Names & Taxonomy

Uniprot ID:
O43681
Entry Name:
ASNA_HUMAN
Status:
reviewed
Protein Names:
ATPase ASNA1 (EC 3.6.-.-) (Arsenical pump-driving ATPase) (Arsenite-stimulated ATPase) (Transmembrane domain recognition complex 40 kDa ATPase subunit) (hARSA-I) (hASNA-I)
Gene Names:
ASNA1 ARSA TRC40
Gene Names Primary:
ASNA1
Organism:
Homo sapiens (Human)

Structure

Length:
348
Sequence:
MAAGVAGWGVEAEEFEDAPDVEPLEPTLSNIIEQRSLKWIFVGGKGGVGKTTCSCSLAVQLSKGRESVLIISTDPAHNISDAFDQKFSKVPTKVKGYDNLFAMEIDPSLGVAELPDEFFEEDNMLSMGKKMMQEAMSAFPGIDEAMSYAEVMRLVKGMNFSVVVFDTAPTGHTLRLLNFPTIVERGLGRLMQIKNQISPFISQMCNMLGLGDMNADQLASKLEETLPVIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSAQ
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm. Endoplasmic reticulum. Nucleus, nucleolus.

Function

Function:
ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (By similarity). May be involved in insulin signaling.
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.22 mM for ATP
Active Site:
ACT_SITE 74 74
Cross Reference Drug Bank:
DB00171
Gene Ontology Go:
cytoplasm
endoplasmic reticulum membrane
extracellular exosome
nucleolus
nucleus
arsenite transmembrane transporter activity
ATP binding
ATPase activity
metal ion binding
transporter activity
cellular protein metabolic process
endoplasmic reticulum unfolded protein response
inorganic anion transport
IRE1-mediated unfolded protein response
protein insertion into ER membrane
transport
Gene Ontology Biological Process:
cellular protein metabolic process
endoplasmic reticulum unfolded protein response
inorganic anion transport
IRE1-mediated unfolded protein response
protein insertion into ER membrane
transport
Gene Ontology Molecular Function:
arsenite transmembrane transporter activity
ATPase activity
ATP binding
metal ion binding
transporter activity
Gene Ontology Cellular Component:
cytoplasm
endoplasmic reticulum membrane
extracellular exosome
nucleolus
nucleus
Keywords:
ATP-binding
Acetylation
Complete proteome
Cytoplasm
Endoplasmic reticulum
Hydrolase
Metal-binding
Nucleotide-binding
Nucleus
Polymorphism
Reference proteome
Transport
Zinc
Interacts With:
Q12797; Q96SL4; Q9Y5L2

Publication

PubMed ID:
14702039 15057824 15489334 8884272 9712828 9736449 9774623 17382883 18477612 19413330 21269460 21444755 24275569