification
protein N-linked glycosylation via asparagine
small molecule metabolic process - Gene Ontology Biological Process:
- carbohydrate metabolic process
cellular protein metabolic process
glycosaminoglycan metabolic process
keratan sulfate biosynthetic process
keratan sulfate metabolic process
post-translational protein modification
protein N-linked glycosylation via asparagine
small molecule metabolic process - Gene Ontology Molecular Function:
- beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity
galactosyltransferase activity
lactose synthase activity
metal ion binding
N-acetyllactosamine synthase activity - Gene Ontology Cellular Component:
- Golgi cisterna membrane
Golgi membrane
integral component of membrane - Keywords:
- Alternative splicing
Complete proteome
Disulfide bond
Glycoprotein
Glycosyltransferase
Golgi apparatus
Manganese
Membrane
Metal-binding
Polymorphism
Reference proteome
Signal-anchor
Transferase
Transmembrane
Transmembrane helix