Names & Taxonomy
- Uniprot ID:
- Q9Y697
- Entry Name:
- NFS1_HUMAN
- Status:
- reviewed
- Protein Names:
- Cysteine desulfurase, mitochondrial (EC 2.8.1.7)
- Gene Names:
- NFS1 NIFS HUSSY-08
- Gene Names Orf:
- HUSSY-08
- Gene Names Primary:
- NFS1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 457
- Sequence:
- MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Isoform Mitochondrial: Mitochondrion.; Isoform Cytoplasmic: Cytoplasm. Nucleus.
Function
- Function:
- Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor.
- Catalytic Activity:
- L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.
- Cofactor:
- COFACTOR: Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: ; Kinetic parameters: KM=434.75 uM for L-cysteine
- Active Site:
- ACT_SITE 381 381 Cysteine persulfide intermediate.
- Cross Reference Drug Bank:
- DB00160 DB00151
- Gene Ontology Go:
- cytoplasm
cytosol
intracellular membrane-bounded organelle
mitochondrial matrix
mitochondrion
nucleolus
nucleoplasm
nucleus
cysteine desulfurase activity
iron-sulfur cluster binding
metal ion binding
protein homodimerization activity
pyridoxal phosphate binding
cluster assembly
iron incorporation into metallo-sulfur cluster
Mo-molybdopterin cofactor biosynthetic process
molybdopterin cofactor biosynthetic process
protein complex assembly
small molecule metabolic process
sulfur amino acid metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process - Gene Ontology Biological Process:
- cluster assembly
iron incorporation into metallo-sulfur cluster
molybdopterin cofactor biosynthetic process
Mo-molybdopterin cofactor biosynthetic process
protein complex assembly
small molecule metabolic process
sulfur amino acid metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process - Gene Ontology Molecular Function:
- cysteine desulfurase activity
iron-sulfur cluster binding
metal ion binding
protein homodimerization activity
pyridoxal phosphate binding - Gene Ontology Cellular Component:
- cytoplasm
cytosol
intracellular membrane-bounded organelle
mitochondrial matrix
mitochondrion
nucleolus
nucleoplasm
nucleus - Keywords:
- Alternative initiation
Alternative splicing
Complete proteome
Cytoplasm
Iron
Iron-sulfur
Metal-binding
Mitochondrion
Molybdenum cofactor biosynthesis
Nucleus
Pyridoxal phosphate
Reference proteome
Transferase
Transit peptide