Names & Taxonomy

Uniprot ID:
O60568
Entry Name:
PLOD3_HUMAN
Status:
reviewed
Protein Names:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 (EC 1.14.11.4) (Lysyl hydroxylase 3) (LH3)
Gene Names:
PLOD3
Gene Names Primary:
PLOD3
Organism:
Homo sapiens (Human)

Structure

Length:
738
Sequence:
MTSSGPGPRFLLLLPLLLPPAASASDRPRGRDPVNPEKLLVITVATAETEGYLRFLRSAEFFNYTVRTLGLGEEWRGGDVARTVGGGQKVRWLKKEMEKYADREDMIIMFVDSYDVILAGSPTELLKKFVQSGSRLLFSAESFCWPEWGLAEQYPEVGTGKRFLNSGGFIGFATTIHQIVRQWKYKDDDDDQLFYTRLYLDPGLREKLSLNLDHKSRIFQNLNGALDEVVLKFDRNRVRIRNVAYDTLPIVVHGNGPTKLQLNYLGNYVPNGWTPEGGCGFCNQDRRTLPGGQPPPRVFLAVFVEQPTPFLPRFLQRLLLLDYPPDRVTLFLHNNEVFHEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKLWSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Rough endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side.

Function

Function:
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
Catalytic Activity:
L-lysine- + 2-oxoglutarate + O(2) = (2S,5R)-5-hydroxy-L-lysine- + succinate + CO(2).
Cofactor:
COFACTOR: Name=Fe cation; Xref=ChEBI:CHEBI:24875; ; COFACTOR: Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
Active Site:
ACT_SITE 729 729
Cross Reference Drug Bank:
DB00139 DB00126
Gene Ontology Go:
endoplasmic reticulum
endoplasmic reticulum membrane
extracellular exosome
rough endoplasmic reticulum membrane
iron ion binding
L-ascorbic acid binding
procollagen galactosyltransferase activity
procollagen glucosyltransferase activity
procollagen-lysine 5-dioxygenase activity
basement membrane assembly
cellular protein modification process
cellular response to hormone stimulus
collagen fibril organization
endothelial cell morphogenesis
epidermis morphogenesis
extracellular matrix organization
in utero embryonic development
lung morphogenesis
neural tube development
protein localization
vasodilation
Gene Ontology Biological Process:
basement membrane assembly
cellular protein modification process
cellular response to hormone stimulus
collagen fibril organization
endothelial cell morphogenesis
epidermis morphogenesis
extracellular matrix organization
in utero embryonic development
lung morphogenesis
neural tube development
protein localization
vasodilation
Gene Ontology Molecular Function:
iron ion binding
L-ascorbic acid binding
procollagen galactosyltransferase activity
procollagen glucosyltransferase activity
procollagen-lysine 5-dioxygenase activity
Gene Ontology Cellular Component:
endoplasmic reticulum
endoplasmic reticulum membrane
extracellular exosome
rough endoplasmic reticulum membrane
Keywords:
Complete proteome
Dioxygenase
Disease mutation
Endoplasmic reticulum
Glycoprotein
Iron
Membrane
Metal-binding
Oxidoreductase
Polymorphism
Reference proteome
Signal
Vitamin C
Interacts With:
Q5JST6; A2ABF9; Q96QF0; Q9BQY4

Publication

PubMed ID:
9582318 9724729 10686427 14702039 12853948 15489334 21269460 25944712 18834968