Names & Taxonomy

Uniprot ID:
O60760
Entry Name:
HPGDS_HUMAN
Status:
reviewed
Protein Names:
Hematopoietic prostaglandin D synthase (H-PGDS) (EC 5.3.99.2) (GST class-sigma) (Glutathione S-transferase) (EC 2.5.1.18) (Glutathione-dependent PGD synthase) (Glutathione-requiring prostaglandin D synthase) (Prostaglandin-H2 D-isomerase)
Gene Names:
HPGDS GSTS PGDS PTGDS2
Gene Names Primary:
HPGDS
Organism:
Homo sapiens (Human)

Structure

Length:
199
Sequence:
MPNYKLTYFNMRGRAEIIRYIFAYLDIQYEDHRIEQADWPEIKSTLPFGKIPILEVDGLTLHQSLAIARYLTKNTDLAGNTEMEQCHVDAIVDTLDDFMSCFPWAEKKQDVKEQMFNELLTYNAPHLMQDLDTYLGGREWLIGNSVTWADFYWEICSTTLLVFKPDLLDNHPRLVTLRKKVQAIPAVANWIKRRPQTKL
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm

Function

Function:
Bifunctional enzyme which catalyzes both the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation, and the conjugation of glutathione with a wide range of aryl halides and organic isothiocyanates. Also exhibits low glutathione-peroxidase activity towards cumene hydroperoxide.
Catalytic Activity:
(5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate.
Cofactor:
COFACTOR: Name=glutathione; Xref=ChEBI:CHEBI:57925;
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=8 mM for glutathione for the glutathione-conjugating activity
Enzyme Regulation:
ENZYME REGULATION: Prostaglandin PGD2 synthesis is stimulated by calcium and magnesium ions. One calcium or magnesium ion is bound between the subunits of the homodimer. The interactions with the protein are for the most part mediated via water molecules. Magnesium increases the affinity for glutathione, while calcium has no effect on the affinity for glutathione.
Cross Reference Drug Bank:
DB00143 DB07615
Gene Ontology Go:
cytoplasm
cytosol
calcium ion binding
glutathione transferase activity
magnesium ion binding
prostaglandin-D synthase activity
protein homodimerization activity
arachidonic acid metabolic process
cyclooxygenase pathway
glutathione derivative biosynthetic process
locomotory behavior
negative regulation of male germ cell proliferation
prostaglandin metabolic process
signal transduction
small molecule metabolic process
xenobiotic metabolic process
Gene Ontology Biological Process:
arachidonic acid metabolic process
cyclooxygenase pathway
glutathione derivative biosynthetic process
locomotory behavior
negative regulation of male germ cell proliferation
prostaglandin metabolic process
signal transduction
small molecule metabolic process
xenobiotic metabolic process
Gene Ontology Molecular Function:
calcium ion binding
glutathione transferase activity
magnesium ion binding
prostaglandin-D synthase activity
protein homodimerization activity
Gene Ontology Cellular Component:
cytoplasm
cytosol
Keywords:
3D-structure
Calcium
Complete proteome
Cytoplasm
Fatty acid biosynthesis
Fatty acid metabolism
Isomerase
Lipid biosynthesis
Lipid metabolism
Magnesium
Metal-binding
Prostaglandin biosynthesis
Prostaglandin metabolism
Reference proteome
Transferase
Interacts With:
Q96GS6; Q96B67; P08582-2

Publication

PubMed ID:
10824118 11672424 14702039 15489334 9425264 9353279 12627223 15113825 16547010 18341273 19939518